Abstract
Strains belonging to the Pseudomonas genus have been isolated worldwide from various biotic (humans, animals and plant tissues) and abiotic (food, soil, water and air) environments. Raw milk provides a favorable environment for the growth of a broad spectrum of microorganisms, including Pseudomonas. Here we present the description of Pseudomonas sp. UCMA 17988 isolated from raw milk, which was previously reported to produce new antimicrobial lipopeptides. MultiLocus Sequence Analysis of four housekeeping genes (16S rRNA, gyrB, rpoD and rpoB), whole genome sequence comparison (orthoANI value, original ANI value and dDDH value), microscopy, FAME analysis, and biochemical tests were performed. Digital DNA-DNA hybridization and average nucleotide identity values between strain UCMA 17988 and its closest relatives, P. helmanticensis CECT 8548T (46.9%, 92.07%) and P. baetica CECT 7720T (26.8%, 88.50%), rate well below the designed threshold for assigning prokaryotic strains to the same species. In conclusion, strain UCMA 17988 belongs to a novel species, for which the name Pseudomonas crudilactis sp. nov (type strain UCMA 17988T = DSM 109949T = LMG 31804T) is proposed.
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Funding
MS, ND and FJC express gratitude to the Organisation de Défense et de Gestion des AOP Laitières de Normandie for funding this research. CL is supported by a PhD fellowship from FWO Vlaanderen (1S64720N). LG and RDM acknowledge funding by EOS Grant 30650620 (RHIZOCLIP). The authors want to thanks Dr Richard Retoux and Mr Xavier Larose (CRISMAT UMR 6508, Normandie Univ, ENSICAEN, UNICAEN, CNRS, CRISMAT, 14000 Caen, France) for their support for microscopy observations.
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MS conceived, planned and carried out experiments and wrote the original draft. CL, DG, FJC, and LG carried out experiments. ND and RDM allowed funding acquisition. All authors provided critical feedback for the review of the manuscript.
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Schlusselhuber, M., Girard, L., Cousin, F.J. et al. Pseudomonas crudilactis sp. nov., isolated from raw milk in France. Antonie van Leeuwenhoek 114, 719–730 (2021). https://doi.org/10.1007/s10482-021-01552-4
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DOI: https://doi.org/10.1007/s10482-021-01552-4