Abstract
The applications of silica-based nanomaterials in dental fields as multifunctional scaffolds and carriers have been widely documented in recent years. However, toxicity of this type of nanoparticles in dental cells has not been elucidated in detail. The aim of present study was to investigate the effects of naked and PEGylated silica nanoparticles on cementoblasts. Methods including MTT assay, apoptosis, LDH, as well as ROS analysis were introduced in our study. Moreover, ALP analysis and alizarin red staining were additionally performed to indicate the influence of SiO2 and PEG-SiO2 on cementoblast differentiation. Results obtained from our designs and experiments demonstrated that naked silica nanoparticles could induce more cell toxicity than PEG-SiO2 nanoparticles, indicating PEGylation could efficiently reduce in vitro toxicity from several sections including ROS, LDH, and other important routes. Based on above results, we concluded that it was so necessary to process PEGylation while silica-based materials were applied in biomedicine and related fields including dental area.
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Abbreviations
- PEG:
-
Polyethylene glycol
- ROS:
-
Reactive oxygen species
- ALP:
-
Alkaline phosphatase
- LDH:
-
Lactate dehydrogenase
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Acknowledgments
Cementoblast cell line (OCCM-30) was obtained as a gift from Prof. Somerman in University of Washington. Otherwise, we also thanked for their assistance in cell experiments. This work was supported by National Natural Science Foundation of China, NSFC 81170999; Foundation from Department of Health of Jilin Province, China, 2011Z071; and Specialized Research Fund for Doctoral Program of Higher Education, China, SRFDP 20110061110072.
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The authors have declared that no competing interest exists.
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Associate Editor K. A. Athanasiou oversaw the review of this article.
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Bao, X., Wei, X., Wang, Y. et al. Effect of Silica-Based Nanomaterials and Their Derivate with PEGylation on Cementoblasts. Ann Biomed Eng 42, 1781–1789 (2014). https://doi.org/10.1007/s10439-014-1012-x
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DOI: https://doi.org/10.1007/s10439-014-1012-x