The aim of this research is experimental investigation of the topography and evaluation of some parameters of artificial microterritories promoting osteogenic differentiation of stromal stem cells. A technique of short-term culturing of prenatal human lung stromal cells with fibroblastoid morphology on calcium phosphate substrates with known topography was used. Judging from secretory activity of the cell culture (osteocalcin, alkaline phosphatase), stromal stem cells directly interacting with calcium phosphate discs have advantage in manifestation of osteoblast-like functional activity in comparison with cells cultured on plastic. Rough surfaces of calcium phosphate discs stimulate the formation of spatial human fibroblastoid cell culture. The cells with positive reaction to acid phosphatase are located on spheroliths forming the relief of calcium phosphate coatings. The cells with positive reaction to alkaline phosphatase (marker of osteoblasts) populate hollows (niches) of the artifi cial surface. The niche for induction of osteogenic differentiation of human multipotent mesenchymal stem cells is apparently a structural and functional formation. It can be characterized by an index calculated as the ratio of the total area occupied by alkaline phosphatase-positive cells to the area of artifi cial surface occupied by one stained cell.
Similar content being viewed by others
References
Introduction into Methods of Cell Culture and Bioengineering of Organs and Tissues, Ed. V. V. Novitskii, et al. [in Russian], Tomsk (2004).
O. I. Krivosheina, I. V. Zapuskalov, and I. A. Khlusov, Byull. Eksp. Biol. Med., 139, No. 3, 357–360 (2005).
V. M. Lifshits and V. I. Sidel’nikova, Medical Laboratory Tests: Reference Book [in Russian], Second edition, Moscow (2003).
B. L. Riggs and L. J. Melton 3rd, Osteoporosis [Russian translation], St. Petersburg (2000).
V. I. Rugal’, T. S. Blinova, V. M. Ponomarenko, and K. M. Abdulkadyrov, Gematol. Transfusiol., No. 3, 11–15 (1991).
V. V. Serov and A. B. Shekhter, Connective Tissue (Functional Morphology and General Pathology) [in Russian], Moscow (1981).
N. Tits, Clinical Guide for Laboratory Tests, Ed. V. V. Men’- shikov [in Russian], Moscow (2003).
R. Freshny, Animal Cell Culture. A Practical Approach [Russian translation], Moscow (1989).
F. G. J. Hayhoe and D. Quaglino, Hematological Cytochemistry [Russian translation], Moscow (1983).
I. A. Khlusov, A.V. Karlov, Yu. P. Sharkeev, et al., Klet. Tekhnol. Biol. Med., No. 3, 164–173 (2005).
M. V. Chaikina, I. A. Khlusov, and A. V. Karlov, Khim. v Inter. Ustoich, Razvit., 12, 389–399 (2004).
I. L. Chertkov, N. I. Drize, and A. I. Vorob’ev, Ter. Arkhiv, No. 7, 5–12 (2006).
I. L. Chertkov and A. Ya. Fridenshtein, Cellular Bases of Hemopoiesis, Moscow (1977).
F. Aerts and G. Wagemaker, Genetic Engineering of Mesenchymal Stem Cells. Ed. J.A.Nolta. Springer (2006), pp. 1–44.
Biomaterials Science: an Introduction to Materials in Medicine, Eds. B. D. Ratner, et al., Elsevier Inc. (2004).
B. D. Boyan, S. Lossdorfer, L. Wang, et al., Eur. Cells Mater. 6, 22–27 (2003).
P. R. Crocker and S. Gordon, J. Exp. Med., 162, No. 3, 993–1014 (1985).
L. da Silva Meirelles, P. C. Chagastelles, and N. B. Nardi, J. Cell Sci., 119, 2204–2213 (2006).
L. Di-Silvio and N. Gurav, Human Cell Culture., Eds. M. R. Koller, et al., Kluwer Academic Publishers (2002), Vol. 5, pp. 221–241.
L. M. Eisenberg and C. A. Eisenberg, Birth Defects Res. C. Embryo Today., 69, No. 3, 209–218 (2003).
Q. He, C. Wan, and G. Li, Stem Cells, 25, No. 1, 69–77 (2007).
I. A. Khlusov, L. V. Zagrebin, S. S. Shestov, S. A. Naumov, Stem Cell Applications in Disease and Health, Eds. W. B. Burnsides, et al., New York (2008), pp. 37–80.
V. N. Lama, L. Smith, L. Badri, et al., J. Clin. Invest., 117, No. 4, 989–996 (2007).
U. Meyer, A. Buchter, H. P. Wiesmann, et al., Eur. Cell Mater., 9, 39–49 (2005).
A. Muraglia, R. Cancedda, and R. Quarto, J. Cell Sci., 113, Pt. 7, 1161–1166 (2000).
N. Beyer Nardi and L. da Silva Meirelles and, HEP, 174, 249–282 (2006).
R. Schofi eld, Blood Cells, 4, Nos. 1–2, 7–25 (1978).
R. S. Taichman, Blood, 105, No. 7, 2631–2639 (2005).
C. M. Verfaillie, Trends Cell Biol., 12, 502–508 (2002).
B. M. Wiemann, D. Bingmann, and S. Franzka, Advanced Engineering Materials, 9, No. 12, 1077–1081 (2007).
T. Yin and L. Li, J. Clin. Invest., 116, No. 5, 1195–1201 (2006).
Author information
Authors and Affiliations
Corresponding author
Additional information
Translated from Kletochnye Tekhnologii v Biologii i Meditsine, No. 4, pp. 216–224, October, 2010
Rights and permissions
About this article
Cite this article
Khlusov, I.A., Khlusova, M.Y., Zaitsev, K.V. et al. Pilot in vitro study of the parameters of artificial niche for osteogenic differentiation of human stromal stem cell pool. Bull Exp Biol Med 150, 535–542 (2011). https://doi.org/10.1007/s10517-011-1184-4
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10517-011-1184-4