Abstract
The present study evaluated the effect of probiotic Lactobacillus sp. against pathogenic bacteria Vibrio harveyi in freshwater prawn Macrobrachium rosenbergii. The bacteria were isolated from the gut of prawn and identified using biochemical and PCR tests. Inoculation of probiotic (9 log CFU/g) and pathogenic bacteria (5 log CFU/g) mixture was employed for in vitro test. The in vivo challenge test was conducted in glass aquaria (45 × 30 × 30 cm3) and also in an earthen pond (70 m2, 1 m depth) for 8 weeks, individually. In both cases, only the treatment group was provided probiotic (9 log CFU/g) incorporated feed. Growth, digestibility, and immune response of prawn were determined. Both in the in vitro and in vivo test, V. harveyi growth was found to be reduced significantly at 8 hr (from 5 to 4 log CFU/g) and after 50 days (from 7 to 6 log CFU/g) of probiotic administration, respectively. In the pond, higher growth and better digestibility were found in the treatment group (WG%, 549. 30 ± 40.02%; SGR%, 3.12 ± 0.10%; amylase, 1.23 unit/mg; protease, 2.47 unit/mg) compared to the control group (WG%, 310.77 ± 18.68%; SGR%, 2. 35 ± 0.08%; 0.7 unit/mg; protease, 1.82 unit/mg). The immune response was found to be significantly different (P < 0.05) only for SGH (control, 22.76 ± 5.54%; probiotic, 19.32 ± 5.13%) and NGH (control, 75.12 ± 17.12%; probiotic, 78.12 ± 10.11%). Overall, the probiotic bacteria enhanced weight gain, digestibility, and immune response in the experimental prawn. Therefore, Lactobacillus sp. could be used as a potential probiotic for prawn culture against bacterial diseases.
Similar content being viewed by others
References
Ahmed N, Demaine H, Muir JF (2008) Freshwater prawn farming in Bangladesh: history, present status and future prospects. Aquac Res 39:806–819
Ahmmed MK, Ahmmed F, Kabir KA, Faisal M, Ahmed SI, Ahsan MN (2017) Biochemical impacts of salinity on the catfish, (Bloch, 1794), and possibility of their farming at low saline water. Aquac Res 48(8):4251–4261
Ahmmed F, Ahmmed MK, Shah MS, Banu GR (2018) Use of indigenous beneficial bacteria (Lactobacillus spp.) as probiotics in shrimp (Penaeus monodon) aquaculture. Res Agric Livestock Fish 5:127–135
Ahmmed MK, Ahmmed F, Tian HS, Carne A, Bekhit AE‐D (2020) Marine omega‐3 (n‐3) phospholipids: A comprehensive review of their properties, sources, bioavailability, and relation to brain health. Compr Rev Food Sci Food Saf 19(1):64–123
Ajitha S, Sridhar M, Sridhar N, Bright Singh I, Varghese V (2004) Probiotic effects of lactic acid bacteria against Vibrio alginolyticus in Penaeus (Fenneropenaeus) indicus (H. Milne Edwards). Asian Fish Sci 17:71–80
Anson ML (1938) The estimation of pepsin, trypsin, papain, and cathepsin with hemoglobin. J General Physiol 22:79–89
Asaduzzaman M, Wahab M, Verdegem M, Huque S, Salam M, Azim M (2008) C/N ratio control and substrate addition for periphyton development jointly enhance freshwater prawn Macrobrachium rosenbergii production in ponds. Aquaculture 280:117–123
Azad MAK, Islam SS, Sithi IN, Ghosh AK, Banu GR, Bir J, Huq KA (2019) Effect of probiotics on immune competence of giant freshwater prawn Macrobrachium rosenbergii. Aquac Res 50:644–657
Banerjee SK, Farber JM (2017) Detection, enumeration, and isolation of Vibrio parahaemolyticus and V. vulnificus from seafood: development of a multidisciplinary protocol. J AOAC Int 100:445–453
Brown K, DeCoffe D, Molcan E, Gibson DL (2012) Diet-induced dysbiosis of the intestinal microbiota and the effects on immunity and disease. Nutrients 4:1095–1119
Cowan ST (2004) Cowan and Steel's manual for the identification of medical bacteria. Cambridge university press
Dubernet S, Desmasures N, Guéguen M (2002) A PCR-based method for identification of Lactobacilli at the genus level. FEMS Microbiol Lett 214:271–275
Ferdose A, Hossain MB (2011) Nutritional value of wild, cultured and frozen prawn Macrobrachium rosenbergii (De Man, 1879). Int J Nat Sci 1(2):52–55
Haddaji N, Krifi B, Lagha R, Khouadja S, Bakhrouf A (2015) Effect of high temperature on viability of Lactobacillus casei and analysis of secreted and GroEL proteins profiles. Afr J Microbiol Res 7:29–35
Hannan MA, Rahman MM, Mondal MN, Deb SC, Chowdhury G, Islam MT (2019) Molecular identification of Vibrio alginolyticus causing Vibriosis in shrimp and its herbal remedy polish. J Microbiol 68:429–438
Hassanzadazar H, Ehsani A, Mardani K, Hesari J (2012) Investigation of antibacterial, acid and bile tolerance properties of Lactobacilli isolated from Koozeh cheese. In: veterinary research forum, 2012. Faculty of Veterinary Medicine, Urmia University, Urmia, Iran 3:181–185
Islam MR, Tabeta S (2019) Shrimp vs prawn-rice farming in Bangladesh: a comparative impacts study on local environments and livelihoods. Ocean Coast Manag 168:167–176
Khan S, Mahmud S (2019) The impact of probiotic bacterium Lactobacillus acidophilus in growth and survival of the juvenile freshwater river prawn (Macrobrachium rosenbergii) infected with pathogenic Vibrio sp. J Microbiol Biotechnol Food Sci 2019:225–229
Kongnum K, Hongpattarakere T (2012) Effect of Lactobacillus plantarum isolated from digestive tract of wild shrimp on growth and survival of white shrimp (Litopenaeus vannamei) challenged with Vibrio harveyi. Fish Shellfish Immunol 32:170–177
Kumar PNJ, Jyothsna R, Reddy MH, Sreevani S (2013) Effect of Bacillus subtilis and Lactobacillus rhamnosus incorporated probiotic diet on growth pattern and enzymes in Penaeus vannamei. Int J Life Sci Pharma Res 3:6–11
Levene H (1960) Contributions to probability and statistics. Essays in honor of Harold Hotelling:278–292
Li S, Yang H, Wang D, Lu T (2013) Measurement of concentration of bacterium Aeromonas hydrophila by UV spectrophotometry. Chin J Fish 26:40–42
Li X, Ringø E, Hoseinifar SH, Lauzon HL, Birkbeck H, Yang D (2019a) The adherence and colonization of microorganisms in fish gastrointestinal tract. Rev Aquac 11:603–618
Li X, Zhou Y, Jiang Q, Yang H, Pi D, Liu X, Gao X, Chen N, Zhang X (2019b) Virulence properties of Vibrio vulnificus isolated from diseased zoea of freshness shrimp Macrobrachium rosenbergii. Microb Pathog 127:166–171
Loftsson T, Ilievska B, Asgrimsdottir GM, Ormarsson OT, Stefansson E (2016) Fatty acids from marine lipids: biological activity, formulation and stability. J Drug Delivery Sci Technol 34:71–75
Lovett DL, Felder DL (1990) Ontogenetic change in digestive enzyme activity of larval and postlarval white shrimp Penaeus setiferus (Crustacea, Decapoda, Penaeidae). Biol Bull 178:144–159
Minitab I (2014) MINITAB release 17: statistical software for windows Minitab Inc, USA
Mujeeb Rahiman K, Jesmi Y, Thomas AP, Mohamed Hatha A (2010) Probiotic effect of Bacillus NL110 and Vibrio NE17 on the survival, growth performance and immune response of Macrobrachium rosenbergii (de Man). Aquac Res 41:120–134
Nandi A, Rout S, Dasgupta A, Abraham TJ (2009) Water quality characteristics in controlled production of ornamental fishes as influenced by feeding a probiotic bacterium, Lactobacillus sp bioencapsulated in Artemia sp. Indian J Fish 56:283–286
Rahman M, Hossain M (2009) Production and export of shrimp of Bangladesh: problems and prospects. Progress Agric 20:163–171
Rahman S, Khan SN, Naser MN, Karim MM (2009) Application of probiotic bacteria: a novel approach towards ensuring food safety in shrimp aquaculture. J Bangladesh Acad Sci 33:139–144
Ravan S, Mehrabadi M, Bandani A (2009) Biochemical characterization of digestive amylase of wheat bug, Eurygaster maura (Hemiptera: Scutelleridae). Afr J Biotechnol 8:3640–3648
Rico A, Phu T, Satapornvanit K, Min J, Shahabuddin AM, Henriksson PJG et al (2013) Use of veterinary medicines, feed additives and probiotics in four major internationally traded aquaculture species farmed in Asia. Aquaculture 412:231–243
Rossi LT, Sharpen AR, Zimmermann JA, Olivero CR, Zbrun MV et al (2020) Intestinal microbiota modulation in juvenile Pacú (Piaractus mesopotamicus) by supplementation with Pyropia columbina and β-carotene. Aquac Int:1–16
Safari R, Adel M, Lazado CC, Caipang CMA, Dadar M (2016) Host-derived probiotics Enterococcus casseliflavus improves resistance against Streptococcus iniae infection in rainbow trout (Oncorhynchus mykiss) via immunomodulation. Fish Shellfish Immunol 52:198–205
Sarter S, Nguyen HNK, Hung LT, Lazard J, Montet D (2007) Antibiotic resistance in gram-negative bacteria isolated from farmed catfish. Food Control 18:1391–1396
Seenivasan C, Bhavan PS, Radhakrishnan S, Shanthi R (2012) Enrichment of Artemia nauplii with Lactobacillus sporogenes for enhancing the survival, growth and levels of biochemical constituents in the post-larvae of the freshwater prawn Macrobrachium rosenbergii. Turk J Fish Aquat Sci 12:23–31
Sha Y, Wang B, Liu M, Jiang K, Wang L (2016) Interaction between Lactobacillus pentosus HC-2 and Vibrio parahaemolyticus E1 in Litopenaeus vannamei in-vivo and in-vitro. Aquaculture 465:117–123
Shapiro SS, Wilk MB (1965) An analysis of variance test for normality (complete samples). Biometrika 52:591–611
Sireswar S, Dey G, Sreesoundarya T, Sarkar D (2017) Design of probiotic-fortified food matrices influence their antipathogenic potential. Food Biosci 20:28–35
Sivakumar N, Sundararaman M, Selvakumar G (2012) Probiotic effect of Lactobacillus acidophilus against vibriosis in juvenile shrimp (Penaeus monodon). Afr J Biotechnol 11:15811–15818
Söderhäll K, Smith VJ (1983) Separation of the haemocyte populations of Carcinusmaenas and other marine decapods, and prophenoloxidase distribution. Dev Comp Immunol 7:229–239
Sumon MS, Ahmmed F, Khushi SS, Ahmmed MK, Rouf MA, Chisty MAH, Sarower MG (2018) Growth performance, digestive enzyme activity and immune response of Macrobrachium rosenbergii fed with probiotic Clostridium butyricum incorporated diets. J King Saud Univ Sci 30:21–28
Tarr CL, Patel JS, Puhr ND, Sowers EG, Bopp CA, Strockbine NA (2007) Identification of Vibrio isolates by a multiplex PCR assay and rpoB sequence determination. J Clin Microbiol 45:134–140
Torky HA, Abdellrazeq GS, Hussein MM, Ghanem NH (2016) Molecular characterization of Vibrio Harveyi in diseased shrimp. Alexandria J Vet Sci 37:523–532
Tukey JW (1949) Comparing individual means in the analysis of variance. Biometrics 5:99–114
Uberschar B (1993) Measurement of proteolytic enzyme activity: significance and application in larval fish research. Physiol Biochem Aspects Fish Dev:233–239
Uddin SA, Kader MA (2006) The use of antibiotics in shrimp hatcheries in Bangladesh. J Fish Aquat Sci 1:64–67
Vanderpool C, Yan F, Polk BD (2008) Mechanisms of probiotic action: implications for therapeutic applications in inflammatory bowel diseases. Inflamm Bowel Dis 14:1585–1596
Venkat HK, Sahu NP, Jain KK (2004) Effect of feeding Lactobacillus-based probiotics on the gut microflora, growth and survival of postlarvae of Macrobrachium rosenbergii (de Man). Aquac Res 35:501–507
Verschuere L, Rombaut G, Sorgeloos P, Verstraete W (2000) Probiotic bacteria as biological control agents in aquaculture. Microbiol Mol Biol Rev 64:655–671
Vine N, Leukes W, Kaiser H, Daya S, Baxter J, Hecht T (2004) Competition for attachment of aquaculture candidate probiotic and pathogenic bacteria on fish intestinal mucus. J Fish Dis 27:319–326
Wang Y-B (2007) Effect of probiotics on growth performance and digestive enzyme activity of the shrimp Penaeus vannamei. Aquaculture 269:259–264
Xu G, Xing W, Li T, Ma Z, Liu C, Jiang N, Luo L (2018) Effects of dietary raffinose on growth, non-specific immunity, intestinal morphology and microbiome of juvenile hybrid sturgeon (Acipenser baeri Brandt♀× A. schrenckii Brandt♂). Fish Shellfish Immunol 72:237–246
Ziaei-Nejad S, Rezaei MH, Takami GA, Lovett DL, Mirvaghefi AR, Shakouri M (2006) The effect of Bacillus spp. bacteria used as probiotics on digestive enzyme activity, survival and growth in the Indian white shrimp Fenneropenaeus indicus. Aquaculture 252:516–524
Acknowledgments
The authors would also like to acknowledge all members who helped in the collection of the juvenile prawns and preparation of the pond.
Funding
This project was funded by the Bangladesh Fisheries Research Institute (BFRI, grant no. BFRI/IDRS/RCN/2013-14/76(18)).
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Conflict of interest
The authors declare that they have no conflict of interest.
Ethical approval
Experiments on animals were performed under the guidelines and regulations of the “Biosafety and Ethics Committee,” Fisheries and Marine Resources Technology (FMRT) Discipline, Khulna University, Bangladesh.
Additional information
Publisher’s note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Rights and permissions
About this article
Cite this article
Ahmmed, F., Ahmmed, M.K., Khushi, S.S. et al. Host gut-derived probiotic Lactobacillus sp. improves resistance of giant freshwater prawn Macrobrachium rosenbergii against Vibrio harveyi. Aquacult Int 28, 1709–1724 (2020). https://doi.org/10.1007/s10499-020-00551-y
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10499-020-00551-y