Antioxidant properties of recombinant peroxiredoxin-6 and chimeric protein PSH combining peroxidase and superoxide dismutase activities were studied on the model of retrograde perfusion of isolated rat heart under conditions of H2O2-induced oxidative stress. The exogenous antioxidant proteins exhibited cardioprotective properties manifested in heart rate normalization, maintenance of contractile activity of the myocardium, and prevention of H2O2-induced LPO in oxidative stress. Localization of peroxiredoxin-6 and PSH in the cardiac tissue was determined and myocardial structures most effectively protected by the antioxidant enzymes from ischemia/reperfusion-induced damages were identified. The results suggest that modified peroxiredoxins are promising components of perfusion media for preservation of isolated organs.
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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 160, No. 11, pp. 584-588, November, 2015
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Karaduleva, E.V., Mubarakshina, E.K., Sharapov, M.G. et al. Cardioprotective Effect of Modified Peroxiredoxins in Retrograde Perfusion of Isolated Rat Heart under Conditions of Oxidative Stress. Bull Exp Biol Med 160, 639–642 (2016). https://doi.org/10.1007/s10517-016-3237-1
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DOI: https://doi.org/10.1007/s10517-016-3237-1