Abstract
Hyalomma asiaticum and H. anatolicum are tick species in Eurasia and Africa with major medical and veterinary significance. Beside their direct pathogenic effects, H. asiaticum and H. anatolicum are vectors of important diseases of livestock and in some instances of zoonoses. In search of ways to address the increasing incidence of global acaricide resistance, tick control through vaccination is regarded as a sustainable alternative approach. Cathepsin L-like cysteine protease (CPL) is a potent hemoglobinase, and plays important roles in the digestion of blood acquired from a host. CPL from H. anatolicum (HanCPL) with high similarity (> 90%) for H. asiaticum CPL (HasCPL) were aligned by in silico analysis. After further in vitro validation, the anti-HasCPL sera have cross-reactivity between the different total native protein of life stages and tissues for H. asiaticum and H. anatolicum. Furthermore, we further confirmed that recombinant HasCPL (rHasCPL) immunized rabbits were partially cross-protected (54.8%) by H. anatolicum infestation.
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This work was supported by University-level Independently Fund Scientific Research Projects of Shihezi University (ZZZC202130) and National Natural Science Foundation of China (31660711). The funder had no role in the study design, data collection and analysis, decision to publish or preparation of the manuscript.
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RS and CB designed this study, analyzed the results, drafted and edited the manuscript; RS, XZ, and XF collected samples and recorded samples’ data; RS edited the manuscript; RS, XZ, and TG did statistical analysis of the results, developed the project and the study protocol. All authors read and approved the final version of the manuscript.
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Song, R., Zhai, X., Fan, X. et al. Prediction and validation of cross-protective candidate antigen of Hyalomma asiaticum cathepsin L between H. asiaticum and H. anatolicum. Exp Appl Acarol 86, 283–298 (2022). https://doi.org/10.1007/s10493-022-00689-9
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DOI: https://doi.org/10.1007/s10493-022-00689-9