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Haladaptatus pallidirubidus sp. nov., a halophilic archaeon isolated from saline soil samples in Yunnan and Xinjiang, China

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Abstract

Two extremely halophilic archaea, designated YIM 90917 and YIM 93656T, were isolated from saline soils in Yunnan province and Lup nur region in Xinjiang province, western China, respectively. Colonies of the two strains were observed to be pink-pigmented. The cells were found to be Gram-stain negative, coccoid and non-motile. The organisms were found to be aerobic and could grow in an NaCl range of 6–35 % (optimum 18 %), temperatures ranging from 25 to 50 °C (optimum 37–42 °C), pH range from 6.0–8.5 (optimum pH 7.0–7.5) and Mg2+ range from 0 to 1.5 M (optimum 0.5–1.0 M); Mg2+ was not necessary for growth. Cells were not observed to lyse in distilled water. Strains YIM 90917 and YIM 93656T showed the highest 16S rRNA gene sequence similarities to Haladaptatus cibarius JCM 15962T (97.6 and 97.9 %, respectively). In addition, the DNA–DNA hybridizations of strains YIM 90917 and YIM 93656T with type strains H. cibarius JCM 15962T, Haladaptatus litoreus JCM 15771T and Haladaptatus paucihalophilus JCM 13897T were 37.2 and 38.2 %, 36.6 and 39.0 % and 27.9 and 27.7 %, respectively. The DNA G+C contents of strains YIM 90917 and YIM 93656T were determined to be 56.0 and 57.4 mol%. The major polar lipids of the two strains were identified as phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, phosphatidylglycerol sulfate, sulfated mannosyl glucosyl diether and other four unidentified glycolipids. On the basis of physiological, chemotaxonomic data and phylogenetic analysis, the strains YIM 90917 and YIM 93656T can be classified as a novel species of the genus Haladaptatus, for which the name Haladaptatus pallidirubidus sp. nov. is proposed. The type strain is YIM 93656T (=JCM 17504T = CCTCC AB2010454T).

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Acknowledgements

We are grateful to Prof. Dr. George M. Garrity (Michigan State University, USA) for the Latin construction of the new taxon name, and Dr. Heng-Lin Cui (Jiangsu University, China) for his technical support and kind comments on this manuscript. This research was supported by National High-tech R & D Program of China (863 Program) (no. 2012AA021705). The authors extend their appreciation to the Deanship of Scientific Research at King Saud University for funding this work through the research group no. RGP-205. W-J Li was also supported by the ‘Hundred Talents Program’ of the Chinese Academy of Sciences and Guangdong Province Higher Vocational Colleges & Schools Pearl River Scholar Funded Scheme (2014).

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Authors and Affiliations

  1. Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education, Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, People’s Republic of China

    Bing-Bing Liu, Xiao Chu, Deene Manik Prabhu, Shu-Kun Tang & Wen-Jun Li

  2. Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin of Xinjiang Production & Construction Corps, Tarim University, Alar, 843300, People’s Republic of China

    Bing-Bing Liu & Li-Li Zhang

  3. State Key Laboratory of Biogeology and Environmental Geology, China University of Geosciences, Beijing, 100083, People’s Republic of China

    Wan-Yu Zhao

  4. Bioproducts Research Chair (BRC), College of Science, King Saud University, P.O. Box 2455, Riyadh, 11451, Kingdom of Saudi Arabia

    Wael N. Hozzein & Mohammed A. M. Wadaan

  5. Key Laboratory of Biogeography and Bioresource in Arid Land, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Ürűmqi, 830011, People’s Republic of China

    Wen-Jun Li

  6. State Key Laboratory of Biocontrol and Guangdong Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, People’s Republic of China

    Bing-Bing Liu & Wen-Jun Li

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  1. Bing-Bing Liu
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Correspondence to Wen-Jun Li.

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Bing–Bing Liu and Wan-Yu Zhao authors are contributed equally to this work.

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Liu, BB., Zhao, WY., Chu, X. et al. Haladaptatus pallidirubidus sp. nov., a halophilic archaeon isolated from saline soil samples in Yunnan and Xinjiang, China. Antonie van Leeuwenhoek 106, 901–910 (2014). https://doi.org/10.1007/s10482-014-0259-4

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