Abstract
Nuclear factor kappa B (NFκB) is an evolutionary conserved transcription factor, which coordinates various metabolic processes triggered by innate and adaptive immune responses. Supakar et al. (J. Biol. Chem. 270: 837–842, 1995) had reported a 10-fold increase in DNA binding activity of NFκB in liver of old rats. In this study, we have analyzed the changes in the level of NFκB, inhibitor of NFκB (IκBα), phosphorylated-IκBα (p-IκBα) and IκB kinase (IKK) in rat liver during aging by reverse transcription polymerase chain reaction and/or western blotting. Here we demonstrate that there is an age-dependent increase in the level of p-IκBα with concomitant decrease in the level of IκBα, which may be correlated with increased inflammation, oxidative stress and higher level of activated NFκB in rat liver in old age.
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Abbreviations
- AR:
-
androgen receptor
- NFκB:
-
nuclear factor kappa B
- IκBα:
-
inhibitor of NFκB
- p-IκBα:
-
phosphorylated inhibitor of NFκB
- IKK:
-
IκB kinase
- EMSA:
-
electrophoretic mobility shift assay
- RT-PCR:
-
reverse transcription polymerase chain reaction
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Zaidi, G., Panda, H. & Supakar, P.C. Increased phosphorylation and decreased level of IκBα during aging in rat liver. Biogerontology 6, 141–145 (2005). https://doi.org/10.1007/s10522-005-3459-5
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DOI: https://doi.org/10.1007/s10522-005-3459-5