Abstract
Virus-like symptoms—red ringspots on stems and leaves, circular blotches or pale spots on fruit—were found on commercial highbush blueberry (Vaccinium corymbosum) cultivars Blueray, Weymouth, Duke and Sierra in Japan. In PCR testing, single DNA fragments were amplified from total nucleic acid samples of the diseased blueberry bushes using primers specific to Blueberry red ringspot virus (BRRV). Sequencing analysis of the amplified products revealed 95.7–97.7% nucleotide sequence identity with the BRRV genome. This paper is the first report of blueberry red ringspot disease caused by BRRV in Japan.
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References
Caruso FL, Ramsdell DC (1995) Compendium of blueberry and cranberry diseases. Disease compendium series. APS Press, St. Paul
Fauquet CM, Mayo MA, Maniloff J, Desselberger U, Ball LA (eds) (2005) Virus taxonomy. In: 8th report of the international committee on taxonomy of viruses. Elsevier Academic Press, Amsterdam
Gasic K, Hernandez A, Korban SS (2004) RNA extraction from different apple tissues rich in polyphenols and polysaccharides for cDNA library construction. Plant Mol Biol Rep 22:437a–437g
Gillett JM, Ramsdell DC (1988) Blueberry red ringspot virus. AAB Descriptions of plant viruses, #327, AAB, Wellesbourne, Warwick, UK
Glasheen BM, Polashock JJ, Lawrence DM, Gillett JM, Ramsdell DC, Vorsa N, Hillman BI (2002) Cloning, sequencing, and promoter identification of Blueberry red ringspot virus, a member of the family Caulimoviridae with similartities to the “Soybean chlorotic mottle-like” genus. Arch Virol 147:2169–2186
Hepp RF, Converse RH (1987) Blueberry red ringspot virus detection in crude sap of highbush blueberry plants. Plant Dis 71:536–539
Hutchinson MT, Varney EH (1954) Ringspot-A virus disease of cultivated blueberry. Plant Dis Rep 38:260–262
Jaswal AS (1990) Occurrence of blueberry leaf mottle, blueberry shoestring, tomato ringspot and tobacco ringspot viruses in eleven halfhigh blueberry clones grown in New Brunswick, Canada. Can Plant Dis Survey 70:113–117
Paulechova K (1972) The occurrence of red ringspot virus disease of blueberry in Czechoslovakia. Biologia 27:791–793
Salzman RA, Fujita T, Zhu-Salzman K, Hasegawa PM, Bressan RA (1999) An improved RNA isolation method for plant tissues containing high levels of phenolic compounds or carbohydrates. Plant Mol Biol Rep 17:11–17
Acknowledgments
We thank Mr. Yoshiyuki Kume for his support in field investigations in Chiba prefecture.
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The nucleotide sequence data reported in this paper are available in the GenBank/EMBL/DDBJ database as accessions AB469884 to AB469893 for BRRV isolates from Japan.
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Isogai, M., Ishii, K., Umemoto, S. et al. First report of blueberry red ringspot disease caused by Blueberry red ringspot virus in Japan. J Gen Plant Pathol 75, 140–143 (2009). https://doi.org/10.1007/s10327-009-0145-5
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DOI: https://doi.org/10.1007/s10327-009-0145-5