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Expression of reproduction and antioxidant-related genes in crayfish Cherax quadricarinatus fed with dietary feed additives

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Abstract

We evaluated the impact of a combined vitamin E (vit E), a mixture of nucleotide (mixed-NT), Haematococcus pluvialis (H. pluvialis), arachidonic acid (AA), and yeast extract powder (YEP) supplementations on the relative mRNA expression of reproduction and antioxidant-related genes in red claw crayfish Cherax quadricarinatus using an orthogonal design (L16 45) plus control diet for 70 days feeding trial. Five factors with four levels: A, vit E 40, 160, 240, and 320 mg/kg diet; B, mixed-NT 2, 4, 6, and 8 g/kg diet; C, H. pluvialis 2, 4, 6, and 8 g/kg diet; D, AA 5, 10, 15, and 20 g/kg diet; E, YEP 2.5, 5, 10, and 15 g/kg diet were studied. The relative mRNA expression of reproduction-related genes (vitellogenin (vtg) and fatty acid-binding protein (fabp)), and antioxidant-related genes (heat shock protein 70 (hsp70), glutathione peroxidase (gpx), glutathione S-transferase (gst), mitochondrial manganese superoxide dismutase (mmnsod), and ferritin) in hemocyte, hepatopancreas, and ovary tissues were measured. There was a major depression in gene expression levels throughout the experimental diet at high vit E 320 mg/kg level with other factors. Expression of antioxidant enzyme genes significantly increased in treatments of T9, T10, T11, and T12, while significantly decreasing in treatment of T13 and other treatment groups with high vit E 320 mg/kg levels with the other factors. Gene expression of vtg and fabp demonstrated similar patterns to those of antioxidant genes. These results provide evidence that combined supplementations of vit E, mixed-NT, H. pluvialis, AA, and YEP with vit E 240 mg/kg, mixed-NT 8 g/kg, H. pluvialis 4 g/kg, AA 5 g/kg, and YEP 10 g/kg may present a novel strategy for improving reproduction performance and antioxidant activities in RC-crayfish, C. quadricarinatus by the positive impact on the molecular level of reproduction and antioxidant genes.

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Data availability

The data that support the findings of this study are available on request from the corresponding author. The data are not publicly available due to privacy or ethical constraints.

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Funding

This work was supported by the Startup Fund for Dr. Ziping Zhang (No. 104–61201403610) and Special Fund for Adjusting Marine and Fisheries Structure of Fujian Province (2019MDS-YT006).

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Authors and Affiliations

Authors

Contributions

Akram Ismael Shehata: conceptualization, methodology, validation, writing—original draft. Mayada Alhoshy: writing. Tao Wang: conceptualization, methodology, validation. Jianfeng Wang: helped supervise the project, provided crayfish and equipment. Ruoxuan Wang: collecting samples. Mahmoud A.O. Dawood: Mohamed A.A. Zaki: formal analysis, writing—review and editing. Yilei Wang: the original idea—review and editing. Ziping Zhang: original idea, writing—review and editing, Supervision. All authors contributed to preparing the final manuscript.

Corresponding authors

Correspondence to Jianfeng Wang or Ziping Zhang.

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The authors declare no competing interests.

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All of the study design and animal experiments were conducted in accordance with the guidelines of Fujian Agriculture and Forestry University’s Animal Care and Use Committee.

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Handling Editor: Gavin Burnell

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Shehata, A.I., Alhoshy, M., Wang, T. et al. Expression of reproduction and antioxidant-related genes in crayfish Cherax quadricarinatus fed with dietary feed additives. Aquacult Int 30, 699–720 (2022). https://doi.org/10.1007/s10499-021-00816-0

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  • DOI: https://doi.org/10.1007/s10499-021-00816-0

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