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Effect of stimulation of anterior hypothalamic area on urinary bladder function of the anesthetized rat

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Abstract.

The hypothalamus is a key area for the integration of the autonomic features of affective behavior. Hypothalamic defence area (HDA) stimulation evokes major cardiorespiratory changes as well as modifications of general autonomic activity both in the anesthetized and conscious animal. Micturition is due to an increase in pelvic parasympathetic activity and, in the cat, the anterior hypothalamus has been implicated in urinary bladder control with the demonstration of a dorsolateral vesicoconstrictor pathway and a ventromedial inhibitory pathway. In this study we have investigated the effect of electrical and chemical stimulation of the HDA on bladder pressure and contractions in rat. Female rats (n = 15) were anesthetized, paralyzed and ventilated artificially. Arterial blood pressure, heart rate, urinary bladder pressure and pelvic nerve activity were recorded. HDA was electrically (1 ms, 100 Hz, 5–10 s train at intensities up to 150 µA) and chemically (sodium glutamate, 50nl, 2mM) stimulated. For statistical analysis the t–test was used, data were expressed as mean ± SEM. Values of t were taken as significant when p < 0.05.HDA stimulation at 100–150 µA evoked changes of both mean blood pressure (mBP) and bladder pressure (BlP). However, stimulation at < 30µA allowed a distinction within HDA of two different regions, at the same antero–posterior and lateral level, but separated 100–150µm in depth, which evoked differential effects on blood pressure and urinary bladder pressure. Results show that low intensity stimulation at ventral sites evoked a significant increase of mBP (from 102 ± 5.9 to 127 ± 8.6mmHg, n = 10, p < 0.0001) with little changes of BlP (from 12 ± 2.2 to 16 ± 2.9cmH2O, n = 10, p < 0.0005), whilst at more dorsal sites significant increases of BlP were elicited (from 12 ± 8.3 to 38 ± 4.6cmH2O,n = 10, p < 0.0001) with only a small rise of mBP (from 102 ± 6.2 to 111 ± 9.8mmHg, n = 10, p < 0.005). Glutamate injections at dorsal sites evoked a rise of BlP (from 11 ± 2.2 to 30 ± 3.0cmH2O (n = 5; p < 0.0001) with small changes in BP, whilst at ventral sites (n = 4) glutamate microinjections evoked changes in BP but not of BlP. In conclusion stimulation at different sites within HDA can elicit separate changes in BP and BlP.

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References

  1. VC, Hilton SM, Zybrozyna A (1960) Active muscle vasodilation produced by stimulation of the brainstem: its significance in the defence reaction. J Physiol 154:491–513

    CAS  PubMed  Google Scholar 

  2. Bagshaw EV, Evans MH (1986) Measurement of current spread from microelectrodes when stimulating within the central nervous system. Exp Brain Res 25:391–400

    Google Scholar 

  3. Blok BFM, de Weerd H, Holstege G (1995) Ultrastructural evidence for a paucity of projections from lumbosacral cord to the pontine micturition center or M-region in the cat: a new concept for the organization of the micturition reflex with the periaqueductal grey as a central relay. J Comp Neurol 359:300–309

    CAS  PubMed  Google Scholar 

  4. De Groat WC, Booth AM, Yoshimura N (1993) Neurophysiology of micturition and its modifications in animal models of human disease. In:Maggi CA (ed) “Nervous control of the urogenital system”. Harwood Academic Publishers, London

  5. Ding Y-Q, Zheng H-H, Gong L-W, Lu Y, Zhao H, Qin B-Z (1997) Direct projections from lumbosacral spinal cord to Barrington’s nucleus in the rat: a special reference to micturition reflex. J Comp Neurol 389:149–160

    Google Scholar 

  6. Eliasson S, Folkow B, Lindgren P, Uvnas B (1951) Activation of sympathetic vasodilator nerves to skeletal muscles in the cat by hypothalamic stimulation. Acta Physiol Scand 23: 333–351

    Google Scholar 

  7. Enoch DM, Kerr FWL (1967) Hypothalamic vasopressor and vesicopressor pathways. II. Anatomic study of their course and connections. Arch Neurol 16:307–320

    Google Scholar 

  8. Enoch DM, Kerr FWL (1967) Hypothalamic vasopressor and vesicopressor pathways. I. Functional studies. Arch Neurol 16:290–306

    CAS  PubMed  Google Scholar 

  9. Feigl E, Johansson B, Lofving B (1964) Renal vasoconstrition and the defence reaction. Acta Physiol Scand 62:429–435

    CAS  PubMed  Google Scholar 

  10. Gjone R (1966) Excitatory and inhibitory bladder responses to stimulation of limbic, diencephalic and mesencephalic structures in the cat. Acta Physiol Scand 59:337–349

    Google Scholar 

  11. Hilton SM (1982) The defence-arousal system and its relevance for circulatory and respiratory control. J Exp Biol 100:159–174

    Google Scholar 

  12. Hunsperger RW (1956) Affektreationen auf elektrische Reizung im Hirnstamm der Katze. Helv Physiol Pharmacol Acta 14:70–92

    Google Scholar 

  13. Jordan D (1990) Autonomic changes in afective behavior. In: Loewy AD, Spyer KM (eds) “Central Regulation of Autonomic Function”. Oxford University Press

  14. Kylstra PH, Lisander B (1970) Differentiated interaction between the hypothalamic defence reaction and baroreceptor reflexes. II. Effects on aortic blood flow as related to work load on the left ventricle. Acta Physiol Scand 78:386–392

    Google Scholar 

  15. Marson L (1997) Identification of central nervous system neurons that innervate the bladder body, the bladder base, or external urethral sphincter of female rats: a transneuronal study using pseudirabies virus. J Comp Neurol 389:584–602

    Article  CAS  PubMed  Google Scholar 

  16. Maseri A (1985) Mixed angina pectoris. Am J Cardiol 56:30

    PubMed  Google Scholar 

  17. Morrison JBF (1987) Bladder control: role of higher levels of the central nervous system. In: Torrens M, Morrison JBF (eds) “The Physiology of the Urogenital System”. Springer-Verlag

  18. Nathan PW (1976) The central nervous connections of the bladder. In: Williams DI, Chrisholm GC (eds) “Scientific Foundations of Urology”, vol II. Heinemann

  19. Noto H, Roppolo JR, Steers WD, de Groat WC (1991) Electrophysiological analysis of the ascending and descending components of the micturition reflex pathway in the rat. Brain Res 549:95–105

    Google Scholar 

  20. Paxinos G, Watson C (1997) The rat brain in stereotaxic co-ordinates (5th edition).Academic Press, London

  21. Rocha I, Burnstock G, Spyer KM (2001) Effect on urinary bladder function of the activation of putative purinoceptors of brainstem areas. Aut Neurosci: basic and clin 88:6–15

    Google Scholar 

  22. Rocha I, Rosário LB, Oliveira EI, Barros MA, Silva-Carvalho L (2003) Enhancement of carotid chemoreceptor reflex and cardiac chemosensitive reflex in the acute phase of myocardial infarction in the anaesthetized rabbit. Basic Res Cardiol 98(3):175–180

    CAS  Google Scholar 

  23. Rosen A (1961) Augmented cardiac contraction, heart rate acceleration and skeletal muscle vasodilation produced by hypothalamic stimulation in the cats. Acta Physiol Scand 52:291–308

    PubMed  Google Scholar 

  24. Tang PC (1955) Levels of brainstem and diencephalon controlling the micturition reflex. J Neurophysiol 18:583–595

    PubMed  Google Scholar 

  25. Tang PC, Ruch TC (1956) Localization of brainstem and diencephalic areas controlling the micturition reflex. J Comp Neurol 106:213–245

    PubMed  Google Scholar 

  26. Valentino RJ, Pavcovich LA, Hirata H (1995) Evidence for corticotrophin hormone projections from Barrington’s nucleus to periaqueductal grey and dorsal motor nucleus of the vagus in the rat. J Comp Neurol 363:402–22

    Google Scholar 

  27. Yardley CP, Hilton SM (1986) The hypothalamic and brainstem areas from which cardiovascular and behavioral components of the defence reaction are elicited in the rat. JANS 15:227–244

    Article  Google Scholar 

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Authors and Affiliations

  1. Instituto de Fisiologia, Faculdade de Medicina de Lisboa, Av. Prof. Egas Moniz , 1649-028, Lisbon, Portugal

    I. Rocha & L. Silva-Carvalho

  2. Dept. of Physiology, Royal Free and University College School of Medicine, London, UK

    K. M. Spyer

Authors
  1. I. Rocha
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  2. L. Silva-Carvalho
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  3. K. M. Spyer
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Correspondence to L. Silva-Carvalho.

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Rocha, I., Silva-Carvalho, L. & Spyer, K.M. Effect of stimulation of anterior hypothalamic area on urinary bladder function of the anesthetized rat. Clin Auton Res 14, 264–269 (2004). https://doi.org/10.1007/s10286-004-0212-0

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  • DOI: https://doi.org/10.1007/s10286-004-0212-0

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