Abstract
Shell fish processing industry is very common in coastal areas. While processing, only the meat is taken, the head and shells are discarded as waste. On an average, the sea food industry produces 80,000 tons of waste per year. The sheer amount of waste makes degradation a slow process causing accumulation of waste over a period of time. A very simple and effective solution to this environmental hazard is the recycling of shell waste to commercially viable products like chitin. Chitosan is the N-acetyl derivative of chitin obtained by N-deacetylation. Chitosan is widely used in food and bioengineering industries for encapsulation of active food ingredients, enzyme immobilization, as a carrier for controlled drug delivery, in agriculture as a plant growth promoter. Chitosan is also a defense elicitor and an antimicrobial agent. Chitosan has interesting properties such as biodegradability, biocompatibility, bioactivity, nontoxicity and polycationic nature. This review presents structural characteristics and physicochemical properties of chitosan. The methods of preparation of chitosan nanoparticles are detailed. Applications of chitosan nanoparticles are discussed. Applications include drug delivery, encapsulation, antimicrobial agent, plant growth-promoting agent and plant protector.


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Abdel-Aziz HM, Hasaneen MN, Omer AM (2016) Nano chitosan-NPK fertilizer enhances the growth and productivity of wheat plants grown in sandy soil. Span J Agric Res 14(1):0902
Abdou ES, Osheba AS, Sorour MA (2012) Effect of chitosan and chitosan-nanoparticles as active coating on microbiological characteristics of fish fingers. Int J Appl Sci Technol 2(7):158–169
Agbodjato NA, Noumavo PA, Adjanohoun A, Agbessi L, Baba-Moussa L (2016) Synergistic effects of plant growth promoting rhizobacteria and chitosan on in vitro seeds germination, greenhouse growth, and nutrient uptake of maize (zea mays l.). Biotechnol Res Int 2016:1–11
Agrawal GK, Rakwal R, Tamogami S, Yonekura M, Kubo A, Saji H (2002) Chitosan activates defense/stress response (s) in the leaves of oryza sativa seedlings. Plant Physiol Biochem 40(12):1061–1069
Ahmed RA, Fekry A (2013) Preparation and characterization of a nanoparticles modified chitosan sensor and its application for the determination of heavy metals from different aqueous media. Int J Electrochem Sci 8(3):6692–6708
Aiping Z, Jianhong L, Wenhui Y (2006) Effective loading and controlled release of camptothecin by O-carboxymethylchitosan aggregates. Carbohydr Polym 63(1):89–96
Algam S, Xie G, Li B, Yu S, Su T, Larsen J (2010) Effects of paenibacillus strains and chitosan on plant growth promotion and control of ralstonia wilt in tomato. J Plant Pathol 92(3):593–600
Ali SW, Rajendran S, Joshi M (2011) Synthesis and characterization of chitosan and silver loaded chitosan nanoparticles for bioactive polyester. Carbohydr Polym 83(2):438–446
Allen MJ, Schoonmaker JE, Bauer TW, Williams PF, Higham PA, Yuan HA (2004) Preclinical evaluation of a poly (vinyl alcohol) hydrogel implant as a replacement for the nucleus pulposus. Spine 29(5):515–523
Arafat A, Samad SA, Huq D, Moniruzzaman M, Masum SM (2015) Textile dye removal from wastewater effluents using chitosan–ZnO nanocomposite. J Text Sci Eng 5(3):1
Aranaz I, Mengabar M, Harris R, Is Paos, Miralles B, Acosta N, Heras A et al (2009) Functional characterization of chitin and chitosan. Curr Chem Biol 3(2):203–230
Badawy MEI, Rabea EI (2011) A biopolymer chitosan and its derivatives as promising antimicrobial agents against plant pathogens and their applications in crop protection. Int J Carbohydr Chem 2011:1–29
Baxter A, Dillon M, Anthony Taylor KD, Roberts GAF (1992) Improved method for ir determination of the degree of n-acetylation of chitosan. Int J Biol Macromol 14(3):166–169
Brunel F, Varon L, David L, Domard A, Delair T (2008) A novel synthesis of chitosan nanoparticles in reverse emulsion. Langmuir 24(20):11370–11377
Calvo P, Remunanae-Lopez C, Vila-Jato JL, Alonso MJ (1997) Novel hydrophilic chitosan polyethylene oxide nanoparticles as protein carriers. J Appl Polym Sci 63(1):125–132
Chamnanmanoontham N, Pongprayoon W, Pichayangkura R, Roytrakul S, Chadchawan S (2015) Chitosan enhances rice seedling growth via gene expression network between nucleus and chloroplast. Plant Growth Regul 75(1):101–114
Chandra S, Chakraborty N, Dasgupta A, Sarkar J, Panda K, Acharya K (2015) Chitosan nanoparticles: a positive modulator of innate immune responses in plants. Sci Rep 5:1–14
Cheba BA (2011) Chitin and chitosan: marine biopoly-mers with unique properties and versatile applications. Glob J Biotechnol Biochem 6(3):149–153
Chen DH, Leu JC, Huang TC (1994) Transport and hydrolysis of urea in a reactor–separator combining an anion-exchange membrane and immobilized urease. J Chem Technol Biotechnol 61(4):351–357
Cho M, No H, Prinyawiwatkul W (2008) Chitosan treatments affect growth and selected quality of sunflower sprouts. J Food Sci 73(1):70–77
Chowdappa P, Gowda S (2013) Nanotechnology in crop protection: status and scope. Pest Manag Hortic Ecosyst 19(2):131–151
Chowdappa P, Kumar SM, Lakshmi MJ, Upreti K (2013) Growth stimulation and induction of systemic resistance in tomato against early and late blight by bacillus subtilis OTPB1 or trichoderma harzianum OTPB3. Biol Control 65(1):109–117
Chowdappa P, Gowda S, Chethana C, Madhura S (2014) Antifungal activity of chitosan–silver nanoparticle composite against colletotrichum gloeosporioides associated with mango anthracnose. Afr J Microbiol Res 8(17):1803–1812
Cicek S, Nadaroglu H (2015) The use of nanotechnology in the agriculture. Adv Nano Res 3(4):207–223
Corradini E, De Moura MR, Mattoso LHC (2010) A preliminary study of the incorparation of NPK fertilizer into chitosan nanoparticles. Express Polym Lett 4(8):509–515
Dahlan KZHM, Hashim K, Bahari K, Mahmod M, Yaacob N, Talip N et al (2010) Application of radiation degraded chitosan as plant growth promoter. A pilot scale production and field trial study of radiation processed chitosan as plant growth promoter for rice crops. International Atomic Energy Agency
de Paz LEC, Resin A, Howard KA, Sutherland DS, Wejse PL (2011) Antimicrobial effect of chitosan nanoparticles on streptococcus mutans biofilms. Appl Environ Microbiol 77(11):3892–3895
De TK, Ghosh PK, Maitra A, Sahoo SK (1999) Process for the preparation of highly monodispersed polymeric hydrophilic nanoparticles: Google Patents
Dehaghi SM, Rahmanifar B, Moradi AM, Azar PA (2014) Removal of permethrin pesticide from water by chitosan–zinc oxide nanoparticles composite as an adsorbent. J Saudi Chem Soc 18(4):348–355
Divya K, Rebello S, Jisha MS (2014) A simple and effective method for extraction of high purity chitosan from shrimp shell waste. In: Proceedings of the international conference on advances in applied science and environmental engineering-ASEE
Divya K, Vijayan S, George TK, Jisha M (2017) Antimicrobial properties of chitosan nanoparticles: mode of action and factors affecting activity. Fibers Polym 18(2):221–230
Du W-L, Niu S-S, Xu Y-L, Xu Z-R, Fan C-L (2009) Antibacterial activity of chitosan tripolyphosphate nanoparticles loaded with various metal ions. Carbohydr Polym 75(3):385–389
Dzung NA, Khanh VTP, Dzung TT (2011) Research on impact of chitosan oligomers on biophysical characteristics, growth, development and drought resistance of coffee. Carbohydr Polym 84(2):751–755
Einbu A, Vayrum KM (2008) Characterization of chitin and its hydrolysis to glcnac and glcn. Biomacromol 9(7):1870–1875
El-Shabouri MH (2002) Positively charged nanoparticles for improving the oral bioavailability of cyclosporin-A. Int J Pharm 249(1):101–108
Erbacher P, Zou S, Bettinger T, Steffan A-M, Remy J-S (1998) Chitosan-based vector/DNA complexes for gene delivery: biophysical characteristics and transfection ability. Pharm Res 15(9):1332–1339
Falcon-Rodriguez AB, Costales D, Cabrera JC, Martinez-Tellez MA (2011) Chitosan physico–chemical properties modulate defense responses and resistance in tobacco plants against the oomycete phytophthora nicotianae. Pestic Biochem Physiol 100(3):221–228
Fang H, Huang J, Ding L, Li M, Chen Z (2009) Preparation of magnetic chitosan nanoparticles and immobilization of laccase. J Wuhan Univ Technol Mater Sci Ed 24(1):42–47
Fernandez-Urrusuno R, Calvo P, Remuan-Lopez C, Vila-Jato JL, Alonso MJ (1999) Enhancement of nasal absorption of insulin using chitosan nanoparticles. Pharm Res 16(10):1576–1581
Franca EF, Lins RD, Freitas LCG, Straatsma TP (2008) Characterization of chitin and chitosan molecular structure in aqueous solution. J Chem Theory Comput 4(12):2141–2149
Geng X, Kwon O-H, Jang J (2005) Electrospinning of chitosan dissolved in concentrated acetic acid solution. Biomaterials 26(27):5427–5432
Ghadi A, Mahjoub S, Tabandeh F, Talebnia F (2014) Synthesis and optimization of chitosan nanoparticles: potential applications in nanomedicine and biomedical engineering. Casp J Int Med 5(3):156
Ghadi A, Tabandeh F, Mahjoub S, Mohsenifar A, Roshan FT, Alavije RS (2015) Fabrication and characterization of core-shell magnetic chitosan nanoparticles as a novel carrier for immobilization of Burkholderia cepacia lipase. J Oleo Sci 64(4):423–430
Ghormade V, Deshpande MV, Paknikar KM (2011) Perspectives for nano-biotechnology enabled protection and nutrition of plants. Biotechnol Adv 29(6):792–803
Grenha A (2010) Chitosan nanoparticles: a survey of preparation methods. J Drug Target 20(4):291–300
Grillo R, Pereira AE, Nishisaka CS, de Lima R, Oehlke K, Greiner R, Fraceto LF (2014) Chitosan/tripolyphosphate nanoparticles loaded with paraquat herbicide: an environmentally safer alternative for weed control. J Hazard Mater 278:163–171
Gupta AK, Naregalkar RR, Vaidya VD, Gupta M (2007) Recent advances on surface engineering of magnetic iron oxide nanoparticles and their biomedical applications. Nanomedicine (Lond) 2(1):23–29
Hadwiger L, Klosterman S, Choi J (2002) The mode of action of chitosan and its oligomers in inducing plant promoters and developing disease resistance in plants. Adv Chitin Sci 5:452–457
Haider S, Park S-Y (2009) Preparation of the electrospun chitosan nanofibers and their applications to the adsorption of Cu (II) and Pb(II) ions from an aqueous solution. J Membr Sci 328(1):90–96
Hajirasouliha MJM, Soheili F, Naja Fabadi (2012) Effect of novel chitosan nanoparticle coating on pot harvest qualities of strawberry. In: Proceedings of the 4th international conference on nanostructures
Hatton RA, Miller AJ, Silva SRP (2008) Carbon nanotubes: a multi-functional material for organic optoelectronics. J Mater Chem 18:1183–1192
Hernandez-Lauzardo AN, Velaizquez-del Valle MG, Guerra-Sanchez MG (2011) Current status of action mode and effect of chitosan against phytopathogens fungi. Afr J Microbiol Res 5(25):4243–4247
Hirano S, Yamaguchi Y, Kamiya M (2002) Novel N-saturated-fatty-acyl derivatives of chitosan soluble in water and in aqueous acid and alkaline solutions. Carbohydr Polym 48(2):203–207
Honary S, Ghajar K, Khazaeli P, Shalchian P (2011) Preparation, characterization and antibacterial properties of silver–chitosan nanocomposites using different molecular weight grades of chitosan. Trop J Pharm Res 10(1):69–74
Hosseini F, SadighianS Hosseini-Monfared F, Mahmoodi NM (2016) Dye removal and kinetics of adsorption by magnetic chitosan nanoparticles. Desalin Water Treat 57(51):24378–24386
Hu Z, Zhang J, Chan W, Szeto Y (2006) The sorption of acid dye onto chitosan nanoparticles. Polymer 47(16):5838–5842
Hu B, Pan C, Sun Y, Hou Z, Ye H, Zeng X (2008) Optimization of fabrication parameters to produce chitosan tripolyphosphate nanoparticles for delivery of tea catechins. J Agric Food Chem 56(16):7451–7458
Huang YC, Li RY (2014) Preparation and characterization of antioxidant nanoparticles composed of chitosan and fucoidan for antibiotics delivery. Mar Drugs 12(8):4379–4398
Huang L, Cheng X, Liu C, Xing K, Zhang J, Sun G, Chen X et al (2009) Preparation, characterization, and antibacterial activity of oleic acid-grafted chitosan oligosaccharide nanoparticles. Front Biol China 4(3):321–327
Ilium L (1998) Chitosan and its use as a pharmaceutical excipient. Pharm Res 15(9):1326–1331
Ingle A, Gade A, Pierrat S, Sonnichsen C, Rai M (2008) Mycosynthesis of silver nanoparticles using the fungus fusarium acuminatum and its activity against some human pathogenic bacteria. Curr Nanosci 4(2):141–144
Janes KA, Fresneau MP, Marazuela A, Fabra A, Alonso MJ (2001) Chitosan nanoparticles as delivery systems for doxorubicin. J Control Release 73(2):255–267
Jang K-I, Lee HG (2008) Stability of chitosan nanoparticles for l-ascorbic acid during heat treatment in aqueous solution. J Agric Food Chem 56(6):1936–1941
Jayakumar R, Menon D, Manzoor K, Nair SV, Tamura H (2010a) Biomedical applications of chitin and chitosan based nanomaterials: a short review. Carbohydr Polym 82(2):227–232
Jayakumar R, Prabaharan M, Nair S, Tamura H (2010b) Novel chitin and chitosan nanofibers in biomedical applications. Biotechnol Adv 28(1):142–150
Jeon SJ, Oh M, Yeo W-S, Galvao KN, Jeong KC (2014) Underlying mechanism of antimicrobial activity of chitosan microparticles and implications for the treatment of infectious diseases. PLoS ONE 9(3):92723
Jia Y-T, Gong J, Gu X-H, Kim H-Y, Dong J, Shen X-Y (2007) Fabrication and characterization of poly (vinyl alcohol)/chitosan blend nanofibers produced by electrospinning method. Carbohydr Polym 67(3):403–409
Jonassen H, Kjoniksen AL, Hiorth M (2012) Stability of chitosan nanoparticles cross-linked with tripolyphosphate. Biomacromol 13(11):3747–3756
Kananont N, Pichyangkura R, Chanprame S, Chadchawan S, Limpanavech P (2010) Chitosan specificity for the in vitro seed germination of two dendrobium orchids (asparagales: Orchidaceae). Sci Hortic 124(2):239–247
Kasaai MR (2009) Various methods for determination of the degree of n-acetylation of chitin and chitosan: a review. J Agric Food Chem 57(5):1667–1676
Kashyap PL, Xiang X, Heiden P (2015) Chitosan nanoparticle based delivery systems for sustainable agriculture. Int J Biol Macromol 77:36–51
Kaur P, Thakur R, Choudhary A (2012) An in vitro study of the antifungal activity of silver/chitosan nanoformulations against important seed borne pathogens. Int J Sci Technol Res 1:83–86
Kaur P, Choudhary A, Thakur R (2013) Synthesis of chitosan–silver nanocomposites and their antibacterial activity. Int J Sci Eng Res 4:869–872
Kong M, Chen X, Xue Y, Liu C, Yu L, Ji Q et al (2008) Preparation and antibacterial activity of chitosan microshperes in a solid dispersing system. Front Mater Sci China 2(2):214–220
Kumar MNVR (1999) Chitin and chitosan fibres: a review. Bull Mater Sci 22(5):905–915
Kumar S, Koh J (2012) Physiochemical and optical study of chitosan–terephthaldehyde derivative for biomedical applications. Int J Biol Macromol 51(5):1167–1172
Kumirska J, Mg Czerwicka, Kaczyaski Z, Bychowska A, Brzozowski K, Thaming J, Stepnowski P (2010) Application of spectroscopic methods for structural analysis of chitin and chitosan. Mar Drugs 8(5):1567–1636
Kumirska J, Weinhold MX, Thaming J, Stepnowski P (2011) Biomedical activity of chitin/chitosan based materials influence of physicochemical properties apart from molecular weight and degree of n-acetylation. Polymers 3(4):1875–1901
Kwok KC, Koong LF, Chen G, McKay G (2014) Mechanism of arsenic removal using chitosan and nanochitosan. J Colloid Interface Sci 416:1–10
Lin SB, Chen SH, Peng KC (2009) Preparation of antibacterial chito-oligosaccharide by altering the degree of deacetylation of β-chitosan in a Trichoderma harzianum chitinase-hydrolysing process. J Sci Food Agric 89(2):238–244
Liu C-G, Desai KGH, Chen X-G, Park H-J (2005) Preparation and characterization of nanoparticles containing trypsin based on hydrophobically modified chitosan. J Agric Food Chem 53(5):1728–1733
Liu H, Tian W, Li B, Wu G, Ibrahim M, Tao Z, Sun G et al (2012) Antifungal effect and mechanism of chitosan against the rice sheath blight pathogen, Rhizoctonia solani. Biotechnol Lett 34(12):2291–2298
Lopez-Leon T, Carvalho ELS, Seijo B, Ortega-Vinuesa JL, Bastos-Gonzailez D (2005) Physicochemical characterization of chitosan nanoparticles: electrokinetic and stability behavior. J Colloid Interface Sci 283(2):344–351
Ma Y, Liu P, Si C, Liu Z (2010) Chitosan nanoparticles: preparation and application in antibacterial paper. J Macromol Sci Part B 49(5):994–1001
Mahdavi B, Rahimi A (2013) Seed priming with chitosan improves the germination and growth performance of ajowan (carum copticum) under salt stress. EurAsia J Biosci 7:69–76
Makhluf S, Dror R, Nitzan Y, Abramovich Y, Jelinek R, Gedanken A (2005) Microwave assisted synthesis of nanocrystalline mgo and its use as a bacteriocide. Adv Funct Mater 15(10):1708–1715
Malmiri HJ, Jahanian MAG, Berenjian A (2012) Potential applications of chitosan nanoparticles as novel support in enzyme immobilization. Am J Biochem Biotechnol 8(4):203–219
Manikandan A, Sathiyabama M (2016) Preparation of chitosan nanoparticles and its effect on detached rice leaves infected with pyricularia grisea. Int J Biol Macromol 84:58–61
Manjusha EM, Mohan JC, Manzoor K, Nair SV, Tamura H, Jayakumar R (2010) Folate conjugated carboxymethyl chitosan–manganese doped zinc sulphide nanoparticles for targeted drug delivery and imaging of cancer cells. Carbohydr Polym 80:414–420
Min B-M, Lee SW, Lim JN, You Y, Lee TS, Kang PH, Park WH (2004) Chitin and chitosan nanofibers: electrospinning of chitin and deacetylation of chitin nanofibers. Polymer 45(21):7137–7142
Mohammadpour Dounighi N, Eskandari R, Avadi M, Zolfagharian H, Mir Mohammad Sadeghi A, Rezayat M (2012) Preparation and in vitro characterization of chitosan nanoparticles containing Mesobuthus eupeus scorpion venom as an antigen delivery system. J Venom Anim Toxins Incl Trop Dis 18(1):44–52
Mondal M, Rana MIK, Dafader N, Haque M (2011) Effect of foliar application of chitosan on growth and yield in Indian spinach. J Agrofor Environ 5(1):99–102
Mondal M, Malek M, Puteh A, Ismail M, Ashrafuzzaman M, Naher L (2012) Effect of foliar application of chitosan on growth and yield in okra. Aust J Crop Sci 6(5):918
Muzzarelli RAAJC, Gooday GW (1986) Chitin in nature and technology. Plenum Publishing Corporation, New York
Namasivayam SKR, Roy EA (2013) Enhanced antibiofilm activity of chitosan stabilized chemogenic silver nanoparticles against Escherichia coli. Int J Sci Res 2013:591
Nge KL, Nwe N, Chandrkrachang S, Stevens WF (2006) Chitosan as a growth stimulator in orchid tissue culture. Plant Sci 170(6):1185–1190
Nguyen T, Dzung T, Cuong P (2014) Assessment of antifungal activity of turmeric essential oil-loaded chitosan nanoparticles. J Chem Biol Phys Sci 4:2347–2356
Nguyen TV, Nguyen TTH, Wang S-L, Vo TPK, Nguyen AD (2016) Preparation of chitosan nanoparticles by tpp ionic gelation combined with spray drying, and the antibacterial activity of chitosan nanoparticles and a chitosan nanoparticle–amoxicillin complex. Res Chem Intermed 2016:1–11
Niwa T, Takeuchi H, Hino T, Kunou N, Kawashima Y (1993) Preparations of biodegradable nanospheres of water-soluble and insoluble drugs with d, l-lactide/glycolide copolymer by a novel spontaneous emulsification solvent diffusion method, and the drug release behavior. J Control Release 25(1):89–98
Ohkawa K, Cha D, Kim H, Nishida A, Yamamoto H (2004) Electrospinning of chitosan. Macromol Rapid Commun 25(18):1600–1605
Olivera S, Muralidhara HB, Venkatesh K, Guna VK, Gopalakrishna K, Kumar Y (2016) Potential applications of cellulose and chitosan nanoparticles/composites in wastewater treatment: a review. Carbohydr Polym 153:600–618
Onsosyen E, Skaugrud O (1990) Metal recovery using chitosan. J Chem Technol Biotechnol 49(4):395–404
Ottey MH, Varum KM, Smidsra DO (1996) Compositional heterogeneity of heterogeneously deacetylated chitosans. Carbohydr Polym 29(1):17–24
Panyam J, Labhasetwar V (2003) Biodegradable nanoparticles for drug and gene delivery to cells and tissue. Adv Drug Deliv Rev 55(3):329–347
Park JK, Chung MJ, Choi HN, Park YI (2011) Effects of the molecular weight and the degree of deacetylation of chitosan oligosaccharides on antitumor activity. Int J Mol Sci 12(1):266–277
Peppas NA, Huang Y (2004) Nanoscale technology of mucoadhesive interactions. Adv Drug Deliv Rev 56(11):1675–1687
Perera U, Rajapakse N (2013) Chitosan nanoparticles: preparation, characterization, and applications. In: Kim SK (ed) Seafood processing by-products: trends and applications. Springer, New York, pp 371–387
Pilon L, Spricigo PC, Miranda M, Moura MR, Assis OBG, Mattoso LHC, Ferreira MD (2015) Chitosan nanoparticle coatings reduce microbial growth on fresh-cut apples while not affecting quality attributes. Int J Food Sci Technol 50(2):440–448
Plainsirichai M, Leelaphatthanapanich S, Wongsachai N (2014) Effect of chitosan on the quality of rose apples (syzygium agueum alston) cv. Tabtim chan stored at an ambient temperature. APCBEE Procedia 8:317–322
Postma J, Stevens LH, Wiegers GL, Davelaar E, Nijhuis EH (2009) Biological control of pythium aphanidermatum in cucumber with a combined application of lysobacter enzymogenes strain 3.1 T8 and chitosan. Biol Control 48(3):301–309
Puvvada YS, Vankayalapati S, Sukhavasi S (2012) Extraction of chitin from chitosan from exoskeleton of shrimp for application in the pharmaceutical industry. Int Curr Pharm J 1(9):258–263
Qi L, Xu Z, Jiang X, Hu C, Zou X (2004) Preparation and antibacterial activity of chitosan nanoparticles. Carbohydr Res 339(16):2693–2700
Raafat D, Von Bargen K, Haas A, Sahl H-G (2008) Insights into the mode of action of chitosan as an antibacterial compound. Appl Environ Microbiol 74(12):3764–3773
Rajalakshmi A, Krithiga N, Jayachitra A (2013) Antioxidant activity of the chitosan extracted from shrimp exoskeleton. Middle East J Sci Res 16(10):1446–1451
Rajasree R, Rahate K (2013) An overview on various modifications of chitosan and it’s applications. Int J Pharm Sci Res 4(11):4175
Rajendran R, Abirami M, Prabhavathi P, Premasudha P, Kanimozhi B, Manikandan A (2015) Biological treatment of drinking water by chitosan based nanocomposites. Afr J Biotechnol 14(11):930–936
Ravishankar Rai V, Jamuna Bai A (2011) Nanoparticles and their potential application as antimicrobials, science against microbial pathogens: communicating current research and technological advances. In: Méndez-Vilas A (ed), Formatex, Microbiology Series, No. 3, Vol. 1. Spain, pp 197–209
Rekso GT (2008) Development of radiation degraded chitosan as plant growth promoter and its economic evaluation. JAEA CONF 2008-9
Rinaudo M (2006) Chitin and chitosan: properties and applications. Prog Polym Sci 31(7):603–632
Rodriguez A, Ramirez M, Cardenas R, Hernandez A, Velazquez M, Bautista S (2007) Induction of defense response of Oryza sativa L. against Pyricularia grisea (cooke) Sacc. By treating seeds with chitosan and hydrolyzed chitosan. Pestic Biochem Physiol 89(3):206–215
Saavedra GM, Figueroa NE, Poblete LA, Cherian S, Figueroa CR (2016) Effects of preharvest applications of methyl jasmonate and chitosan on postharvest decay, quality and chemical attributes of fragaria chiloensis fruit. Food Chem 190:448–453
Saharan V, Mehrotra A, Khatik R, Rawal P, Sharma S, Pal A (2013) Synthesis of chitosan based nanoparticles and their in vitro evaluation against phytopathogenic fungi. Int J Biol Macromol 62:677–683
Sailaja A, Amareshwar P, Chakravarty P (2011) Different techniques used for the preparation of nanoparticles using natural polymers and their application. Int J Pharm Pharm Sci 3(Suppl 2):45–50
Salachna P, Zawadzińska A (2014) Effect of chitosan on plant growth, flowering and corms yield of potted freesia. J Ecol Eng 15(3):97–102
Sarwar A, Katas H, Zin NM (2014) Antibacterial effects of chitosan–tripolyphosphate nanoparticles: impact of particle size molecular weight. J Nanopart Res 16(7):2517
Seyedi SM, Anvaripour B, Motavassel M, Jadidi N (2013) Comparative cadmium adsorption from water by nanochitosan and chitosan. Int J Eng Innov Technol 2(9):145–148
Shahidi F, Arachchi JKV, Jeon Y-J (1999) Food applications of chitin and chitosans. Trends Food Sci Technol 10(2):37–51
Sharma S, Sharma S (2013) Synthesis, characterization and determination of encapsulation efficiency of chitosan nanoparticles for terbinafine. Indo Am J Pharm Res 3(12):1564–1567
Shi B, Shen Z, Zhang H, Bi J, Dai S (2011a) Exploring N-imidazolyl-O-carboxymethyl chitosan for high performance gene delivery. Biomacromol 13(1):146–153
Shi LE, Tang ZX, Yi Y, Chen JS, Xiong WY, Ying GQ (2011b) Immobilization of nuclease p1 on chitosanmicro-spheres. Chem Biochem Eng Q 25(1):83–88
Shiraishi S, Imai T, Otagiri M (1993) Controlled release of indomethacin by chitosan-polyelectrolyte complex: optimization and in vivo/in vitro evaluation. J Control Release 25(3):217–225
Sims DC, Butler PE, Casanova R, Lee BT, Randolph MA, Lee WA, Yaremchuk MJ et al (1996) Injectable cartilage using polyethylene oxide polymer substrates. Plast Reconstr Surg 98(5):843–850
Sivakami M, Gomathi T, Venkatesan J, Jeong H-S, Kim S-K, Sudha P (2013) Preparation and characterization of nano chitosan for treatment wastewaters. Int J Biol Macromol 57:204–212
Sonia T, Sharma CP (2011) Chitosan and its derivatives for drug delivery perspective. Adv Polym Sci 243:23–53
Sudarshan NR, Hoover DG, Knorr D (1992) Antibacterial action of chitosan. Food Biotechnol 6(3):257–272
Sun K, Li Z (2011) Preparations, properties and applications of chitosan based nanofibers fabricated by electrospinning. Express Polym Lett 5(4):342–361
Thanou M, Nihot M, Jansen M, Verhoef JC, Junginger H (2001) Mono-N-carboxymethyl chitosan (MCC), a polyampholytic chitosan derivative, enhances the intestinal absorption of low molecular weight heparin across intestinal epithelia in vitro and in vivo. J Pharm Sci 90(1):38–46
Tiyaboonchai W (2003) Chitosan nanoparticles: a promising system for drug delivery. Naresuan Univ J 11(3):51–66
Van Toan N, Hanh TT (2013) Application of chitosan solutions for rice production in vietnam. Afr J Biotechnol 12(4):382–384
Van SN, Minh HD, Anh DN (2013) Study on chitosan nanoparticles on biophysical characteristics and growth of robusta coffee in green house. Biocatal Agric Biotechnol 2(4):289–294
Vauthier CDC, Chauvierre C, Brigger I, Couvreur P (2003) Drug delivery to resistant tumors: the potential of poly(alkyl cyanoacrylate) nanoparticles. J Control Release 93:151–160
Vazquez-Duhalt R, Tinoco R, D’Antonio P, Topoleski LDT, Payne GF (2001) Enzyme conjugation to the polysaccharide chitosan: smart biocatalysts and biocatalytic hydrogels. Bioconjug Chem 12(2):301–306
Wang X, Xing B (2007) Importance of structural makeup of biopolymers for organic contaminant sorption. Environ Sci Technol 41(10):3559–3565
Wang M, Chen Y, Zhang R, Wang W, Zhao X, Du Y, Yin H (2015) Effects of chitosan oligosaccharides on the yield components and production quality of different wheat cultivars (triticum aestivum l.) in northwest china. Field Crop Res 172:11–20
Wei D, Sun W, Qian W, Ye Y, Ma X (2009) The synthesis of chitosan-based silver nanoparticles and their antibacterial activity. Carbohydr Res 344(17):2375–2382
Whitesides GM (2003) The’right’size in nanobiotechnology. Nat Biotechnol 21(10):1161–1165
Xie W, Xu P, Liu Q (2001) Antioxidant activity of water-soluble chitosan derivatives. Bioorg Med Chem Lett 11(13):1699–1701
Xing K, Shen X, Zhu X, Ju X, Miao X, Tian J, Qin S et al (2016) Synthesis and in vitro antifungal efficacy of oleoyl-chitosan nanoparticles against plant pathogenic fungi. Int J Biol Macromol 82:830–836
Yacob N, Mahmud M, Talip N, Hashim K, Harun AR, Zaman K, Dahlan H (2013) Degradation of chitosan for rice crops application. Nucl Sci Tech 24(S1):10301–S010301
Yang K, Xu N-S, Su WW (2010) Co-immobilized enzymes in magnetic chitosan beads for improved hydrolysis of macromolecular substrates under a time-varying magnetic field. J Biotechnol 148(2):119–127
Yen MT, Mau JL (2007) Selected physical properties of chitin prepared from shiitake stipes. LWT Food Sci Technol 40(3):558–563
Yen MT, Yang JH, Mau JL (2008) Antioxidant properties of chitosan from crab shells. Carbohydr Polym 74(4):840–844
Yen MT, Yang JH, Mau JL (2009) Physicochemical characterization of chitin and chitosan from crab shells. Carbohydr Polym 75(1):15–21
Yien L, Zin NM, Sarwar A, Katas H (2012) Antifungal activity of chitosan nanoparticles and correlation with their physical properties. Int J Biomater 2012:1–9
Yoshii F, Zhanshan Y, Isobe K, Shinozaki K, Makuuchi K (1999) Electron beam crosslinked PEO and PEO/PVA hydrogels for wound dressing. Radiat Phys Chem 55(2):133–138
Zhang Y, Zhang X, Ding R, Zhang J, Liu J (2011) Determination of the degree of deacetylation of chitosan by potentiometric titration preceded by enzymatic pretreatment. Carbohydr Polym 83(2):813–817
Zhao LM, Shi LE, Zhang ZL, Chen JM, Shi DD, Yang J, Tang ZX (2011) Preparation and application of chitosan nanoparticles and nanofibers. Braz J Chem Eng 28(3):353–362
Zolghadri S, Jalilian AR, Yousefnia H, Bahrami-Samani A, Shirvani-Arani S, Mazidi M, Akhlaghi M, Ghannadi-Maragheh M (2010) Production and quality control of 166Ho-Chitosan for therapeutic applications. Iran J Nucl Med 18(2):1–8
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Divya, K., Jisha, M.S. Chitosan nanoparticles preparation and applications. Environ Chem Lett 16, 101–112 (2018). https://doi.org/10.1007/s10311-017-0670-y
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DOI: https://doi.org/10.1007/s10311-017-0670-y