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High-frequency callus organogenesis, large-scale cultivation and assessment of clonal fidelity of regenerated plants of Curcuma caesia Roxb., an important source of camphor

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Abstract

An efficient in vitro propagation protocol has been standardized for Curcuma caesia Roxb., an important source of camphor, using bud- and leaf-derived callus. The optimum response of 84 and 71 % callus induction was obtained when bud and leaf segment explants were cultured on Murashige and Skoog (MS) medium supplemented with 6.7 µM 2, 4-dichlorophenoxyacetic acid (2, 4-D) and 2.7 µM naphthalene acetic acid (NAA). The white, friable, organogenic calli were subcultured on MS medium supplemented with 6.8 µM thidiazuron (TDZ) and 1.6 µM NAA for shoot induction. On this medium, 90 % of the bud-derived calli responded with an average number of 16.2 shoots per culture. Comparatively, bud-derived calli demonstrated a better regeneration response than leaf calli. In vitro rooting of shoots was also obtained on the regeneration medium. The rooted shoots were successfully hardened and transferred to field conditions. Random amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) analysis revealed no evidence of genetic variation in all 22 plants established from the callus with the parental plant, suggesting this protocol could be used for large-scale true-to-type propagation and multiplication of elite clones of C. caesia.

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Abbreviations

BA:

N6-benzylaminopurine

2, 4-D:

2, 4-Dichlorophenoxyacetic acid

FW:

Fresh weight

Kn:

Kinetin

MS:

Murashige and Skoog medium

NAA:

Naphthalene acetic acid

RAPD:

Random amplified polymorphic DNA

TDZ:

Thidiazuron

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Acknowledgments

We thank the Principal, St. Thomas College, Palai, for providing the necessary facilities.

Conflict of interest

The authors declare that they have no conflicts of interest concerning this article.

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  1. T. Dennis Thomas

    Present address: Field Science Centre for Northern Biosphere, Hokkaido University, Kita 11, Nishi 10, Kitaku, Sapporo, 060-0811, Japan

Authors and Affiliations

  1. Research and Development Center, Bharathiar University, Maruthamalai Road, Coimbatore, 641046, Tamil Nadu, India

    Sweetymol Jose

  2. Postgraduate and Research Department of Botany, St. Thomas College, Palai, Arunapuram (P.O), Kottayam, 686 574, Kerala, India

    Sweetymol Jose & T. Dennis Thomas

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Jose, S., Thomas, T.D. High-frequency callus organogenesis, large-scale cultivation and assessment of clonal fidelity of regenerated plants of Curcuma caesia Roxb., an important source of camphor. Agroforest Syst 89, 779–788 (2015). https://doi.org/10.1007/s10457-015-9811-0

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