Abstract
Ustilaginoidea virens, the false smut pathogen of rice, produces false smut balls on spikelets after heading. To clarify how the fungus invades spikelets during the booting stage, we developed a fungal strain that expresses a green fluorescent protein gene and injected conidia from this strain into rice sheaths. Observations at 48 h post-inoculation showed many conidia were present on spikelet surfaces, and the conidia had germinated and the hyphae have gradually grown by 120 h post-inoculation. By 144 h, hyphae had invaded spikelets through their apices, via the small gap between the lemma and palea and had already reached all floral organs.
References
Ashizawa T, Kataoka Y (2005) Detection of Ustilaginoidea virens in rice panicles before and after heading in the field using nested-PCR technique with species-specific primers (in Japanese with English summary). Jpn J Phytopathol 71:16–19
Ashizawa T, Takahashi M, Moriwaki J, Hirayae K (2010) Quantification of the rice false smut pathogen Ustilaginoidea virens from soil in Japan using real-time PCR. Eur J Plant Pathol 128:221–232
Ashizawa T, Takahashi M, Moriwaki J, Hirayae K (2011) A refined inoculation method to evaluate false smut resistance in rice. J Gen Plant Pathol 77:10–16
Fujita Y, Sonoda R, Yaegashi H (1989) Inoculation with conidiospores of false smut fungus to rice panicles at the booting stage (in Japanese with English summary). Ann Phytopath Soc Japan 55:629–634
Ikegami H (1961) Studies on the false smut of rice VII. Analysis on the occurrence of the smut balls at the different ages (in Japanese with English summary). Res Bull Fac Agric Gifu Univ 14:27–37
Ikegami H (1963) Studies on the false smut of rice X. Invasion of chlamydospores and hyphae of the false smut fungus into rice plants. Res Bull Fac Agric Gifu Univ 18:54–60
Kepler RM, Sung G-H, Harada Y, Tanaka K, Tanaka E, Hosoya T, Bischoff JF, Spatafora JW (2012) Host jumping onto close relatives and across kingdoms by Tyrannicordyceps (Clavicipitaceae) gen. nov. and Ustilaginoidea (Clavicipitaceae). Am J Bot 99:552–561
Nakamura K, Izumiyama N, Ohtsubo K, Koiso Y, Iwasaki S, Sonoda R, Fujita Y, Yaegashi H, Sato Z (1992) Lupinosis in mice caused by ustiloxin and a crude extract of fungal culture of Ustilaginoidea virens (in Japanese with English summary). Proc Jpn Assoc Mycotoxicol 35:41–43
Prasher DC, Eckenrode VK, Ward WW, Prendergast FG, Cormier MJ (1992) Primary structure of the Aequorea victoria green-fluorescent protein. Gene 111:229–233
Satake T (1972) Circular dense-culture of rice plants in pots, the purpose of obtaining many uniform panicles of main stems (in Japanese). Jpn J Crop Sci 41:361–362
Shimomura O, Johnson FH, Saiga Y (1962) Extraction, purification and properties of aequorin, a bioluminescent protein from the luminous hydromedusan, Aequorea. J Cell Comp Physiol 59:223–239
Sonoda R, Fujita Y, Yaegashi H (1988) Distribution of the number of the false smut balls produced on rice panicle (in Japanese). Ann Rept Plant Prot North Jpn 39:92–93
Suwa M (1915) Study on Ina-kouji Part 1 (in Japanese). JAMAS (Igaku Chuo Zasshi, Japan) 13:661–689
Tanaka E, Ashizawa T, Sonoda R, Tanaka C (2008) Villosiclava virens gen. nov., com. nov., teleomorph of Ustilaginoidea virens, the causal agent of rice false smut. Mycotaxon 106:491–501
Tanaka E, Kumagawa T, Tanaka C, Koga H (2011) Simple transformation of the rice false smut fungus Villosiclava virens by electroporation of intact conidia. Mycosci 52:344–348
Watanabe S, Kumakura K, Izawa N, Nagayama K, Mitachi T, Kanamori M, Teraoka T, Arie T (2007) Mode of action of Trichoderma asperellum SKT-1, a biocontrol agent against Gibberella fujikuroi. J Pestic Sci 32:222–228
White JF Jr, Sullivan R, Moy M, Patel R, Duncan R (2000) An overview of problems in the classification of plant-parasitic Clavicipitaceae. Stud Mycol 45:95–105
Zhang Z, Du X, Chai R, Mao X, Qui H, Wang Y, Wang J, Sun G (2006) Agrobacterium tumefaciens-mediated transformation of the pathogen of Ustilaginoidea virens (in Chinese). Chin J Rice Sci 20:440–442
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Ashizawa, T., Takahashi, M., Arai, M. et al. Rice false smut pathogen, Ustilaginoidea virens, invades through small gap at the apex of a rice spikelet before heading. J Gen Plant Pathol 78, 255–259 (2012). https://doi.org/10.1007/s10327-012-0389-3
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DOI: https://doi.org/10.1007/s10327-012-0389-3