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Toxicity, repellency and anti-cholinesterase activities of thymol-eucalyptus combinations against phenotypically resistant Rhipicephalus annulatus ticks

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Abstract

The current study was designed to compare the acaricidal activity of pure thymol (T) crystals, eucalyptus oil (E), and their combinations (T + E) at concentrations ranging from 0.625 to 10% against phenotypically deltamethrin-resistant Rhipicephalus annulatus ticks. The adulticidal, larvicidal, ovicidal, and repellent activities of the tested compounds and their possible mechanism of action were studied. Thymol showed 93.3% adulticidal activity at 10% concentration, whereas at 2.5% it achieved 100% larvicidal activity. Eucalyptus oil had a significant effect on tick adults at concentrations > 5%, and at 10% concentration it showed 96.7% adulticidal activity and stopped the egg hatching. On deltamethrin-resistant larvae, the median lethal concentration (LD50) was 0.61, 7.5, and 0.61% for T, E, and T + E combinations, respectively, and repellent activities were 85.7, 62.5, and 100% at the 10% concentration used. Combinations of T + E did not induce additional effects as adulticidal and larvicidal in a comparison with T treatments. The tick’s acetylcholinesterase significantly decreased as a result of all treatments including deltamethrin, whereas lipid peroxidation significantly increased compared with the control untreated ticks. The glutathione content significantly decreased as a result of all treatments except for treatments with 10% thymol and its combination with eucalyptus oil. Moreover, 10% eucalyptus oil significantly decreased the tick’s glutathione peroxidase and catalase activities reflecting the attenuation of antioxidant defense in the ticks. In conclusion, thymol and eucalyptus oil showed excellent activities against deltamethrin-resistant R. annulatus isolates. Their action may be mediated via acetylcholinesterase inhibition. The effect of thymol-eucalyptus combinations recorded higher activities as an ovicidal and as a repellent.

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References

  • Abbas RZ, Zaman MA, Colwell DD, Gilleard J, Iqbal Z (2014) Acaricide resistance in cattle ticks and approaches to its management: the state of play. Vet Parasitol 203(1–2):6–20

    Article  CAS  PubMed  Google Scholar 

  • Abdelgaleil SA, Badawy ME, Mahmoud NF, Marei AE-SM (2019) Acaricidal activity, biochemical effects and molecular docking of some monoterpenes against two-spotted spider mite (Tetranychus urticae Koch). Pestic Biochem Physiol 156:105–115

    Article  CAS  PubMed  Google Scholar 

  • Aboelhadid SM, Arafa WM, Mahrous LN, Fahmy MM, Kamel AA (2018a) Molecular detection of Rhipicephalus (Boophilus) annulatus resistance against deltamethrin in middle Egypt. Vet Parasitol Reg Stud Rep 13:198–204. https://doi.org/10.1016/j.vprsr.2018.06.008

    Article  CAS  Google Scholar 

  • Aboelhadid SM, Arafa WM, Wahba A, Mahrous LN, Ibrahium SM, Holman PJ (2018b) Effect of high concentrations of lufenuron, pyriproxyfen and hydroprene on Rhipicephalus (Boophilus) annulatus. Vet parasitol 256:35–42

    Article  CAS  PubMed  Google Scholar 

  • Agus HH, Sengoz CO, Yilmaz S (2019) Oxidative stress-mediated apoptotic cell death induced by camphor in sod1-deficient Schizosaccharomyces pombe. Toxicol Res 8(2):216–226

    Article  CAS  Google Scholar 

  • Arafa WM, Mohammed AN, El-Ela FIA (2019) Acaricidal efficacy of deltamethrin-zinc oxide nanocomposite on Rhipicephalus (Boophilus) annulatus tick. Vet parasitol 268:36–45

    Article  CAS  PubMed  Google Scholar 

  • Araújo L, Novato T, Zeringota V, Maturano R, Melo D, Da Silva B, Daemon E, De Carvalho M, Monteiro C (2016) Synergism of thymol, carvacrol and eugenol in larvae of the cattle tick, Rhipicephalus microplus, and brown dog tick, Rhipicephalus sanguineus. Med Vet Entomol 30(4):377–382

    Article  PubMed  Google Scholar 

  • Barimani A, Youssefi MR, Tabari MA (2016) Traps containing carvacrol, a biological approach for the control of Dermanyssus gallinae. Parasitol Res 115(9):3493–3498

    Article  PubMed  Google Scholar 

  • Benelli G, Pavela R, Canale A, Mehlhorn H (2016) Tick repellents and acaricides of botanical origin: a green roadmap to control tick-borne diseases? Parasitol Res 115(7):2545–2560

    Article  PubMed  Google Scholar 

  • Beutler E (1963) Improved method for the determination of blood glutathione. J lab Clin Med 61:882–888

    CAS  PubMed  Google Scholar 

  • Bossou AD, Ahoussi E, Ruysbergh E, Adams A, Smagghe G, De Kimpe N, Avlessi F, Sohounhloue DC, Mangelinckx S (2015) Characterization of volatile compounds from three Cymbopogon species and Eucalyptus citriodora from Benin and their insecticidal activities against Tribolium castaneum. Ind Crops Prod 76:306–317

    Article  CAS  Google Scholar 

  • Bradberry SM, Cage SA, Proudfoot AT, Vale JA (2005) Poisoning due to pyrethroids. Toxicol Rev 24(2):93–106

    Article  CAS  PubMed  Google Scholar 

  • Brooker M, Kleinig D (2006) Field guide to eucalyptus. Inkata Press, Melbourne

    Google Scholar 

  • Camargo-Mathias M (2018) Inside ticks: morphophysiology, toxicology and therapeutic perspectives. Editora UNESP, São Paulo

    Google Scholar 

  • Camargo-Mathias MI, Pereira NRC, da Silva Reis C, de Almeida CR, dos Santos Mendes DR, de Araújo GB, Postali L, Figueroa T, Ferreira ARF, Santos JP (2017) Deltamethrin as inductor agent of precocious ovarian degeneration in Rhipicephalus sanguineus sl (Acari: Ixodidae) ticks. Exp Appl Acarol 72(2):161–169

    Article  CAS  PubMed  Google Scholar 

  • Chagas ACdS, Passos WM, Prates HT, Leite RC, Furlong J, Fortes ICP (2002) Acaricide effect of Eucalyptus spp essential oils and concentrated emulsion on Boophilus microplus. Braz J Vet Res Anim Sci 39(5):247–253

    Article  Google Scholar 

  • Chansang A, Champakaew D, Junkum A, Jitpakdi A, Amornlerdpison D, Aldred AK, Riyong D, Wannasan A, Intirach J, Muangmoon R (2018) Synergy in the adulticidal efficacy of essential oils for the improvement of permethrin toxicity against Aedes aegypti L. (Diptera: Culicidae). Parasit Vectors 11(1):417

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Cimanga K, Kambu K, Tona L, Apers S, De Bruyne T, Hermans N, Totté J, Pieters L, Vlietinck AJ (2002) Correlation between chemical composition and antibacterial activity of essential oils of some aromatic medicinal plants growing in the Democratic Republic of Congo. J Ethnopharmacol 79(2):213–220

    Article  CAS  PubMed  Google Scholar 

  • Clemente MA, de Oliveira Monteiro CM, Scoralik MG, Gomes FT, de Azevedo Prata MC, Daemon E (2010) Acaricidal activity of the essential oils from Eucalyptus citriodora and Cymbopogon nardus on larvae of Amblyomma cajennense (Acari: Ixodidae) and Anocentor nitens (Acari: Ixodidae). Parasitol Res 107(4):987–992

    Article  PubMed  Google Scholar 

  • Cohen G, Dembiec D, Marcus J (1970) Measurement of catalase activity in tissue extracts. Anal Biochem 34(1):30–38

    Article  CAS  PubMed  Google Scholar 

  • Costa-Júnior LM, Miller RJ, Alves PB, Blank AF, Li AY, de León AAP (2016) Acaricidal efficacies of Lippia gracilis essential oil and its phytochemicals against organophosphate-resistant and susceptible strains of Rhipicephalus (Boophilus) microplus. Vet Parasitol 228:60–64

    Article  PubMed  CAS  Google Scholar 

  • da Silva Mendes A, Daemon E, de Oliveira Monteiro CM, Maturano R, Brito FC, Massoni T (2011) Acaricidal activity of thymol on larvae and nymphs of Amblyomma cajennense (Acari: Ixodidae). Vet Parasitol 183(1–2):136–139

    Article  CAS  Google Scholar 

  • Daemon E, de Oliveira Monteiro CM, dos Santos Rosa L, Clemente MA, Arcoverde A (2009) Evaluation of the acaricide activity of thymol on engorged and unengorged larvae of Rhipicephalus sanguineus (Latreille, 1808 ) (Acari: Ixodidae). Parasitol Res 105(2):495

    Article  PubMed  Google Scholar 

  • Daemon E, Maturano R, de Oliveira Monteiro CM, Goldner MS, Massoni T (2012) Acaricidal activity of hydroethanolic formulations of thymol against Rhipicephalus sanguineus (Acari: Ixodidae) and Dermacentor nitens (Acari: Ixodidae) larvae. Vet Parasitol 186(3–4):542–545

    Article  CAS  PubMed  Google Scholar 

  • de Oliveira Monteiro CM, Daemon E, Clemente MA, dos Santos Rosa L, Maturano R (2009) Acaricidal efficacy of thymol on engorged nymphs and females of Rhipicephalus sanguineus (Latreille, 1808)(Acari: Ixodidae). Parasitol Res 105(4):1093

    Article  PubMed  Google Scholar 

  • de Oliveira Monteiro CM, Daemon E, Silva AMR, Maturano R, Amaral C (2010) Acaricide and ovicide activities of thymol on engorged females and eggs of Rhipicephalus (Boophilus) microplus (Acari: Ixodidae). Parasitol Res 106(3):615–619

    Article  PubMed  Google Scholar 

  • de Monteiro CM, Maturano R, Daemon E, Catunda-Junior FEA, Calmon F, de Souza Senra T, Faza A, de Carvalho MG (2012) Acaricidal activity of eugenol on Rhipicephalus microplus (Acari: Ixodidae) and Dermacentor nitens (Acari: Ixodidae) larvae. Parasitol Res 111(3):1295–1300

    Article  PubMed  Google Scholar 

  • Denardi SE, Bechara GH, de Oliveira PR, Camargo Mathias MI (2011) Inhibitory action of neem aqueous extract (Azadirachta indica A. Juss) on the vitellogenesis of Rhipicephalus sanguineus (Latreille, 1806) (Acari: Ixodidae) ticks. Microsc Res Tech 74(10):889–899

    Article  PubMed  Google Scholar 

  • Dhifi W, Bellili S, Jazi S, Bahloul N, Mnif W (2016) Essential oils’ chemical characterization and investigation of some biological activities: a critical review. Medicines 3(4):25

    Article  PubMed Central  CAS  Google Scholar 

  • Drummond R, Ernst S, Trevino J, Gladney W, Graham O (1973) Boophilus annulatus and B. microplus: laboratory tests of insecticides. J Econ Entomol 66(1):130–133

    Article  CAS  PubMed  Google Scholar 

  • Ellman GL, Courtney KD, Andres V Jr, Featherstone RM (1961) A new and rapid colorimetric determination of acetylcholinesterase activity. Biochem Pharmacol 7(2):88–95

    Article  CAS  PubMed  Google Scholar 

  • Estrada-Peña A (2004) Ticks of domestic animals in the Mediterranean region: a guide to identification of species. University of Zaragoza, Zaragoza

    Google Scholar 

  • Faraone N, Hillier NK, Cutler GC (2015) Plant essential oils synergize and antagonize toxicity of different conventional insecticides against Myzus persicae (Hemiptera: Aphididae). PLoS ONE 10(5):e0127774

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Gallardo A, Picollo MI, González-Audino P, Mougabure-Cueto G (2012) Insecticidal activity of individual and mixed monoterpenoids of geranium essential oil against Pediculus humanus capitis (Phthiraptera: Pediculidae). J Med Entomol 49(2):332–335

    Article  CAS  PubMed  Google Scholar 

  • Gardulf A, Wohlfart I, Gustafson R (2004) A prospective cross-over field trial shows protection of lemon eucalyptus extract against tick bites. J Med Entomol 41(6):1064–1067

    Article  PubMed  Google Scholar 

  • Goudoum A, Tinkeu L, Ngassoum M, Mbofung C (2012) Biodegradation of insecticidal compounds of Clausena anisata and Plectranthus glandulosus essential oils applied as protectant on stored grains. Agric J 7(2):165–171

    Article  Google Scholar 

  • Greive KA, Barnes TM (2018) The efficacy of Australian essential oils for the treatment of head lice infestation in children: a randomised controlled trial. Aust J Dermatol 59(2):e99–e105

    Article  Google Scholar 

  • Hinton HE (1982) Biology of insect egg shells. Pergamon, Oxford, p 12

    Google Scholar 

  • Iori A, Grazioli D, Gentile E, Marano G, Salvatore G (2005) Acaricidal properties of the essential oil of Melaleuca alternifolia Cheel (tea tree oil) against nymphs of Ixodes ricinus. Vet Parasitol 129(1–2):173–176

    Article  CAS  PubMed  Google Scholar 

  • Jukic M, Politeo O, Maksimovic M, Milos M, Milos M (2007) In vitro acetylcholinesterase inhibitory properties of thymol, carvacrol and their derivatives thymoquinone and thymohydroquinone. Phytother Res 21(3):259–261

    Article  CAS  PubMed  Google Scholar 

  • Kalyanasundaram M, Das P (1985) Larvicidal & synergestic activity of plant extracts for mosquito control. Indian J Med Res 82(1):19–23

    CAS  PubMed  Google Scholar 

  • Khater H, Hendawy N, Govindarajan M, Murugan K, Benelli G (2016) Photosensitizers in the fight against ticks: safranin as a novel photodynamic fluorescent acaricide to control the camel tick Hyalomma dromedarii (Ixodidae). Parasitol Res 115(10):3747–3758

    Article  PubMed  Google Scholar 

  • Koul O, Singh R, Kaur B, Kanda D (2013) Comparative study on the behavioral response and acute toxicity of some essential oil compounds and their binary mixtures to larvae of Helicoverpa armigera, Spodoptera litura and Chilo partellus. Ind Crops Prod 49:428–436

    Article  CAS  Google Scholar 

  • Matkovics B, Kotorman M, Varga IS, Hai DQ, Varga C (1997) Oxidative stress in experimental diabetes induced by streptozotocin. Acta Physiol Hung 85(1):29–38

    CAS  PubMed  Google Scholar 

  • da Matos RS, Daemon E, Camargo-Mathias MI, Furquim KCS, Sampieri BR, Remedio RN, Araujo LX, Novato TPL (2014a) Histopathological study of ovaries of Rhipicephalus sanguineus (Acari: Ixodidae) exposed to different thymol concentrations. Parasitol Res 113(12):4555–4565

    Article  Google Scholar 

  • Matos RS, Melo DR, Monteiro CMO, Zeringóta V, Senra TOS, Calmon F, Maturano R, Prata MCA, Daemon E (2014b) Determination of the susceptibility of unengorged larvae and engorged females of Rhipicephalus microplus (Acari: Ixodidae) to different methods of dissolving thymol. Parasitol Res 113(2):669–673

    Article  PubMed  Google Scholar 

  • Matos RS, Daemon E, de Oliveira Monteiro CM, Sampieri BR, Marchesini PBC, Delmonte C, Camargo-Mathias MI (2019) Thymol action on cells and tissues of the synganglia and salivary glands of Rhipicephalus sanguineus sensu lato females (Acari: Ixodidae). Ticks Tick Borne Dis 10(2):314–320

    Article  PubMed  Google Scholar 

  • Minjauw B, McLeod A (2003) The impact of ticks and tick-borne diseases on the livelihood of small scale and marginal livestock owners in India and eastern and southern Africa. Tick-borne diseases and poverty. Research report, DFID animal health programme, centre for tropical veterinary medicine. University of Edinburgh, Edinburgh

    Google Scholar 

  • Novato TPL, Araújo LX, de Monteiro CMO, Maturano R, Senra TdOS, da Silva Matos R, Gomes GA, de Carvalho MG, Daemon E (2015) Evaluation of the combined effect of thymol, carvacrol and (E)-cinnamaldehyde on Amblyomma sculptum (Acari: Ixodidae) and Dermacentor nitens (Acari: Ixodidae) larvae. Vet Parasitol 212(3–4):331–335

    Article  CAS  PubMed  Google Scholar 

  • Ntalli NG, Ferrari F, Giannakou I, Menkissoglu-Spiroudi U (2011) Synergistic and antagonistic interactions of terpenes against Meloidogyne incognita and the nematicidal activity of essential oils from seven plants indigenous to Greece. Pest Manag Sci 67(3):341–351

    Article  CAS  PubMed  Google Scholar 

  • Pirali-Kheirabadi K, Razzaghi-Abyaneh M, Halajian A (2009) Acaricidal effect of Pelargonium roseum and Eucalyptus globulus essential oils against adult stage of Rhipicephalus (Boophilus) annulatus in vitro. Vet Parasitol 162(3–4):346–349

    Article  CAS  PubMed  Google Scholar 

  • Preuss HG, Jarrell ST, Scheckenbach R, Lieberman S, Anderson RA (1998) Comparative effects of chromium, vanadium and Gymnema sylvestre on sugar-induced blood pressure elevations in SHR. J Am Coll Nutr 17(2):116–123

    Article  CAS  PubMed  Google Scholar 

  • Pruett J (1999) Immunological control of arthropod ectoparasites—a review. Int J Parasitol 29(1):25–32

    Article  CAS  PubMed  Google Scholar 

  • Rodríguez-Vivas RI, Hodgkinson JE, Trees AJ (2012) Resistencia a los acaricidas en Rhipicephalus (Boophilus) microplus: situación actual y mecanismos de resistencia. Rev Mex Cienc Pecu 3:9–24

    Google Scholar 

  • Rodriguez-Vivas RI, Jonsson NN, Bhushan C (2018) Strategies for the control of Rhipicephalus microplus ticks in a world of conventional acaricide and macrocyclic lactone resistance. Parasitol Res 117(1):3–29

    Article  PubMed  Google Scholar 

  • Sathantriphop S, Achee NL, Sanguanpong U, Chareonviriyaphap T (2015) The effects of plant essential oils on escape response and mortality rate of Aedes aegypti and Anopheles minimus. J Vector Ecol 40(2):318–326

    Article  PubMed  Google Scholar 

  • Scoralik MG, Daemon E, de Oliveira Monteiro CM, Maturano R (2012) Enhancing the acaricide effect of thymol on larvae of the cattle tick Rhipicephalus microplus (Acari: Ixodidae) by solubilization in ethanol. Parasitol Res 110(2):645–648

    Article  PubMed  Google Scholar 

  • Senra TdOS, Zeringóta V, de Oliveira Monteiro CM, Calmon F, Maturano R, Gomes GA, Faza A, de Carvalho MG, Daemon E (2013) Assessment of the acaricidal activity of carvacrol,(E)-cinnamaldehyde, trans-anethole, and linalool on larvae of Rhipicephalus microplus and Dermacentor nitens (Acari: Ixodidae). Parasitol Res 112(4):1461–1466

    Article  Google Scholar 

  • Sharma AK, Kumar R, Kumar S, Nagar G, Singh NK, Rawat SS, Dhakad M, Rawat A, Ray D, Ghosh S (2012) Deltamethrin and cypermethrin resistance status of Rhipicephalus (Boophilus) microplus collected from six agro-climatic regions of India. Vet Parasitol 188(3–4):337–345

    Article  CAS  PubMed  Google Scholar 

  • Slimane BB, Ezzine O, Dhahri S, Jamaa MLB (2014) Essential oils from two Eucalyptus from Tunisia and their insecticidal action on Orgyia trigotephras (Lepidotera, Lymantriidae). Biol Res 47(1):29

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Soleimani-Ahmadi M, Abtahi SM, Madani A, Paksa A, Abadi YS, Gorouhi MA, Sanei-Dehkordi A (2017) Phytochemical profile and mosquito larvicidal activity of the essential oil from aerial parts of Satureja bachtiarica Bunge against malaria and lymphatic filariasis vectors. J Essent Oil Bear Plant 20(2):328–336

    Article  CAS  Google Scholar 

  • Stone B, Haydock K (1962) A method for measuring the acaricide-susceptibility of the cattle tick Boophilus microplus (Can.). Bull Entomol Res 53(3):563–578

    Article  CAS  Google Scholar 

  • Tabari MA, Youssefi MR, Maggi F, Benelli G (2017) Toxic and repellent activity of selected monoterpenoids (thymol, carvacrol and linalool) against the castor bean tick, Ixodes ricinus (Acari: Ixodidae). Vet Parasitol 245:86–91

    Article  CAS  PubMed  Google Scholar 

  • Taktak NE, Badawy ME (2019) Potential of hydrocarbon and oxygenated monoterpenes against Culex pipiens larvae: toxicity, biochemical, pharmacophore modeling and molecular docking studies. Pestic Biochem Phys 158:156

    Article  CAS  Google Scholar 

  • Tong F, Bloomquist JR (2013) Plant essential oils affect the toxicities of carbaryl and permethrin against Aedes aegypti (Diptera: Culicidae). J Med Entomol 50(4):826–832

    Article  CAS  PubMed  Google Scholar 

  • Vivekanandhan P, Usha-Raja-Nanthini A, Valli G, Subramanian Shivakumar M (2018) Comparative efficacy of Eucalyptus globulus (Labill) hydrodistilled essential oil and temephos as mosquito larvicide. Nat Prod Res. https://doi.org/10.1080/14786419.2018.1547290

    Article  Google Scholar 

  • Wanzala W, Sika N, Gule S, Hassanali A (2004) Attractive and repellent host odours guide ticks to their respective feeding sites. Chemoecology 14(3–4):229–232

    Google Scholar 

  • Yang Y-C, Choi H-Y, Choi W-S, Clark J, Ahn Y-J (2004) Ovicidal and adulticidal activity of Eucalyptus globulus leaf oil terpenoids against Pediculus humanus capitis (Anoplura: Pediculidae). J Agric Food Chem 52(9):2507–2511

    Article  CAS  PubMed  Google Scholar 

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Acknowledgements

This work was covered through a project funded from Beni-Suef University. Authors appreciate veterinarians who helped during tick collection.

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Authors and Affiliations

  1. Department of Parasitology, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef, 62511, Egypt

    Waleed M. Arafa & Shawky M. Aboelhadid

  2. Pharmacognosy Department, Faculty of Pharmacy, Beni-Suef University, Beni-Suef, 62514, Egypt

    Abeer Moawad

  3. Egypt Animal Health Research Institute, Beni-Suef, Egypt

    Khalid M Shokeir

  4. Physiology Division, Zoology Department, Faculty of Sciences, Beni-Suef University, Beni-Suef, 62521, Egypt

    Osama Ahmed

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  1. Waleed M. Arafa
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Arafa, W.M., Aboelhadid, S.M., Moawad, A. et al. Toxicity, repellency and anti-cholinesterase activities of thymol-eucalyptus combinations against phenotypically resistant Rhipicephalus annulatus ticks. Exp Appl Acarol 81, 265–277 (2020). https://doi.org/10.1007/s10493-020-00506-1

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