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LC–MS Analysis of Sertraline and Its Active Metabolite in Human Serum Using a Silica Column with a Non-Aqueous Polar Mobile Phase

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Abstract

A sensitive liquid chromatography–mass spectrometry method for the simultaneous determination of sertraline (SER) and its major metabolite norsertraline (NOR) from serum was developed and validated in the context of a pharmacokinetic study in pregnant women. The separations were achieved on a silica column with a non-aqueous polar mobile phase consisting of acetonitrile, methanol and ammonium acetate at a flow rate of 0.5 mL min−1. The concentrations were measured using a single quadruple mass spectroscopic detector supplied with atmospheric pressure ionization electrospray. Sample preparation consisted of a simple liquid–liquid procedure. The detector was set in selective ion mode for each compound of interest, 306 m/z for SER and 275 m/z for NOR. Calibration curves were generated by least square linear regression for concentration of 5–160 ng mL−1 for SER and from 10 to 320 ng mL−1 for NOR. The curves for both compounds of interest were linear, with correlation coefficients r 2 ≥ 0.999.

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Acknowledgments

The authors would like to thank Pfizer Pharmaceutical for its generosity in supplying primary standards for both SER and its metabolite NOR. We are also grateful to the staff at the University of Arizona GCRC, Tucson, AZ for drawing the timed samples and processing all the blood. In addition, we would like to thank Drs. Anne-Francoise Aubry and David K. Lloyd for their invaluable help in editing this manuscript. Foremost, we are indebted to both the FDA Office of Women’s Health and NIMH for providing the funding for this project. Finally, we thank Dr. David Bishop for proofreading the manuscript and preparing the final version of the figures.

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Authors and Affiliations

  1. Department of Pharmacology and Toxicology, University of Arizona College of Pharmacy, Tucson, AZ, USA

    Karen M. Fried

  2. Department of Pharmacy Practice and Science, University of Arizona College of Pharmacy, Tucson, AZ, USA

    Paul E. Nolan Jr.

  3. The Critical Path Institute, Tucson, AZ, USA

    Marietta Anthony & Raymond L. Woosley

  4. Department of Psychiatry, Massachusetts General Hospital, Boston, MA, USA

    Marlene P. Freeman

Authors
  1. Karen M. Fried
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  2. Paul E. Nolan Jr.
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  3. Marietta Anthony
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  4. Raymond L. Woosley
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  5. Marlene P. Freeman
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Corresponding author

Correspondence to Marlene P. Freeman.

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Fried, K.M., Nolan, P.E., Anthony, M. et al. LC–MS Analysis of Sertraline and Its Active Metabolite in Human Serum Using a Silica Column with a Non-Aqueous Polar Mobile Phase. Chromatographia 73, 749–754 (2011). https://doi.org/10.1007/s10337-011-1919-4

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  • DOI: https://doi.org/10.1007/s10337-011-1919-4

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