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Rybp interacts with Hippi and enhances Hippi-mediated apoptosis

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Abstract

Rybp (DEDAF) has been shown to interact with DED-containing proteins and to encode pro-apoptotic functions. Here we characterize a novel interaction between Rybp and Hippi, a protein implicated in neuronal apoptosis as well as in the pathogenesis of Huntington’s disease. Rybp can synergize with Hippi to enhance Caspase 8-mediated apoptosis and also appears to be essential for Hippi-mediated apoptosis. Moreover, Rybp may mediate or regulate the interaction between Hippi and Caspase 8. Finally, Rybp and Hippi co-localize in a subset of neurons in the developing mouse brain. Together, these findings suggest that Rybp and Hippi may functionally interact in the apoptotic processes that accompany normal murine neural development.

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Abbreviations

AS:

Antisense

CT:

Carboxyl terminus

DED:

Death effector domain

DEDAF:

Death effector domain associated factor

FADD:

Fas associated protein with Death Domain

Hip1:

Huntingtin interacting protein 1

Hippi:

Hip1 protein interactor

MLD:

Myosin like domain

NT:

Amino terminus

NZF:

Npl4 zinc finger

pDED:

Pseudo-death effector domain

Rybp:

Ring1 and YY1 binding protein

Yaf2:

YY1 activating factor 2

YY1:

Ying Yang 1

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Acknowledgments

We thank the members of the Schreiber-Agus laboratory for their thoughtful insight, comments on this work, and for providing unpublished plasmid constructs. We also thank Lisa McReynolds and Todd Evans for help with the Acridine Orange/Ethidium Bromide assay microscopy, and Harry Hou for providing the newborn mice. The sectioning and staining for the immunohistochemistry was performed by Dr. Radma Mahmood and the Histopathology Shared Resource Center of the Albert Einstein Cancer Center. Data in this paper are from a thesis (SES) submitted in partial fulfillment of the requirements for the Degree of Doctor of Philosophy in the Graduate Division of Medical Sciences, Albert Einstein College of Medicine, Yeshiva University. This work was supported in part by NIH Public Health Service grants CA92558 (NSA), CA68440 (JL), CA104292 (JL), NIH Medical Scientist Training Program Grant T32GM07288 (SES), and the Albert Einstein Cancer Center (NSA and JL).

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Authors and Affiliations

  1. Department of Molecular Genetics, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Ullmann 809, Bronx, NY, 10461, USA

    Sasha E. Stanton, Jennifer K. Blanck & Nicole Schreiber-Agus

  2. Department of Pathology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY, 10461, USA

    Joseph Locker

Authors
  1. Sasha E. Stanton
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  2. Jennifer K. Blanck
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  3. Joseph Locker
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  4. Nicole Schreiber-Agus
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Correspondence to Sasha E. Stanton or Nicole Schreiber-Agus.

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Stanton, S.E., Blanck, J.K., Locker, J. et al. Rybp interacts with Hippi and enhances Hippi-mediated apoptosis. Apoptosis 12, 2197–2206 (2007). https://doi.org/10.1007/s10495-007-0131-3

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