Abstract
Here we report a novel method to extract nucleic acids for virus detection. Plant tissue in a filter paper sandwich was hit with a hammer, and the crude sap was adsorbed by the filter paper, as in hammer blotting for tissue-print immunodetection of plant viruses. Nucleic acids were extracted from the paper with a guanidine-containing buffer and purified through standard extraction and precipitation. Both viral and cellular RNAs in the extracted RNA were detectable by RT-PCR. The blots can be stored at 4 °C for more than a few days to detect viral RNA, but not cellular RNA.
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Acknowledgments
This study was supported in part by the United Graduate School of Agricultural Sciences, Ehime University, JSPS KAKENHI Grants Nos. 24658044 and 15K14664. MSA was supported by MEXT, Japan.
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Akhter, M.S., Yaeno, T., Yamaoka, N. et al. Hammer blot-mediated RNA extraction: an inexpensive, labor-saving method to extract RNA for plant virus detection. J Gen Plant Pathol 82, 268–272 (2016). https://doi.org/10.1007/s10327-016-0673-8
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DOI: https://doi.org/10.1007/s10327-016-0673-8