Abstract
New Delhi metallo-β-lactamase producing Pseudomonas aeruginosa isolates are of special interest since P. aeruginosa is a major cause of nosocomial infections, the treatment of which could now be jeopardized, especially in developing countries. Six additional NDM-1 positive P. aeruginosa clinical isolates belonging to two different genotypes were shown to be plasmid-free. PFGE-hybridization experiments revealed the chromosomal location of the bla NDM-1 gene. Restriction analysis and hybridization revealed that two copies of the bla NDM-1 gene are present in the genomes of all tested isolates, as in previously characterized P. aeruginosa MMA83. Moreover, it was shown that increasing imipenem concentration did not have the effect on copy number of the bla NDM-1 gene in the genome of P. aeruginosa MMA83.
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This work was supported by Grant 173019 from the Ministry of Education, Science and Technological Development of the Republic of Serbia.
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Jovčić, B., Lepšanović, Z., Begović, J. et al. Two copies of bla NDM-1 gene are present in NDM-1 producing Pseudomonas aeruginosa isolates from Serbia. Antonie van Leeuwenhoek 105, 613–618 (2014). https://doi.org/10.1007/s10482-013-0094-z
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DOI: https://doi.org/10.1007/s10482-013-0094-z