Skip to main content
SpringerLink
Log in

Genome-wide expression profile of the mnn2Δ mutant of Saccharomyces cerevisiae

  • Published:
Antonie van Leeuwenhoek Aims and scope Submit manuscript

Abstract

The MNN2 gene of S. cerevisiae encodes an α (1,2) mannosyl transferase required for branching the outer chain of N-linked oligosaccharides (Rayner J.C. and Munro S. 1998. J. Biol. Chem. 273: 26836–26843) and it also seems to have some effect on the transfer of mannosyl phosphate groups to the inner core (Olivero I. et al. 2000. FEBS Lett. 475: 111–116). In order to reveal possible interactions of MNN2 expression with other cellular pathways, we analyzed the transcriptome of the deletion mutant S. cerevisiae  mnn2Δ using cDNA microarrays. We found 151 genes that showed an altered expression level of ≥2-fold, 58 of them up-regulated and 93 down-regulated. Quite a high proportion of these genes (29%) encode unclassified proteins. In contrast to other defects affecting the integrity of the cell wall, deletion of MNN2 does not stimulate the expression of any of the genes included in the previously defined ‘cell wall compensatory cluster’ (Lagorce et al. 2003. J. Biol. Chem. 278: 20345–20357). We also found that 15% of the selected genes are related to central metabolic pathways. In addition, the mnn2Δ strain seems to have a certain level of stimulation of DNA processing reactions while some genes involved in intracellular transport pathways are under-regulated.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

CWI:

cell wall integrity pathway

ldb:

low dye binding

mnn:

mannan defective

References

  • Ballou C.E. (1990). Isolation, characterization and properties of Saccharomyces cerevisiae mnn mutants with non-conditional protein glycosylation defects. Methods Enzymol. 185: 440–470

    Article  PubMed  CAS  Google Scholar 

  • Boorsma A., de Nobel H., ter Riet B., Bargmann B., Brul S., Hellingwerf K.J. and Klis F.M. (2004). Characterization of the transcriptional response to cell wall stress in Saccharomyces cerevisiae. Yeast 21: 413–427

    Article  PubMed  CAS  Google Scholar 

  • Brazma A., Hingamp P., Quackenbush J., Sherlock G., Spellman P., Stoeckert C., Aach J., Ansorge W., Ball C.A., Causton H.C., Gaasterland T., Glenisson P., Holstege F.C., Kim I.F., Markowitz V., Matese J.C., Parkinson H., Robinson A., Sarkans U., Schulze-Kremer S., Stewart J., Taylor R., Vilo J. and Vingron M. (2001). Minimum information about a microarray experiment (MIAME)-toward standards for microarray data. Nat. Genet. 29: 365–371

    Article  PubMed  CAS  Google Scholar 

  • Burda P. And Aebi M. (1999). The dolichol pathway of N-linked glycosylation. Biochim. Biophys. Acta. 1426: 239–258

    PubMed  CAS  Google Scholar 

  • Corbacho I., Olivero I. and Hernández L.M. (2004). Identification of low dye binding (ldb) mutants of Saccharomyces cerevisiae. FEMS Yeast Res. 4: 437–444

    Article  PubMed  CAS  Google Scholar 

  • Corbacho I., Olivero I. and Hernández L.M. (2005). A genome-wide screen for Saccharomyces cerevisiae nonessential genes involved in mannosyl phosphate transfer to mannoprotein-linked oligosaccharides. Fungal Genet. Biol. 42: 773–790

    Article  PubMed  CAS  Google Scholar 

  • Dean N. (1999). Asparagine-linked glycosylation in the yeast Golgi. Biochim. Biophys. Acta 1426: 309–322

    PubMed  CAS  Google Scholar 

  • De Groot P.W.J., Ram A.F. and Klis F.M. (2005). Features and functions of covalently linked proteins in fungal cell walls. Fungal. Genet. Biol. 42: 657–675

    Article  PubMed  CAS  Google Scholar 

  • Erasmus D.J., van der Merwe G.K. and van Vuuren H.J. (2003). Genome-wide expression analyses: Metabolic adaptation of Saccharomyces cerevisiae to high sugar stress. FEMS Yeast Res. 3: 375–399

    Article  PubMed  CAS  Google Scholar 

  • Hauser N.C., Vingron M., Scheideler M., Krems B., Hellmuth K., Entian K.D. and Hoheisel J.D. (1998). Transcriptional profiling on all open reading frames of Saccharomyces cerevisiae. Yeast 14: 1209–1221

    Article  PubMed  CAS  Google Scholar 

  • Hernández L.M., Ballou L., Alvarado E., Tsai P.K. and Ballou C.E. (1989). Structure of the phosphorylated N-linked oligosaccharides from the mnn9 and mnn10 mutants of Saccharomyces cerevisiae. J. Biol. Chem. 264: 13648–13659

    PubMed  Google Scholar 

  • Kleinschmidt M., Grundmann O., Bluthgen N., Mosch H.U. and Braus G.H. (2005). Transcriptional profiling of Saccharomyces cerevisiae cells under adhesion-inducing conditions. Mol. Genet. Genomics 273: 382–393

    Article  PubMed  CAS  Google Scholar 

  • Klis F.M., De Groot P., Brul S. and Hellingwerf K. 2004. Molecular organization and biogenesis of the cell wall. In: Dickinson J.R., Schweizer M. (eds), The Metabolism and Molecular Physiology of Saccharomyces Cerevisiae. 2nd ed. CRC Press, Boca Raton, FL, pp. 117–139.

  • Lagorce A., Hauser N.C., Labourdette D., Rodriguez C., Martin-Yken H., Arroyo J., Hoheisel J.D. and Francois J. (2003). Genome-wide analysis of the response to cell wall mutations in the yeast Saccharomyces cerevisiae. J. Biol. Chem. 278: 20345–20357

    Article  PubMed  CAS  Google Scholar 

  • Levin D.E. (2005). Cell wall integrity signalling in Saccharomyces cerevisiae. Microbiol. Mol. Biol. Rev. 69: 262–291

    Article  PubMed  CAS  Google Scholar 

  • Mañas P., Olivero I., Avalos M. and Hernández L.M. (1997). Isolation of new nonconditional Saccharomyces cerevisiae mutants defective in asparagine-linked glycosylation. Glycobiology 7: 487–497

    PubMed  Google Scholar 

  • Mañas P., Olivero I. and Hernández L.M. (1998). Proteolytic processing of a secreted glycoprotein and O-glycosylation of mannoproteins are affected in the N-glycosylation mutant Saccharomyces cerevisiae ldb1. Biochim. Biophys. Acta 1380: 320–328

    PubMed  Google Scholar 

  • Nakamura T., Ohmoto T., Hirata D., Tsuchiya E., Miyakawa T. (1997). Yeast Crv4/Ttp1, a predicted type II membrane protein, is involved in an event important for growth, functionally overlapping with the event regulated by calcineurin- and Mpk1-mediated pathways. Mol. Gen. Genet. 256: 481–487

    Article  PubMed  CAS  Google Scholar 

  • Olivero I., Mañas P. and Hernández L.M. (2000). The mnn2 mutant of accharomyces cerevisiae is affected in phosphorylation of N-linked oligosaccharides. FEBS Lett. 475: 111–116

    Article  PubMed  CAS  Google Scholar 

  • Olivero I., Corbacho I. and Hernández L.M. (2003). The ldb1 mutant of Saccharomyces cerevisiae is defective in Pmr1p, the yeast secretory pathway/Golgi Ca2+/Mn2+-ATPase. FEMS Microbiol. Lett. 219: 137–142

    Article  PubMed  CAS  Google Scholar 

  • Rayner J.C. and Munro S. (1998). Identification of the MNN2 and MNN5 mannosyl transferases required for forming and extending the mannose branches of the outer chain mannans of Saccharomyces cerevisiae. J. Biol. Chem. 273: 26836–26843

    Article  PubMed  CAS  Google Scholar 

  • Tong A.H., Lesage G., Bader G.D., Ding H., Xu H., Xin X., Young J., Berriz G.F., Brost R.L., Chang M., Chen Y., Cheng X., Chua G., Friesen H., Goldberg D.S., Haynes J., Humphries C., He G., Hussein S., Ke L., Krogan N., Li Z., Levinson J.N., Lu H., Menard P., Munyana C., Parsons A.B., Ryan O., Tonikian R., Roberts T., Sdicu A.M., Shapiro J., Sheikh B., Suter B., Wong S.L., Zhang L.V., Zhu H., Burd C.G., Munro S., Sander C., Rine J., Greenblatt J., Peter M., Bretscher A., Bell G., Roth F.P., Brown G.W., Andrews B., Bussey H., Boone C. (2004) Global mapping of the yeast genetic interaction network. Science 303: 808–813

    Article  PubMed  CAS  ADS  Google Scholar 

  • Zakrzewska A., Boorsma A., Brul S., Hellingwerf K.J. and Klis F.M. (2005). Transcriptional response of Saccharomyces cerevisiae to the plasma membrane-perturbing compound chitosan. Eukaryot. Cell 4:703–715

    Article  PubMed  CAS  Google Scholar 

Web pages

  • EUROSCARF (European Saccharomyces cerevisiae archive for functional analysis): http://www.uni-frankfurt.de/fb15/mikro/euroscarf/index.html

  • MIPS (Munich Information center for protein sequences): http://mips.gsf.de/

  • SGD (Saccharomyces cerevisiae genome database): http://www.yeastgenome.org/

Download references

Acknowledgements

Part of this work was performed by I.C. as a visiting student at the Marie Curie Training Site “Göteborg Yeast Centre”, Göteborg, Sweden; EC contract QLK-CT2000-60036. The entire project is supported by grant 2PR03A080 from the Junta de Extremadura. I.C. is the recipient of a pre-doctoral grant from the Junta de Extremadura.

Author information

Authors and Affiliations

  1. Department of Microbiology, University of Extremadura, 06071, Badajoz, Spain

    Isaac Corbacho, Isabel Olivero &  Luis M. Hernández

  2. Department of Cell and Molecular Biology, Göteborg University, S-405 30, Göteborg, Sweden

    Stefan Hohmann & Per Sunnerhagen

Authors
  1. Isaac Corbacho
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Isabel Olivero
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Stefan Hohmann
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Per Sunnerhagen
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Luis M. Hernández
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Luis M. Hernández.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Corbacho, I., Olivero, I., Hohmann, S. et al. Genome-wide expression profile of the mnn2Δ mutant of Saccharomyces cerevisiae . Antonie Van Leeuwenhoek 89, 485–494 (2006). https://doi.org/10.1007/s10482-005-9047-5

Download citation

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10482-005-9047-5

Keywords

Search

Navigation