We studied the effect of laser radiation of moderate intensity with a wavelength of 970 nm on the efficiency of colony formation of rat bone marrow mesenchymal stem cells (MSC) in vitro. In this case, photobimodulation and thermal heating of MSC occur simultaneously. This combined laser treatment allows increasing the number of colonies by 6 times in comparison with the control and by more than 3 times in comparison with thermal heating alone. The mechanism of such an increase is associated with combined thermal and light effects of laser radiation of moderate intensity, which stimulates cell proliferation. This phenomenon can be used as the basis for solving the most important task of cell transplantation, associated with the expansion of autologous stem cells and activation of their proliferative potential.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Rodríguez-Fuentes DE, Fernández-Garza LE, Samia-Meza JA, Barrera-Barrera SA, Caplan AI, Barrera-Saldaña HA. Mesenchymal stem cells current clinical applications: a systematic review. Arch. Med. Res. 2021;52(1):93-101. https://doi.org/10.1016/j.arcmed.2020.08.006
Bochkov NP, Nikitina VA. Cytogenetics of human stem cells. Mol. Med. 2008;(3):40-47. Russian.
Wang Y, Zhang Z, Chi Y, Zhang Q, Xu F, Yang Z, Meng L, Yang S, Yan S, Mao A, Zhang J, Yang Y, Wang S, Cui J, Liang L, Ji Y, Han ZB, Fang X, Han ZC. Long-term cultured mesenchymal stem cells frequently develop genomic mutations but do not undergo malignant transformation. Cell Death Dis. 2013;4(12):e950. https://doi.org/10.1038/cddis.2013.480
Bikmulina PY, Kosheleva NV, Shpichka AI, Efremov YM, Yusupov VI, Timashev PS, Rochev YA. Beyond 2D: effects of photobiomodulation in 3D tissue-like systems. J. Biomed. Opt. 2020;25(4):1-16. https://doi.org/10.1117/1.JBO.25.4.048001
Pasternak-Mnich K, Ziemba B, Szwed A, Kopacz K, Synder M, Bryszewska M, Kujawa J. Effect of photobiomodulation therapy on the increase of viability and proliferation of human mesenchymal stem cells. Lasers Surg. Med. 2019;51(9):824-833. https://doi.org/10.1002/lsm.23107
Pinto H, Goñi Oliver P, Sánchez-Vizcaíno Mengual E. The Effect of photobiomodulation on human mesenchymal cells: a literature review. Aesthetic Plast. Surg. 2021;45(4):1826-1842. https://doi.org/10.1007/s00266-021-02173-y
Bayat M, Virdi A, Rezaei F, Chien S. Comparison of the in vitro effects of low-level laser therapy and low-intensity pulsed ultrasound therapy on bony cells and stem cells. Prog. Biophys. Mol. Biol. 2018;133:36-48. https://doi.org/10.1016/j.pbiomolbio.2017.11.001
Reissis Y, García-Gareta E, Korda M, Blunn GW, Hua J. The effect of temperature on the viability of human mesenchymal stem cells. Stem Cell Res. Ther. 2013;4(6):139. https://doi.org/10.1186/scrt350
Chaikhalyan RK, Yusupov VI, Gorskaya YF, Kuralesova AI, Gerasimov YV, Sviridov AP, Tambiev AKh, Vorob’eva NN, Shishkova AG, Moskvina IL, Bagratashvili VN. Effects of acoustic and EHF impulses on multipotent stromal cells during formation of bone marrow containing heterotopic organs in tissue engineered constructions. Bull. Exp. Biol. Med. 2015;158(5):688-691. https://doi.org/10.1007/s10517-015-2836-6
Castilla-Casadiego DA, Reyes-Ramos AM, Domenech M, Almodovar J. Effects of physical, chemical, and biological stimulus on h-MSC expansion and their functional characteristics. Ann. Biomed. Eng. 2020;48(2):519-535. https://doi.org/10.1007/s10439-019-02400-3
Chailakhyan RK, Grosheva AG, Vorobyeva NN, Yusupov VI, Genkina EN, Sviridov AP. Changes in the number of mesenchymal stem cells in bone marrow at different periods after in vivo exposure of the bone marrow to local infrared laser radiation. Bull. Exp. Biol. Med. 2022;173(1):119-122. https://doi.org/10.1007/s10517-022-05505-3
Tuby H, Maltz L, Oron U. Low-level laser irradiation (LLLI) promotes proliferation of mesenchymal and cardiac stem cells in culture. Lasers Surg. Med. 2007;39(4):373-378. https://doi.org/10.1002/lsm.20492
Chailahyan RK, Gerasimov JuV, Sviridov AP, Kondjurin AV, Tambiev AH, Bagratishvili VN. Effect of IR laser radiation on the multipotent mesenchymal stromal stem cells of rat marrow in vivo. Ross. Immunol. Zh. 2009;3(3-4):333-337. Russian.
Zamani ARN, Saberianpour S, Geranmayeh MH, Bani F, Haghighi L, Rahbarghazi R. Modulatory effect of photobiomodulation on stem cell epigenetic memory: a highlight on differentiation capacity. Lasers Med. Sci. 2020;35(2):299-306. https://doi.org/10.1007/s10103-019-02873-7
Wang Y, Huang YY, Wang Y, Lyu P, Hamblin MR. Photobiomodulation of human adipose-derived stem cells using 810 nm and 980 nm lasers operates via different mechanisms of action. Biochim. Biophys. Acta Gen. Subj. 2017;1861(2):441-449. https://doi.org/10.1016/j.bbagen.2016.10.008
Andreeva NV, Belyavsky AV, Yusupov VI, Timashev PS, Zotov KV. Effects of infrared laser irradiation on aging of mesenchymal stem cells in culture. Optical Engineering. 2019;59(6):061607. https://doi.org/10.1117/1.OE.59.6.061607
Author information
Authors and Affiliations
Corresponding author
Additional information
Translated from Kletochnye Tekhnologii v Biologii i Meditsine, No. 4, pp. 212-215, December, 2022
Rights and permissions
Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.
About this article
Cite this article
Chailakhyan, R.K., Grosheva, A.G., Vorob’eva, N.N. et al. Laser Thermo-Photobiomodulation of Bone Marrow Mesenchymal Stem Cells. Bull Exp Biol Med 174, 523–526 (2023). https://doi.org/10.1007/s10517-023-05741-1
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10517-023-05741-1