Abstract
This work is focused on developing a new chromatographic method with UV detection for the separation and determination of thirty-two carboxylic aliphatic and/or aromatic acids in a single run. Ion-exclusion chromatography with a silica based, modified with C18, analytical column Alltech Prevail™ organic acid 5 µm (150 mm × 4.6 mm I.D) was used for the solving of this problem. The developed method was based on ion-exclusion and/or hydrophobic interaction chromatographic separation mechanism. The effect of the concentrations of phosphate buffer and its pH as well as the column temperatures on the retention of the test acids has been investigated. Gradient elution of the mobile phase composed of aqueous phosphate buffer and methanol was used to achieve a required separation of carboxylic acids within 45 min. All measurements were done at 220 nm. Column temperature was set at 25 ± 0.1 °C. The LOD values for organic acids range from 0.002–2.224 mg L−1. The repeatability of the procedure developed is characterized by the RSD, which varied between 0.52 and 2.85 % for the peak area. The proposed method was successfully applied for the determination of aliphatic and aromatic acids in dry white wine and human urine samples.
Similar content being viewed by others
References
Van Hess PAW, Vinogradoff SI (2003) Soil Biol Biochem 35:1015–1026
Van Hess PAW (2002) Soil Biol Biochem 34:1261–1272
Peres RG, Moraes EP (2009) Food Control 20:548–552
Waksmundzka-Hajnos M (1998) J Chromatogr B 717:93–118
Goodman LS, Gilman A (1996) In: Hardman JG, Limbird LE (eds) The pharmacological basis of therapeutics, 9th edn. McGraw-Hill, New York
Castineira A (2002) J Food Compos Anal 15:319–331
Mato I, Suarez-Luque S, Huidobro JF (2007) Food Chem 102:104–112
Kenney BF (1991) J Chromatogr 546:423–430
De Villiers A, Lynen F, Crouch A, Sandra P (2003) Eur Food Res Technol 217:535–540
Bandyopadhyay C (1990) J Agr Food Chem 38:1696–1699
Luczak S (1989) Acta Pol Pharm 46:381–385
Montano A (1995) Analyst 120:2483–2487
Jham GN (2002) Phytochem Anal 13:99–104
Gey M (1988) Acta Biotechnol 8:197
Klinke HB, Ahring BK, Schmidt AS (2002) Bioresour Technol 82:15
Tusseau D, Benoit C (1987) J Chromatog A 395:323–333
Saccani G (1995) J Chromatog A 706:395–403
Guillén DA (1996) J Chromatog A 730:39–46
Pietta PG (1997) J Chromatog B 693:249–255
Amin Ruhul M (1995) J Chromatog B 663:201–207
Li LS (2004) Talanta 62:643–648
Guillén DA (1996) J Chromatog A 730:39–46
Wheaton RM, Bauman WC (1953) Ann Ny Acad Sci 57:159–176
Ohta K, Tanak K, Haddad PR (1996) J Chromatog A 739:359–365
Ohta K, Tanak K (1999) J Chromatog A 850:177–185
Glod BK, Stafiej J (1993) J Chromatog A 654:197–205
Ng KL (1999) J Chromatog A 850:17–27
Albarrán G, Collins CH (1987) J Chromatog A 395:623–629
Tanaka K (1995) J Chromatog A 706:385–393
Tanaka K, Ohta K, Fritz JS (1997) J Chromatog A 70:211–218
Ohta K, Ohashi M (2003) Anal Chim Acta 481:15–21
Chen ZL, Adams MA (1999) Anal Chim Acta 386:249–256
Ng KL (1999) J Chromatog A 850:17–27
Ohta K (2003) J Chromatog A 997:117–125
Crespo P, Bordonaba JG, Terry LA, Carlen C (2010) Food Chem 122:16–24
Li J, Jiang M, Chen K (2010) Process Biochem 45:980–985
Li WC, Ye ZH, Wong MH (2007) J Exp Bot 58:4173–4182
Lindsay S (1992) High performance liquid chromatography. Wiley, New York
Rinaldo P, Hahn S, Matern D (2006) In: Burtis CA, Ashwood ER, Bruns DE (eds) Tietz textbook of clinical chemistry and molecular diagnostics, 4th edn. Elsevier Saunders, St. Louis
Farkaš J (1983) Biotechnológia vína. Alfa, Bratislava
Acknowledgments
This work was generously supported by the grant of project MŠ SR VEGA 1/0852/13, MŠ SR VEGA 1/1349/12, the grant of project APVV-0583/11 and grant UK/232/2014.
Author information
Authors and Affiliations
Corresponding author
Additional information
Published in the special paper collection 19th International Symposium on Separation Sciences with guest editors Tomislav Bolanča and Bogusław Buszewski.
Rights and permissions
About this article
Cite this article
Halko, R., Hukelová, I. Single-Run Separation and Determination of Aliphatic and Aromatic Carboxylic Acids in Wine and Human Urine Samples by Ion-Exclusion Chromatography. Chromatographia 77, 1037–1046 (2014). https://doi.org/10.1007/s10337-014-2706-9
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10337-014-2706-9