Aqueous suspension of magnetite nanoparticles with primary diameter of 10 nm were intratracheally administered into rat lungs. In 24 h, cells were isolated from bronchoalveolar lavage and examined under a transmission electron microscope. Alveolar macrophages demonstrated ability to actively uptake single nanoparticles and small aggregates composed of such particles, which then formed larger conglomerates inside fused phagosomes. Some of these mature phagosomes shed the membrane and free nanoparticles closely interacted with nuclear membrane and with cristae and mitochondrial membranes thereby inflicting pronounced damage to these intracellular structures. The loss of primary lysosomes can be viewed as indirect evidence attesting to the role played by diffusion of lysosomal hydrolytic enzymes in the final destruction of the alveolar macrophages provoked by nanoparticles.
Similar content being viewed by others
References
B. A. Katsnelson, O. G. Alekseeva, L. I. Privalova, and E. V. Polzik, Pneumoconioses: Pathogenesis and Biological Prevention [in Russian], Ekaterinburg (1995).
B. A. Katsnelson, L. I. Privalova, S. V. Kuzmin, et al., Toksikol. Vestn., No. 2, 17–24 (2010).
K. Donaldson, V. Stone, C. L. Tran, et al., Occup. Environ. Med., 61, No. 9, 727–728 (2004).
B. A. Katsnelson, L. I. Privalova, S. V. Kuzmin, et al., Int. J. Occup. Environ. Health., 16, No. 4, 508–524 (2010).
R. Kjeken, M. Egeberg, A. Habermann, et al., Mol. Biol. Cell, 15, No. 1, 345–348 (2004).
N. Li, C. Sioutas, A. Cho, et al., Environ. Health Perspect., 111, No. 4, 455–460 (2003).
N. Li, T. Xia, and A. E. Nel, Free Radic. Biol. Med., 44, No. 9, 1689–1699 (2008).
G. Oberdörster, E. Oberdörster, and J. Oberdörster, Environ. Health Perspect., 113, No. 7, 823–839 (2005).
Author information
Authors and Affiliations
Corresponding author
Additional information
Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 152, No. 11, pp. 560-563, November, 2011
Rights and permissions
About this article
Cite this article
Katsnelson, B.A., Privalova, L.I., Sutunkova, M.P. et al. Interaction of Iron Oxide Fe3O4 Nanoparticles and Alveolar Macrophages in Vivo . Bull Exp Biol Med 152, 627–629 (2012). https://doi.org/10.1007/s10517-012-1593-z
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10517-012-1593-z