We studied the effects of pregnancy-specific β1-glycoprotein (PSG) on the replicative potential of naïve T cells (CD45RA+) and immune memory T cells (CD45R0+) in vitro by evaluating the expression of the hTERT gene in combination with the proliferative activity of cells. Human PSG was obtained by the author’s patented method of immunopurification using a biospecific sorbent with subsequent removal of immunoglobulin contamination on a HiTrap Protein G HP column. We used monocultures of CD45RA+ and CD45R0+ lymphocytes isolated from peripheral blood mononuclear cells of reproductive-age women. It was found that PSG in physiological concentrations inhibited the expression of the hTERT gene mRNA in naïve T cells and immune memory T cells and simultaneously reduced the number of proliferating T cells estimated by the differential gating method. At the same time, PSG reduced CD71 expression only on naïve T cells without affecting this molecule on immune memory T cells. Thus, PSG decreased the replication potential and suppressed the proliferation of T cells and immune memory T cells, which in the context of pregnancy can contribute to the formation of immune tolerance to the semi-allogeneic embryo.
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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 172, No. 8, pp. 198-204, August, 2021
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Timganova, V.P., Litvinova, L.S., Yurova, K.A. et al. Effect of Pregnancy Specific β1-Glycoprotein on the Replicative Potential of Naïve T Cells and Immune Memory T Cells. Bull Exp Biol Med 172, 169–174 (2021). https://doi.org/10.1007/s10517-021-05357-3
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DOI: https://doi.org/10.1007/s10517-021-05357-3