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Loktanella aestuariicola sp. nov., an alphaproteobacterium isolated from a tidal flat

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Abstract

A Gram-stain negative, aerobic, non-motile and coccoid, ovoid or rod-shaped bacterial strain, J-TF4T, which was isolated from a tidal flat in the South Sea of South Korea, was characterized taxonomically. Strain J-TF4T was found to grow optimally at 30 °C, at pH 7.0–8.0 and in presence of 2.0–3.0 % (w/v) NaCl. In the neighbour-joining phylogenetic tree based on 16S rRNA gene sequences, strain J-TF4T fell within the clade comprising the type strains of Loktanella species, clustering with the type strains of Loktanella cinnabarina, Loktanella hongkongensis, Loktanella soesokkakensis, Loktanella pyoseonensis and ‘Loktanella variabilis’ showing sequence similarity values of 97.2–98.4 %. The strain exhibited 16S rRNA gene sequence similarity values of 94.1–95.4 % to the type strains of the other Loktanella species. Strain J-TF4T was found to have Q-10 as the predominant ubiquinone and C18:1 ω7c as the major fatty acid. The major polar lipids of strain J-TF4T were identified as phosphatidylcholine, phosphatidylglycerol, diphosphatidylglycerol, an unidentified glycolipid and an unidentified aminolipid. The DNA G+C content of strain J-TF4T was determined to be 67.9 mol% and its mean DNA–DNA relatedness values with the type strains of five phylogenetically related Loktanella species were 17.7–23.3 %. Differential phenotypic properties, together with the phylogenetic and genetic data, demonstrate that strain J-TF4T is separated from other Loktanella species. On the basis of the data presented, strain J-TF4T is considered to represent a novel species of the genus Loktanella, for which the name Loktanella aestuariicola sp. nov. is proposed. The type strain is J-TF4T (=KCTC 42135T=NBRC 110408T).

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Acknowledgments

This work was supported by the Program for Collection, Management and Utilization of Biological Resources (grant NRF-2013M3A9A5075953) from the Ministry of Science, ICT & Future Planning (MSIP) of the Republic of Korea.

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Authors and Affiliations

  1. Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, South Korea

    Sooyeon Park, Yong-Taek Jung, Sung-Min Won, Ja-Min Park & Jung-Hoon Yoon

  2. University of Science and Technology (UST), 113 Gwahangno, Yuseong, Daejeon, South Korea

    Yong-Taek Jung

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  1. Sooyeon Park
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Correspondence to Jung-Hoon Yoon.

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Park, S., Jung, YT., Won, SM. et al. Loktanella aestuariicola sp. nov., an alphaproteobacterium isolated from a tidal flat. Antonie van Leeuwenhoek 106, 707–714 (2014). https://doi.org/10.1007/s10482-014-0240-2

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  • DOI: https://doi.org/10.1007/s10482-014-0240-2

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