We propose an optimized single-step assay of total nitrite and nitrate concentrations based on serum deproteinization by ultrafiltration. After deproteinization, the residual protein concentration was 0.0800±0.0007 mg/ml, which secured necessary transparency of the specimens in contrast to methanol deproteinization. The optimized assay for serum nitrite and nitrate ions is characterized by high reproducibility and accuracy (variation coefficient <7%) and can be recommended for routine use in clinical practice.
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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 165, No. 2, pp. 252-256, February, 2018
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Gumanova, N.G., Klimushina, M.V. & Metel’skaya, V.A. Optimization of Single-Step Assay for Circulating Nitrite and Nitrate Ions (NOx) as Risk Factors of Cardiovascular Mortality. Bull Exp Biol Med 165, 284–287 (2018). https://doi.org/10.1007/s10517-018-4149-z
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DOI: https://doi.org/10.1007/s10517-018-4149-z