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Drosophila jumu modulates apoptosis via a JNK-dependent pathway and is required for other processes in wing development

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Abstract

Previous studies in several model organisms have revealed that members of the Forkhead (Fkh) transcription factor family have multiple functions. Drosophila Jumeau (Jumu), a member of this family, participates in cardiogenesis, hematopoiesis and immune system homeostasis. Here, we show that loss of jumu function positively regulates or triggers apoptosis via a JNK-dependent pathway in wing development. jumu mutants showed reduced wing size and increased apoptosis. Moreover, we observed a loss of the anterior cross vein (ACV) phenotype that was similar to that observed in wings in which JNK signaling has been ectopically activated. The JNK signaling markers puckered (puc) and p-JNK were also significantly increased in the wing discs of jumu mutants. In addition, apoptosis induced by the loss of jumu was rescued by knocking down JNK, indicating a role for JNK in reducing jumu-induced apoptosis. Jumu could also control wing margin development via the positive regulation of cut expression, and the observed wing margin defect did not result from a loss of jumu-induced apoptosis. Further, jumu deficiency in the pupal wing could induce multiple wing hairs via a Rho1-mediated planar cell polarity pathway, but abnormal Rho1 expression was not why jumu loss induced apoptosis via a JNK-dependent pathway in wing discs.

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Acknowledgements

We thank Alan M Michelson for supplying us with the fly strains used in this study. We gratefully acknowledge Vienna Drosophila RNAi Stock Center, Tsinghua Drosophila model animal center, GenExel Stock Center and Developmental Studies Hybridoma Bank for providing fly lines and antibodies.

Funding

This work was supported by the National Natural Science Foundation of China (31772521) and Fundamental Research Funds for the Central Universities (2572018CG05, 2572015AA10).

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Authors and Affiliations

  1. Department of Genetics, College of Life Sciences, Northeast Forestry University, Harbin, 150040, China

    Xiao Chun Wang & Li Hua Jin

  2. Heilongjiang Academy of Agricultural Sciences, Harbin, 150040, China

    Ziguang Liu

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  1. Xiao Chun Wang
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  2. Ziguang Liu
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XCW, investigation, visualization, writing—original draft; LZ, review; LHJ, supervision, funding acquisition, project administration—review and editing.

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Correspondence to Li Hua Jin.

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Wang, X.C., Liu, Z. & Jin, L.H. Drosophila jumu modulates apoptosis via a JNK-dependent pathway and is required for other processes in wing development. Apoptosis 24, 465–477 (2019). https://doi.org/10.1007/s10495-019-01527-x

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