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Phenotypical and genetic characterization of Trichosporon sp. HP-2023. A yeast isolate from Las Yungas rainforest (Tucumán, Argentina) with dye-decolorizing ability

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Abstract

A basidiomycetous yeast isolated from Las Yungas rainforest (Tucumán, Argentina) and arbitrarily named HP-2023 was selected based on its outstanding textile dye decolorizing ability. Complete decolorization of Vilmafix® Red 7B-HE and Vilmafix® Blue RR-BB (200 mg/l) was achieved after 16 h of cultivation. Yeast characterization was accomplished by means of both traditional and molecular methods. Results concerning molecular fingerprinting and phenotypic characterization led to identify it as Trichosporon sp., closely related to the T. multisporumT. laibachii complex. The present work represents the first description of a Trichosporon yeast involved in reactive textile dye decolorization processes.

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References

  • Abadulla E, Tzanov T, Costa S, Robra KH, Cavaco-Paulo A, Gübitz GM (2000) Decolorization and detoxification of textile dyes with a laccase from Trametes hirsuta. Appl Environ Microbiol 66:3357–3362

    Article  PubMed  CAS  Google Scholar 

  • Akzu Z, Dönmez G (2003) A comparative study on the biosorption characteristics of some yeasts for Remazol Blue reactive dye. Chemosphere 50:1075–1083

    Article  Google Scholar 

  • Assas N, Marouani L, Hamdi M (2000) Scale down and optimization of olive mill wastewaters decolorization by Geotrichum candidum. Bioprocess Biosyst Eng 22:503–507

    CAS  Google Scholar 

  • Biswas SK, Yokoyama K, Nishimura K, Miyaji M (2001) Molecular phylogenetics of the genus Rhodotorula and related basidiomycetous yeasts inferred from the mitochondrial cytochrome b gene. Int J Syst Evol Microbiol 51:1191–1199

    PubMed  CAS  Google Scholar 

  • de Vrind-de Jong EW, Corstjens PLAM, Kempers ES, Westbroek P, deVrind JPM (1990) Oxidation of manganese and iron by Leptothrix discophora: use of N,N,N′,N′-tetramethyl-p-phenylenediamine as an indicator of metal oxidation. Appl Environ Microbiol 56:3458–3462

    Google Scholar 

  • Işik M, Sponza DT (2007) Fate and toxicity of azo dye metabolites under batch long-term anaerobic incubations. Enzyme Microb Technol 40:934–939

    Article  CAS  Google Scholar 

  • Fell JW, Boekhout T, Fonseca A, Scorzetti G, Statzell-Tallman A (2000) Biodiversity and systematics of basidiomycetous yeasts as determined by large-subunit rDNA D1/D2 domain sequence analysis. Int J Syst Evol Microbiol 50:1351–1371

    PubMed  CAS  Google Scholar 

  • Jarosz-Wilkołazka A, Kochmanska-Rdest J, Malarczyk E, Wardas W, Leonowicz A (2002) Fungi and their ability to decolourize azo and anthraquinonic dyes. Enzyme Microb Technol 30:566–572

    Article  Google Scholar 

  • Kalme S, Ghodake G, Govindwar S (2007) Red HE7B degradation using desulfonation by Pseudomonas desmolyticum NCIM 2112. Int Biodeterior Biodegradation doi:10.1016/j.ibiod.2007.05.06

  • Koroleff F (1976) Determination of ammonia. In: Grasshoft K (ed) Methods of seawater analysis. Verlag Chemie, pp 126–133

  • Kumar S, Tamura K, Nei M (2004) MEGA3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5:150–163

    Article  PubMed  CAS  Google Scholar 

  • Mesbah M, Premachandran U, Withman W (1989) Precise measurement of the G + C content of desoxyribonucleic acid by high performance liquid chromatography. Int J Syst Bacteriol 39:159–167

    Article  CAS  Google Scholar 

  • Middelhoven WJ (1991) Arxula adeninivorans, a yeast assimilating many nitrogenous and aromatic compounds. Antonie Van Leeuwenhoek 59:129–137

    Article  PubMed  CAS  Google Scholar 

  • Middelhoven WJ (1993) Catabolism of benzene compounds by ascomycetous and basidiomycetous yeasts and yeastlike fungi. A literature review and an experimental approach. Antonie van Leeuwenhoek 63:125–144

    Article  PubMed  CAS  Google Scholar 

  • Middelhoven WJ (1997) Assimilation of organic acids: the pH as determining factor. Yeast, a Newsletter for Persons Interested in Yeast 46:19–20

  • Middelhoven WJ, Scorzetti G, Fell JW (2000) Trichosporon veenhuisii sp. nov., an alkane-assimilating anamorphic basidiomycetous yeast. Int J Syst Evol Microbiol 50:381–387

    PubMed  CAS  Google Scholar 

  • Middelhoven WJ, Scorzetti G, Fell JW (2001) Trichosporon porosum, comb.nov., an anamorphic basidiomycetous yeast inhabiting soil, related to the loubieri/laibachii group of species that assimilate hemicelluloses and phenolic compounds. FEMS Yeast Res 1:15–22

    PubMed  CAS  Google Scholar 

  • Middelhoven WJ, Scorzetti G, Fell JW (2004) Systematics of the anamorphic basidiomycetous yeast genus Trichosporon Behrend with the description of five novel species: Trichosporon vadense, T. smithiae, T. dehoogii, T. scarabaeorum and T. gamsii. Int J Syst Evol Microbiol 54:975–986

    Article  PubMed  CAS  Google Scholar 

  • Miller GL, Blum R, Glennon WE (1959) Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal Chem 31:426–428

    Article  CAS  Google Scholar 

  • Nigam P, Armour G, Banat I M, Singh D, Marchant R (2000) Physical removal of textile dyes from effluents and solid-state fermentation of dye-adsorbed agricultural residues. Bioresour Technol 72:219–226

    Article  CAS  Google Scholar 

  • Novotny C, Rawal B, Bhatt M, Patel M, Sasek V, Molitoris HP (2001) Capacity of Irpex lacteus and Pleurotus ostreatus for decolorization of chemically different dyes. J Biotechnol 89:113–122

    Article  PubMed  CAS  Google Scholar 

  • Oren A, Gurevich P, Henis Y (1991) Reduction of nitrosubstituted aromatic compounds by the halophilic anaerobic eubacteria Haloanaerobium praevalens and Sporohalobacter marismortui. Appl Environ Microbiol 57:3367–3370

    PubMed  CAS  Google Scholar 

  • Pajot HF, de Figueroa LIC, Fariña JI (2007) Dye-decolorizing activity in isolated yeasts from the ecoregion of Las Yungas (Tucumán, Argentina). Enzyme Microb Technol 40:1503–1511

    Article  CAS  Google Scholar 

  • Ramalho AP, Scholze H, Cardozo MH, Ramalho MT, Oliveira-Campos AM (2002) Improved conditions for the aerobic reductive decolourisation of azo dyes by Candida zeylanoides. Enzyme Microb Technol 31:848–854

    Article  CAS  Google Scholar 

  • Scorzetti G, Fell JW, Fonseca A, Statzell-Tallman A (2002) Systematics of basidiomycetous yeasts: a comparison of large subunit D1/D2 and internal transcribed spacer rDNA regions. FEMS Yeast Res 2:495–517

    PubMed  CAS  Google Scholar 

  • Stolz A (2001) Basic and applied aspects in the microbial degradation of azo dyes. Appl Microbiol Biotechnol 56:69–80

    Article  PubMed  CAS  Google Scholar 

  • Tien M, Kirk TK (1984) Lignin-degrading enzyme from Phanerochaete chrysosporium: purification, characterization and catalytic properties of a unique H2O2 requiring oxygenase. Proc Natl Acad Sci USA 81:2280–2284

    Article  PubMed  CAS  Google Scholar 

  • Thompson JD, Higgins DG, Gibson TJ (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680

    Article  PubMed  CAS  Google Scholar 

  • White TJ, Bruns T, Lee S, Taylor J (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TT (eds) PCR protocols. a guide to methods and applications. Academic Press, San Diego, CA

    Google Scholar 

  • Yarrow D (1998) Methods for the isolation, maintenance and identification of yeasts. In: Kurtzman CP, Fell JW (eds) The yeasts, a taxonomic study, 4th edn. Elsevier Science B.V., Amsterdam, pp 77–100

    Chapter  Google Scholar 

  • Yu Z, Wen X (2005) Screening and identification of yeasts for decolorizing synthetic dyes in industrial wastewater. Int Biodeterior Biodegradation 56:109–14

    Article  CAS  Google Scholar 

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Acknowledgements

Thanks are due to Dr. W.J. Middelhoven for advice concerning Trichosporon identification and to Dr. O.D. Delgado for supervision and helpful discussions concerning yeast molecular characterization. This work was supported by Agencia Nacional de Promoción Científica y Tecnológica-FONCYT (PICT2003-14496), Consejo Nacional de Investigaciones Científicas y Técnicas, CONICET (PIP 6202), and Consejo de Investigaciones de la Universidad Nacional de Tucumán, CIUNT (D-311).

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Correspondence to Hipólito F. Pajot.

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Pajot, H.F., Figueroa, L.I.C., Spencer, J.F.T. et al. Phenotypical and genetic characterization of Trichosporon sp. HP-2023. A yeast isolate from Las Yungas rainforest (Tucumán, Argentina) with dye-decolorizing ability. Antonie van Leeuwenhoek 94, 233–244 (2008). https://doi.org/10.1007/s10482-008-9236-0

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