Skip to main content
Log in

Isolation of Insulin-Producing Cells from Different Populations of Multipotent Stromal Cells of the Umbilical Cord and Human Adipose Tissue

  • Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine)
  • Published:
Bulletin of Experimental Biology and Medicine Aims and scope

Stromal cells of adipose tissue and human umbilical cord were isolated by the original method from general populations of multipotent subpopulation of multipotent stromal cells exhibiting perivascular phenotype (CD146+, CD31). Effective directed differentiation of these cells into insulin-producing cells by transient transfection of the gene Pdx1 was demonstrated. Transfection multipotent stromal cells CD146, CD31 derived from adipose tissue and umbilical cord and isolated by the standard method, did not result in activation of insulin gene transcription. It was shown that the expression of nestin was not necessary for effective pancreatic cell differentiation.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. A. A. Rzhaninova, A. V. Kulikov, I. A. Spirova, et al., Klet. Tekhn. Biol. Med., No. 1, 3–9 (2010).

  2. P. H. Ashjian, A. S. Elbarbary, B. Edmonds, et al., Plast. Reconstr. Surg., 111, No. 6, 1922–1931 (2003).

    Article  PubMed  Google Scholar 

  3. S. Assady, G. Maor, M. Amit, et al., Diabetes, 50, No. 8, 1691–1697 (2001).

    Article  PubMed  CAS  Google Scholar 

  4. A. Caplan, J. Pathol., 217, No. 2, 318–324 (2009).

    Article  PubMed  CAS  Google Scholar 

  5. J. Czyz, C. Wiese, A. Rolletscher, et al., Biol. Chem., 384, Nos. 10–11, 1391–1409 (2003).

    Article  PubMed  CAS  Google Scholar 

  6. T. Dvash and N. Benvenisty, Best Pract. Res. Clin. Obstet. Gynaecol., 18, No. 6, 929–940 (2004).

    Article  PubMed  Google Scholar 

  7. S. Miyazaki, E.Yamato, and J. Miyazaki, Diabetes, 53, No. 4, 1030–1037 (2004).

    Article  PubMed  CAS  Google Scholar 

  8. H. Ning, G. Lin, T. F. Lue, and C. S. Lin, Differentiation, 74, Nos. 9–10, 510–518 (2006).

    Article  PubMed  CAS  Google Scholar 

  9. J. Rajagopal, W. J. Anderson, S. Kume, et al., Science, 299, No. 5605, 363 (2003).

    PubMed  Google Scholar 

  10. A. Sharma, D. H. Zangen, P. Reitz, et al., Diabetes, 48, No. 3, 507–513 (1999).

    Article  PubMed  CAS  Google Scholar 

  11. B. Soria, E. Roche, G. Berna, et al., Diabetes, 49, No. 2, 157–162 (2000).

    Article  PubMed  CAS  Google Scholar 

  12. D. A. Stoffers, R. S. Heller, C. P. Miller, and J. F. Habener, Endocrinology, 140, No. 11, 5374–5381 (1999).

    Article  PubMed  CAS  Google Scholar 

  13. D. L. Troyer and M. L. Weiss, Stem Cells, 26, No. 3, 591–599 (2008).

    Article  PubMed  Google Scholar 

  14. P. A .Zuk, M. Zhu, P. Ashjian, et al., Mol. Biol. Cell., 13, No. 12, 4279–4295 (2002).

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to I. A. Fedyunina.

Additional information

Translated from Kletochnye Tekhnologii v Biologii i Meditsine, No. 1, pp. 10–16, January, 2011

Rights and permissions

Reprints and permissions

About this article

Cite this article

Fedyunina, I.A., Rzhaninova, A.A., Kirienko, E.E. et al. Isolation of Insulin-Producing Cells from Different Populations of Multipotent Stromal Cells of the Umbilical Cord and Human Adipose Tissue. Bull Exp Biol Med 151, 114–120 (2011). https://doi.org/10.1007/s10517-011-1279-y

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10517-011-1279-y

Key Words

Navigation