Abstract
Dokdo islands in Republic of Korea, is not suited to survive plant because strong wind, low organic content and high salinity. Nevertheless, 64 taxa of plants have a symbiotic relationship with microbe. The Metabacillus elymi KUDC1714T was isolated from the rhizosphere of Elymus tsukushiensis collected from Dokdo Islands. This bacterial strain was Gram-stain positive, non-motile, non-spore forming, aerobic and rod-shape bacteria with 0.4–0.5 × 2.5–3.0 μm in size. Colonies were smooth, yellowish-white circular and 2.0–3.0 mm in diameter. KUDC1714T was capable of growing at 10–45 °C (optimum, 30 °C), pH 7–11 (optimum, pH 8) and 0–8.0% (w/v) NaCl (optimum, 1.0–2.0%). Flagella were not observed by transmission electron microscopy. Phylogenetic analyses, based on 16S rRNA gene sequences, showed that KUDC1714T belonged to the genus Metabacillus formed a phyletic lineage within the genus Metabacillus of the family Bacillaceae. The strain KUDC1714T was most closely related to Metabacillus sediminilitoris DSL-17 T (98.2%), Metabacillus litoralis SW-211 T (98.2%) and Metabacillus halosaccharovorans E33T (97.7%) based on 16S rRNA gene sequence. In silico DNA-DNA hybridization using genome-to-genome distance calculator were analysed at 25.8% and 23.5% in strain KUDC1714T and Metabacillus sediminilitoris DSL-17 T, and strain KUDC1714T and Metabacillus litoralis SW-211 T, respectively. Strain KUDC1714T and its closet type strain were all below cut-off point of the average nucleotide identity and average amino acid identity values. The genome contains 5197 CDSs, 3 rRNAs, 118 tRNAs, 5 ncRNAs. The genomic DNA G + C content was 34.8 mol%. Its polar lipids were diphosphatidylglycerol, phosphatidylglycerol and its major fatty acids were anteiso-C15:0 and iso-C15:0. The strain KUDC1714T contained menaquinone-7 as major isoprenoid quinone. Based on its genetic data, physiological and biochemical characteristics, strain KUDC1714T should be considered a novel species of the genus Metabacillus, for which we propose the name Metabacillus elymi sp. nov. the type strain is KUDC1714T (= KCTC 33222 T, = DSM 27608 T).
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Category: New Taxa (Firmicutes and Related Organisms). The GenBank/EMBL/DDBJ accession number for the partial 16S rRNA gene sequence of KUDC1714T is KC414705 and CP055263.
Abbreviations
- PG:
-
Phosphatidylglycerol
- DPG:
-
Diphosphatidylglycerol
- TSA:
-
Tryptic soy agar
- TSB:
-
Tryptic soy broth
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Acknowledgements
We would like to thank the Strategic Initiative for Microbiomes in Agriculture and Food, which is funded by the Ministry of Agriculture, Food and Rural Affairs (918010-4), and the Basic Science Research Program of the National Research Foundation of Korea (NRF), which is funded by the Ministry of Education (No. 2016R1A05011910), for technical support.
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This research was supported by the Basic Science Research Program of the National Research Foundation of Korea (NRF), funded by the Ministry of Education (No. 2016R1A05011910).
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S-Y, Lee and J-S, Son performed experiments and data collection. S-Y, Lee and J-S, Son wrote most of the paper. Y-J, Hwang offered data analysis and interpretation. J-H, shin carried out experiment of whole-genome sequences. S-Y, Ghim provided revisions to scientific content of manuscript. All authors participated this work and approved the final manuscript.
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Lee, SY., Son, JS., Hwang, YJ. et al. Metabacillus elymi sp. nov., isolated from the Rhizosphere of Elymus tsukushiensis, a plant native to the Dokdo Islands, Republic of Korea. Antonie van Leeuwenhoek 114, 1709–1719 (2021). https://doi.org/10.1007/s10482-021-01634-3
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DOI: https://doi.org/10.1007/s10482-021-01634-3