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Study of sex determination system in ship sturgeon, Acipenser nudiventris using meiotic gynogenesis

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Abstract

The sex ratios and sex determination mechanism of gynogenetic diploids of ship sturgeon, Acipenser nudiventris, have been investigated to verify the possibilities of sex control by chromosome manipulation in this species. Meiotic gynogenesis was induced in a female ship sturgeon using cold shock after egg activation with UV-irradiated sperm of a male Siberian sturgeon. Microsatellite DNA analysis was applied for verification of uniparental inheritance in the gynogenetic diploid group of fish. All the analyzed gynogenetic diploids possessed only maternal genotype in the examined experimental group of fish. In this study, a minimum of two distinctly selected diagnostic loci in the offspring was used to confirm exclusively maternal contribution. Also, these fish were analyzed for sex diagnostic. Histological analysis of gonads from gynogenetic diploids, obtained from one family, showed 73.3 % of females and 27.7 % of males. The observed sex ratio has suggested that the ship sturgeon have a female heterogametic sex determination system. Gynogenesis in this species with female heterogametic sex determination system will have important role in breeding program and reclamation of its natural population to produce both female and male progeny, while this species has been introduced in the red list of IUCN (International Union for Conservation of Nature and Natural Resources).

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Acknowledgments

This study was financially supported by the Iranian Fisheries Research Organization. I thank from head of Biochemistry & Physiology for his heart collaboration on fish gonadectomy for the study.

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Authors and Affiliations

  1. International Sturgeon Research Institute, P.O. Box 41635-3464, Rasht, Iran

    Mohammad Hassanzadeh Saber & Ali Hallajian

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  1. Mohammad Hassanzadeh Saber
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  2. Ali Hallajian
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Correspondence to Mohammad Hassanzadeh Saber.

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Hassanzadeh Saber, M., Hallajian, A. Study of sex determination system in ship sturgeon, Acipenser nudiventris using meiotic gynogenesis. Aquacult Int 22, 273–279 (2014). https://doi.org/10.1007/s10499-013-9676-z

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  • DOI: https://doi.org/10.1007/s10499-013-9676-z

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