Abstract
A simple and sensitive liquid chromatography with ultraviolet detection (LC–UV) method was developed for the determination of three impurities with a content over 0.1% (w/w) in technical triadimefon. A Gemini C18 column (5 μm, 250 mm × 4.6 mm i.d.) was used for the chromatographic separations. The samples were separated by gradient elution with water (solvent A) and methanol (solvent B) using the following conditions: 70% A isocratic for 12 min, linear to 0% A within 8 min, and isocratic for 10 min at 0% A with a flow rate of 1.0 mL min−1. Chromatograms were recorded at an absorption wavelength of 280 nm. The chromatographic resolutions between triadimefon and its potential impurities A, B, and C were greater than 3. The developed LC method was validated with respect to linearity, accuracy, precision, and robustness. This method was successfully applied to analyze the impurities in commercial technical triadimefon. In addition, the structures of the three impurities were identified to be (A) 4-chlorophenol, (B) 1-(2,4-dichlorophenoxy)-3,3-dimethyl-1-(1H-1,2,4-triazol-1-yl)-2-butanone, and (C) 1,1-bis(4-chlorophenoxy)-3,3-dimethyl-2-butanone.
References
Walker QD, Lewis MH, Crofton KM, Mailman RB (1990) Toxicol Appl Pharmacol 102:474–485
Slahck SC, Bennett OO, Folsom BD, Gentry GM, Hodgins WE, Kruger H, Mummah LA, Plunkett RW, Tengler H, Tomkins DF (1985) J Assoc Off Anal Chem 68:586–589
Partha PC, Dureja P (1994) Toxicol Environ Chem 46:213–216
Wang X, Lin JB, Song F, Zhang YZ, Wang M (2004) Chin J Pestic 43:212–213
Lee WO, Wong SK (1995) Analyst 120:2475–2478
Sannino A, Bandini M, Bolzoni L (1999) J AOAC Int 82:1229–1238
Tang F, Yue Y, Hua R, Cao H (2006) J AOAC Int 89:498–502
Cairns T, Siegmund EG, Chiu KS, Nelson R (1989) Biomed Environ Mass Spectrom 18:110–115
González-Rodríguez RM, Rial-Otero R, Cancho-Grande B, Simal-Gándara J (2008) J Chromatogr A 1196–1197:100–109
Stamatis N, Hela D, Konstantinou I (2010) J Hazard Mater 175:829–835
Millán S, Sampedro MC, Unceta N, Goicolea MA, Rodríguez E, Barrio RJ (2003) J Chromatogr A 995:135–142
Tang T, Qian K, Shi T, Wang F, Li J, Cao Y (2010) Anal Chim Acta 680:26–31
Geerdink RB, Kooistra-Sijpersma A, Tiesnitsch J, Kienhuis PG, Brinkman UAT (1999) J Chromatogr A 863:147–155
Juan-García A, Mañes J, Font G, Picó Y (2004) J Chromatogr A 1050:119–127
Nozal MJ, Bernal JL, Jiménez JJ, Martín MT, Bernal J (2005) J Chromatogr A 1076:90–96
Schermerhorn PG, Golden PE, Krynitsky AJ, Leimkuehler WM (2005) J AOAC Int 88:1491–1502
Trösken ER, Bittner N, Völkel W (2005) J Chromatogr A 1083:113–119
Gilbert-López B, García-Reyes JF, Ortega-Barrales P, Molina-Díaz A, Fernández-Alba AR (2007) Rapid Commun Mass Spectrom 21:2059–2071
Wu YS, Lee HK, Li SF (2000) Electrophoresis 21:1611–1619
Wu YS, Lee HK, Li SF (2001) J Chromatogr A 912:171–179
Wang P, Jiang S, Liu D, Wang P, Zhou Z (2005) J Biochem Biophys Methods 62:219–230
Zhou Y, Li L, Lin K, Zhu X, Liu W (2009) Chirality 21:421–427
Author information
Authors and Affiliations
Corresponding authors
Rights and permissions
About this article
Cite this article
Su, Mx., You, Lj., Di, B. et al. A Validated LC Method for Determination of Trace Impurities in Technical Triadimefon. Chromatographia 74, 737–741 (2011). https://doi.org/10.1007/s10337-011-2131-2
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10337-011-2131-2