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Soft rot disease alters soil characteristics and root-associated, culturable microbial community of Amorphophallus konjac

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Abstract

To elucidate how soft rot disease affects soil characteristics and root-associated, culturable microorganisms in Amorphophallus konjac stands, the responses of soil around roots of A. konjac with soft rot disease were investigated in stands with and without soft rot. Changes in the root-associated culturable microbial community and diversity were investigated by dilution plating. Soil characteristics were compared between stands using standard techniques. A. konjac with soft rot had higher concentrations of available soil P and K, NH4–N, organic matter and water content and lower pH compared with plants without. The community composition of root-associated culturable microorganisms differed between stands with and without soft rot. The microbial community associated with soft rot in A. konjac was characterized by four types of abundant microorganisms (Fusarium solani, Fusarium oxysporum, Pseudomonas chlororaphis subsp. aureofaciens and Stenotrophomonas pavanii) and three types of less-abundant microorganisms (Rhizobium radiobacter, Bacillus thuringiensis and Streptomyces cellulosae), and a small number of Bacillus and Streptomyces species in the rhizosphere and rhizoplane soils. Particular microbial combinations were diametrically opposed between plants with and without soft rot. The richness and diversity of root-associated culturable microorganisms were higher in the stand without soft rot than in the stand with soft rot. A. konjac soft rot led to obvious differences in the diversity and community composition of root-associated culturable microorganisms and in soil characteristics.

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Acknowledgements

The authors are grateful to Dr. Chaofeng Lin for insightful comments and suggestions on the manuscript. This study was financially supported by the Key Sci-tech Promotion Project of State Forestry Administration, China (2010-38), the Agriculture Sci-tech System Construction Project of Ministry of Finance, China (XTG2013-36), and the Sci-tech Research Development Program of Shaanxi Province, China (2013K02-24).

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Authors and Affiliations

  1. College of Life Sciences, Northwest A&F University, Yangling, 712100, Shaanxi, China

    Fei He

  2. School of Modern Agriculture and Biotechnology, Ankang University, Ankang, 725000, Shaanxi, China

    Fei He

  3. College of Forestry, Northwest A&F University, Yangling, 712100, Shaanxi, China

    Zhongliang Zhang

  4. Agricultural Technology Promotion Center of Ankang City, Ankang, 725000, Shaanxi, China

    Ming Cui

  5. Qinba Moyu Research and Development Center, Ankang, 725000, Shaanxi, China

    Ming Cui

  6. Bureau of Konjac Langao, Langao, 725400, Shaanxi, China

    Lieping Liu

  7. College of Natural Resources and Environment, Northwest A&F University, Yangling, 712100, Shaanxi, China

    Quanhong Xue

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  1. Fei He
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  2. Zhongliang Zhang
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Correspondence to Quanhong Xue.

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The experiments comply with the current laws in China.

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The authors declare that they have no conflict of interest, and they do not have a financial relationship with the organization that sponsored the research.

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He, F., Zhang, Z., Cui, M. et al. Soft rot disease alters soil characteristics and root-associated, culturable microbial community of Amorphophallus konjac . J Gen Plant Pathol 84, 44–57 (2018). https://doi.org/10.1007/s10327-017-0759-y

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