Skip to main content
SpringerLink
Log in

Devosia epidermidihirudinis sp. nov. isolated from the surface of a medical leech

  • Short Communication
  • Published:
Antonie van Leeuwenhoek Aims and scope Submit manuscript

Abstract

A Gram-negative, rod-shaped organism, isolated from the surface of the medical leech Hirudo verbana was characterized phenotypically and genotypically. The calculated 16S rRNA gene sequence similarities to those of the most closely related species grouped strain E84T into the genus Devosia showing the highest similarities to Devosia limi (98.1 %), followed by Devosia psychrophila (97.9 %), Devosia neptuniae (97.3 %), and Devosia glacialis (97.5 %). Chemotaxonomic analyses showed that the major quinone was ubiquinone Q-10. The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol and three glycolipids. The major fatty acid profile consisted of C18:1 ω7c 11-methyl, C19:0 cyclo ω8c, and C16:0, C18:0 and C18:1 ω7c with C18:0 3OH as hydroxylated fatty acid. This profile is very similar to those of the patterns reported for the already described Devosia species. The results of DNA–DNA hybridization, physiological and biochemical tests allowed both genotypic and phenotypic differentiation of strain E84T from all other Devosia species suggesting a novel species for which the name Devosia epidermidihirudinis sp. nov. is proposed, with the type strain E84T (=CIP 110375T = LMG 26909T = CCM 8398T).

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

References

  • Bautista VV, Monsalud RG, Yokata A (2010) Devosia yakushimensis sp. nov., isolated from root nodules of Pueraria lobata (Willd.) Ohwi. Int J Syst Evol Microbiol 60:627–632

    Article  PubMed  CAS  Google Scholar 

  • Brosius J, Palmer ML, Kennedy PJ, Noller HF (1978) Complete nucleotide-sequence of a 16S ribosomal-RNA gene from Escherichia coli. PNAS 75:4801–4805

    Article  PubMed  CAS  Google Scholar 

  • Chun J, Lee J-H, Jung Y, Kim M, Kim S, Kim BK, Lim YW (2007) EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57:2259–2261

    Article  PubMed  CAS  Google Scholar 

  • Collins MD, Jones D (1980) Lipids in the classification and identification of coryneform bacteria containing peptidoglycans based on 2,4 diaminobutyric acid. J Appl Bacteriol 48:459–470

    Article  CAS  Google Scholar 

  • Collins MD, Pirouz T, Goodfellow M, Minnikin DE (1977) Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100:221–230

    PubMed  CAS  Google Scholar 

  • Felsenstein J (1985) Confidence limits of phylogenies: an approach using the bootstrap. Evolution 39:783–791

    Article  Google Scholar 

  • Felsenstein J (2005) PHYLIP (Phylogeny Inference Package) version 3.6. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle

    Google Scholar 

  • Foster JW (1944) Microbiological aspects of riboflavin. I. Introduction. II. Bacterial oxidation of riboflavin to lumichrome. J Bacteriol 47:27–41

    PubMed  CAS  Google Scholar 

  • Gerhardt P, Murray RG, Wood WA, Krieg NR (eds) (1994) Methods for general and molecular bacteriology. American Society for Microbiology, Washington, DC

    Google Scholar 

  • Glaeser SP, Galatis H, Martin K, Kämpfer P (2012) Luteolibacter cuticulihirudinis sp. nov., isolated from Hirudo medicinalis. Antonie Van Leeuwenhoek 102:319–324

    Article  PubMed  CAS  Google Scholar 

  • Glaeser SP, Galatis H, Martin K, Kämpfer P (2013) Castellaniella hirudinis sp. nov., isolated from the skin of Hirudo verbana. Int J Syst Evol Microbiol 63:521–525

    Google Scholar 

  • Groth I, Schumann P, Weiss N, Martin K, Rainey FA (1996) Agrococcus jenensis gen. nov., sp. nov., a new genus of actinomycetes with diaminobutyric acid in the cell wall. Int J Syst Evol Microbiol 46:234–239

    CAS  Google Scholar 

  • Jukes TH, Cantor CR (1969) Evolution of the protein molecules. In: Munro HN (ed) Mammalian protein metabolism. Academic Press, New York, pp 21–132

    Google Scholar 

  • Kämpfer P, Kroppenstedt RM (1996) Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol 42:989–1005

    Article  Google Scholar 

  • Kämpfer P, Steiof M, Dott W (1991) Microbiological characterization of a fuel-oil contaminated site including numerical identification of heterotrophic water and soil bacteria. Microb Ecol 21:227–251

    Article  Google Scholar 

  • Kämpfer P, Busse HJ, Longaric I, Rosselló-Móra R, Galatis H, Lodders N (2012) Pseudarcicella hirudinis gen. nov., sp. nov., isolated from the skin of the medical leech Hirudo medicinalis. Int J Syst Evol Microbiol 62:2247–2251

    Article  PubMed  Google Scholar 

  • Kumar M, Verma M, Lal R (2008) Devosia chinhatensis sp. nov., isolated from a hexachlorocyclohexane (HCH) dump site in India. Int J Syst Evol Microbiol 58:861–865

    Article  PubMed  CAS  Google Scholar 

  • Lane DJ (1991) 16S/23S rRNA sequencing nucleic acid techniques in bacterial systematics. In: Stackebrandt E, Goodfellow M (eds) Nucleic acid techniques in bacterial systematics. Wiley, Chichester, pp 115–175

    Google Scholar 

  • Lee SD (2007) Devosia subaequoris sp. nov., isolated from beach sediment. Int J Syst Evol Microbiol 57:2212–2215

    Article  PubMed  CAS  Google Scholar 

  • Ludwig W, Strunk O, Westram R, Richter L, Meier H et al (2004) ARB: a software environment for sequence data. Nucleic Acids Res 32:1363–1371

    Article  PubMed  CAS  Google Scholar 

  • Minnikin DE, Collins MD, Goodfellow M (1979) Fatty acid and polar lipid composition in the classification of Cellulomonas, Oerskovia and related taxa. J Appl Bacteriol 47:87–95

    Article  CAS  Google Scholar 

  • Nakagawa Y, Sakane T, Yokota A (1996) Transfer of “Pseudomonas riboflavina” (Foster 1944), a gram-negative, motile rod with long-chain 3-hydroxy fatty acids, to Devosia riboflavina gen. nov., sp. nov., nom. rev. Int J Bacteriol 46:16–22

    Article  CAS  Google Scholar 

  • Pruesse E, Quast C, Knittel K, Fuchs BM, Ludwig W, Peplies J, Glöckner FO (2007) SILVA: a comprehensive online resource for quality checked and aligned ribosomal RNA sequence data compatible with ARB. Nucleic Acid Res 35:7188–7196

    Article  PubMed  CAS  Google Scholar 

  • Rivas R, Willems A, Subba-Rao NS, Mateos PF, Dazzo FB, Kroppenstedt RM, Martínez-Molina E, Gillis M, Velázquez E (2003) Description of Devosia neptuniae sp. nov. that nodulates and fixes nitrogen in symbiosis with Neptunia natans, an aquatic legume from India. Syst Appl Microbiol 26:47–53

    Article  PubMed  CAS  Google Scholar 

  • Ryu SH, Chung BS, Le NT, Jang HH, Yun P-Y, Park W, Jeon CO (2008) Devosia geojensis sp. nov., isolated from diesel-contaminated soil in Korea. Int J Syst Evol Microbiol 58:633–636

    Article  PubMed  CAS  Google Scholar 

  • Stamatakis A (2006) RAxML-VI-HPC: maximum likelihood-based phylogenetic analyses with thousands of taxa and mixed models. Bioinformatics 22:2688–2690

    Article  PubMed  CAS  Google Scholar 

  • Vannini C, Rosati G, Verni F, Petroni G (2004) Identification of the bacterial endosymbionts of the marine ciliate Euplotes magnicirratus (Ciliophora, Hypotrichia) and proposal of ‘Candidatus Devosia euplotis’. Int J Syst Evol Microbiol 54:1151–1156

    Article  PubMed  CAS  Google Scholar 

  • Vanparys B, Heylen K, Lebbe L, De Vos P (2005) Devosia limi sp. nov., isolated from a nitrifying inoculum. Int J Syst Evol Microbiol 55:1997–2000

    Article  PubMed  CAS  Google Scholar 

  • Verma M, Kumar M, Dadhwal M, Kaur J, Lal R (2009) Devosia albogilva sp. nov. and Devosia crocina sp. nov., isolated from a hexychlorocyclohexane dump site. Int J Syst Evol Microbiol 59:795–799

    Article  PubMed  CAS  Google Scholar 

  • Yarza P, Richter M, Peplies J, Euzeby J, Amann R, Schleifer KH, Ludwig W, Glöckner FO, Rossello-Mora R (2008) The All-Species Living Tree project: a 16S rRNA-based phylogenetic tree of all sequenced type strains. Syst Appl Microbiol 31:241–250

    Article  PubMed  CAS  Google Scholar 

  • Yoo S-H, Weon HY, Kim BY, Hong S-B, Kwon S-W, Cho Y-H, Go S-J, Stackebrandt E (2006) Devosia soli sp. nov., isolated from greenhouse soil in Korea. Int J Syst Evol Microbiol 56:2689–2692

    Article  PubMed  CAS  Google Scholar 

  • Yoon J-H, Kang S-J, Park S, Oh T-K (2007) Devosia insulae sp. nov., isolated from soil, and emended description of the genus Devosia. Int J Syst Evol Microbiol 57:1310–1314

    Article  PubMed  CAS  Google Scholar 

  • Zhang D-C, Redzic M, Liu H-C, Zhou Y-G, Schinner F, Margesin R (2012) Devosia psychrophila sp. nov. and Devosia glacialis sp. nov., two novel bacteria from alpine glacier cryoconite. Int J Syst Evol Microbiol 62:710–715

    Article  PubMed  CAS  Google Scholar 

  • Ziemke F, Höfle MG, Lalucat J, Rosselló-Mora R (1998) Reclassification of Shewanella putrefaciens Owen’s genomic group II as Shewanella baltica sp. nov. Int J Syst Bacteriol 48:179–186

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

We thank Gundula Will and Maria Sowinsky for excellent technical assistance and Dr. J. Euzéby for his nomenclatural advice.

Author information

Authors and Affiliations

  1. Biebertaler Blutegelzucht, 35444, Biebertal, Germany

    Harald Galatis

  2. Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e.V., Hans-Knöll-Institut, 07745, Jena, Germany

    Karin Martin

  3. Institut für Angewandte Mikrobiologie, Justus-Liebig-Universität Giessen, Heinrich-Buff-Ring 26–32, 35392, Giessen, Germany

    Peter Kämpfer & Stefanie P. Glaeser

Authors
  1. Harald Galatis
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Karin Martin
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Peter Kämpfer
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Stefanie P. Glaeser
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Peter Kämpfer.

Additional information

The EMBL accession numbers for the 16S rRNA gene sequences of strain E84T is KC254735.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary material 1 (DOCX 1021 kb)

Rights and permissions

Reprints and permissions

About this article

Cite this article

Galatis, H., Martin, K., Kämpfer, P. et al. Devosia epidermidihirudinis sp. nov. isolated from the surface of a medical leech. Antonie van Leeuwenhoek 103, 1165–1171 (2013). https://doi.org/10.1007/s10482-013-9895-3

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10482-013-9895-3

Keywords

Search

Navigation