Abstract
Pythium species, isolated from seedlings of Glycyrrhiza uralensis with blight, were identified as P. myriotylum, P. aphanidermatum, and P. spinosum on the basis of morphological characteristics and sequences of the internal transcribed spacer regions of rDNA. In pathogenicity tests, the isolates of the three Pythium species caused blight, producing the original disease symptoms. The primary inoculum source was determined using a multiplex PCR to detect the pathogen. All the Pythium species were detected in the soils of fields with the diseased plants and in soils of adjacent field soils.
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References
Asano T, Senda M, Suga H, Kageyama K (2010) Development of multiplex PCR to detect five Pythium species related to turfgrass diseases. J Phytopathol 158:609–615
Hendrix FF Jr, Campbell WA (1973) Pythiums as plant pathogens. Annu Rev Phytopathol 11:77–98
Ishiguro Y, Asano T, Otsubo K, Suga H, Kageyama K (2013) Simultaneous detection by multiplex PCR of the high-temperature-growing Pythium species: P. aphanidermatum, P. helicoides and P. myriotylum. J Gen Plant Pathol 79:350–358
Kageyama K (2011) Characteristics of high-temperature-growing Pythium species nowadays frequently occurring disease (in Japanese). Plant Prot 65:102–106
Kageyama K, Ui T (1981) Pythium species isolated from bean plants and soils in the experimental plots of monoculture and rotation (in Japanese). Ann Phytopath Soc Japan 47:313–319
Kageyama K, Ui T (1982) Survival structure of Pythium spp. in the soils of bean fields (in Japanese). Ann Phytopath Soc Japan 48:308–313
Kumagai T, Hatakeyama Y, Henmi A, Shibano M, Kusano G (1997) Studies on cultivation and breeding Glycyrrhiza L. (1) (in Japanese). Nat Med 51:403–407
Li M, Asano T, Suga H, Kageyama K (2011) A multiplex PCR for the detection of Phytophthora nicotianae and P. cactorum, and a survey of their occurrence in strawberry production areas of Japan. Plant Dis 95:1270–1278
Morita Y, Tojo M (2007) Modifications of PARP medium using fluazinam, miconazole, and nystatin for detection of Pythium spp. in soil. Plant Dis 91:1591–1599
Nakashima C, Araki I, Ito A, Uemura S, Yamamoto A, Suzuki M (2010) Some newly recorded diseases of medical plants caused by Cercospora and its allied genera (abstract in Japanese). Jpn J Phytopathol 76:200
Ozaki K, Shibano M, Kusano G, Watanabe H (2007) Aim for production of Glycyrrhizae Radix in Japan (1) A novel cultivation method of Glycyrrhiza uralensis Fisher (in Japanese). Nat Med 61:89–92
Ozaki K, Shibano M, Kusano G, Watanabe H (2010) Aim for production of Glycyrrhizae Radix in Japan. (2) Selection of pharmaceutically fine strains from Glycyrrhiza uralensis Fisher (in Japanese). Nat Med 64:76–82
Robertson GI (1973) Pathogenicity of Pythium spp. to seeds and seedling roots. New Zeal J Agric Res 16:367–372
Shibano M, Ozaki K (2011) Aim for production of Glycyrrhizae Radix in Japan (in Japanese). Bull Osaka Univ Pharm Sci 5:59–68
Toda N, Sasaki S, Takeda O, Wei SL, Wang WQ, Wang YG, Shi H, Li G (2012) Cultivation of licorice (Glycyrrhiza uralensis) in China (Part 1): Glycyrrhizin contents in root influenced by stem cutting (in Japanese). Nat Med 66:65–70
Tojo M, Fujita Y, Awad HM, Ichitani T (1993) Preparation of Pythium inocula using bentgrass seeds for glasshouse studies (in Japanese). Proc Kansai Pl Prot 35:1–5
van der Plaats-Niterink AJ (1981) Monograph of the genus Pythium. Stud Mycol 21:1–242
Wang PH, Chang CW (2003) Detection of the low-germination-rate resting oospores of Pythium myriotylum from soil by PCR. Lett Appl Microbiol 36:157–161
Wang PH, Chung CY, Lin YS, Yeh Y (2003a) Use of polymerase chain reaction to detect the soft rot pathogen, Pythium myriotylum, in infected ginger rhizomes. Lett Appl Microbiol 36:116–120
Wang PH, Wang YT, White JG (2003b) Species-specific PCR primers for Pythium developed from ribosomal ITS1 region. Lett Appl Microbiol 37:127–132
Waterhouse GM (1967) Key to Pythium Pringsheim. Commonwealth Mycological Institute, Kew, UK. Mycol Papers 109:1–15
White TJ, Bruns T, Lee S, Taylor JW (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ (eds) PCR protocols: a guide to methods and applications. Academic Press, New York, pp 315–322
Yoshimatsu K (2012) Innovative cultivation: hydroponics of medicinal plants in the closed-type cultivation facilities. J Trad Med 29:30–34
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Ishiguro, Y., Otsubo, K., Watarai, M. et al. Seedling blight of Glycyrrhiza uralensis caused by Pythium myriotylum, P. aphanidermatum and P. spinosum and identifying primary inoculum sources using multiplex PCR detection. J Gen Plant Pathol 80, 230–236 (2014). https://doi.org/10.1007/s10327-014-0508-4
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DOI: https://doi.org/10.1007/s10327-014-0508-4