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Cytokine Production in Mixed Cultures of Mesenchymal Stromal Cells from Wharton’s Jelly and Peripheral Blood Lymphocytes

  • Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine)
  • Published:
Bulletin of Experimental Biology and Medicine Aims and scope

We compared the production of 19 humoral factors in mixed cultures of mesenchymal stromal cells from Wharton’s jelly and allogenic peripheral blood lymphocytes. For evaluation of the specificity of immunosuppressive activity of mesenchymal stromal cells, comparative analysis of the production of these humoral factors in mixed cultures of lymphocytes and epithelial BxPC-3 cells was conducted. The production of soluble factors in both mono- and mixed cultures significantly correlated (p<0.05). The maximum production was found for proinflammatory chemokine IP-10 and IFN-γ and anti-inflammatory cytokine IL-10. The major difference of mesenchymal stromal cells from epithelial BxPC-3 cells was 7-fold higher production of IL-10, which can explain the immunosuppressive effect of mesenchymal stromal cells.

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Authors and Affiliations

  1. Institute of Applied Mechanics, Russian Academy of Sciences, Moscow, Russia

    A. M. Poltavtsev & N. E. Volgina

  2. V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation, Moscow, Russia

    R. A. Poltavtseva & M. N. Yushina

  3. M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia

    E. V. Svirshchevskaya

Authors
  1. A. M. Poltavtsev
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  2. R. A. Poltavtseva
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  3. M. N. Yushina
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  4. N. E. Volgina
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  5. E. V. Svirshchevskaya
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Correspondence to E. V. Svirshchevskaya.

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Translated from Kletochnye Tekhnologii v Biologii i Meditsine, No. 1, pp. 61-68, January, 2017

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Poltavtsev, A.M., Poltavtseva, R.A., Yushina, M.N. et al. Cytokine Production in Mixed Cultures of Mesenchymal Stromal Cells from Wharton’s Jelly and Peripheral Blood Lymphocytes. Bull Exp Biol Med 163, 169–175 (2017). https://doi.org/10.1007/s10517-017-3759-1

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  • DOI: https://doi.org/10.1007/s10517-017-3759-1

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