Skip to main content

Advertisement

SpringerLink
Log in

Comparison of the sealing ability amongst orthograde apical plugs of mineral trioxide aggregate plus, mineral trioxide aggregate repair HP, and Biodentine after root resection: a bacterial leakage study

  • Original Article
  • Published:
Odontology Aims and scope Submit manuscript

Abstract

This study aimed to analyse the effect of root-end resection on the sealing ability of orthograde apical plugs of three root-end filling materials (MTA Repair HP, Biodentine, MTA Plus) using the bacterial leakage method and compare it with the retrograde root-end filling technique. Eighty-four extracted mandibular premolar teeth with single root and single straight canals were selected and randomly divided into two main experimental groups (n = 36) depending on the apical plug placement technique and a control: Group I: orthograde technique, followed by root-end resection; Group II: retrograde root-end filling technique; one control Group III (n = 12): obturation with gutta-percha, followed by root-end resection. Experimental groups were further subdivided into three subgroups A, B, and C (n = 12) depending on the three different root-end filling materials used. An apical plug of 3 mm thickness was obtained in both the experimental groups. The apical sealing ability was evaluated with the dual-chamber bacterial leakage method. Statistical analysis was performed using the Kaplan–Meier survival analysis test. By the end of 30 days, the occurrence of turbidity indicating bacterial leakage was 75% in MTA Repair HP, 83.3% in Biodentine, and 91.7% in MTA Plus samples. This trend of turbidity was similar in both orthograde and retrograde technique. The control group showed turbidity in all the samples (100%). More MTA Repair HP samples (25%) survived the 30 days observation period with no bacterial leakage compared to Biodentine (16.7%) and MTA Plus (8.3%). The sealing ability of already set root-end filling materials placed through the orthograde technique did not deteriorate after root-end resection. Also among three root-end filling materials, MTA Repair HP presented the lowest bacterial microleakage followed by Biodentine and MTA Plus, respectively.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

Data availability

The data supporting the findings of this study are available upon request from the corresponding author.

References

  1. Moradi S, Disfani R, Ghazvini K, Lomee M. Sealing ability of orthograde MTA and CEM cement in apically resected roots using bacterial leakage method. Iran Endod J. 2013;8:109–13.

    PubMed  PubMed Central  Google Scholar 

  2. Montellano AM, Schwartz SA, Beeson TJ. Contamination of tooth-colored mineral trioxide aggregate used as a root-end filling material: a bacterial leakage study. J Endod. 2006;32:452–5.

    Article  PubMed  Google Scholar 

  3. Medeiros PL, Bernardineli N, Cavenago BC, Torres SA, Duarte M, Bramante CM, et al. Sealing ability of MTA, CPM, and MBPc as root-end filling materials: a bacterial leakage study. J Appl Oral Sci. 2016;24:148–52.

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  4. Gondim E, Zaia AA, Gomes BPFA, Ferraz CCR, Teixeira FB, Souza-Filho FJ. Investigation of the marginal adaptation of root-end filling materials in root-end cavities prepared with ultrasonic tips. Int Endod J. 2003;36:491–9.

    Article  PubMed  CAS  Google Scholar 

  5. Moradi S, Disfani R, Lomee M, Naghavi N. Effect of root resection on sealing ability of orthograde apical plugs of mineral trioxide aggregate and calcium-enriched mixture. J Dent (Tehran). 2014;11:447–54.

    PubMed  Google Scholar 

  6. Habibi M, Ghoddusi J, Habibi A, Mohtasham N. Healing process following application of set or fresh mineral trioxide aggregate as a root-end filling material. Eur J Dent. 2011;5:19–23.

    Article  PubMed  PubMed Central  Google Scholar 

  7. Yildirim T, Er K, Taşdemir T, Tahan E, Buruk K, Serper A. Effect of smear layer and root-end cavity thickness on apical sealing ability of MTA as a root-end filling material: a bacterial leakage study. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2010;109:67–72.

    Article  Google Scholar 

  8. Torabinejad M, Ung B, Kettering JD. In vitro bacterial penetration of coronally unsealed endodontically treated teeth. J Endod. 1990;16:566–9.

    Article  PubMed  CAS  Google Scholar 

  9. Gorni FG, Gagliani MM. The outcome of endodontic retreatment: a 2-year follow-up. J Endod. 2004;30:1–4.

    Article  PubMed  Google Scholar 

  10. Timpawat S, Amornchat C, Trisuwan WR. Bacterial coronal leakage after obturation with three root canal sealers. J Endod. 2001;27:36–9.

    Article  PubMed  CAS  Google Scholar 

  11. Barthel CR, Moshonov J, Shuping G, Orstavik D. Bacterial leakage versus dye leakage in obturated root canals. Int Endod J. 1999;32:370–5.

    Article  PubMed  CAS  Google Scholar 

  12. Sim TP, Knowles JC, Ng YL, Shelton J, Gulabivala K. Effect of sodium hypochlorite on mechanical properties of dentine and tooth surface strain. Int Endod J. 2001;34(2):120–32.

    Article  PubMed  CAS  Google Scholar 

  13. Hachmeister DR, Schindler WG, Walker WA 3rd, Thomas DD. The sealing ability and retention characteristics of mineral trioxide aggregate in a model of apexification. J Endod. 2002;28:386–90.

    Article  PubMed  Google Scholar 

  14. Hong ST, Bae KS, Baek SH, Kum KY, Lee W. Microleakage of accelerated mineral trioxide aggregate and Portland cement in an in vitro apexification model. J Endod. 2008;34:56–8.

    Article  PubMed  Google Scholar 

  15. Kim S, Kratchman J. Modern endodontic surgery concepts and practice: a review. J Endod. 2006;32:601–23.

    Article  PubMed  Google Scholar 

  16. Post LK, Xavier CB, Demarco FF. Sealing ability of MTA and amalgam in different root-end preparations and resection bevel angles: an in vitro evaluation using marginal dye leakage. Braz Dent J. 2010;21:416–9.

    Article  PubMed  Google Scholar 

  17. Peter AG, Figdor D, Martin J. Apical dentin permeability and microleakage associated with root end resection and retrograde filling. J Endod. 1994;20:22–6.

    Article  Google Scholar 

  18. Lamb EL, Loushine RJ, Weller N, Kimborugh WF, Pashley DH. Effect of root resection on the apical sealing ability of mineral trioxide aggregate. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2003;95:732–5.

    Article  PubMed  Google Scholar 

  19. Wuchenich G, Meadows D, Torabinejad M. A comparison between two root endpreparation techniques in human cadavers. J Endod. 1994;20:279–82.

    Article  PubMed  CAS  Google Scholar 

  20. Engel TK, Steiman HR. Preliminary investigation of ultrasonic root-end preparation. J Endod. 1995;21:443–8.

    Article  PubMed  CAS  Google Scholar 

  21. Navarre SW, Steiman R. Root-end fracture during retropreparation: a comparison between zirconium nitride-coated and stainless steel microsurgical ultrasonic instruments. J Endod. 2002;28:330–2.

    Article  PubMed  Google Scholar 

  22. Al-Hezaimi K, Naghshbandi J, Oglesby S, Simon JH, Rotstein I. Human saliva penetration of root canals obturated with two types of mineral trioxide aggregate cements. J Endod. 2005;31:453–6.

    Article  PubMed  Google Scholar 

  23. Tavares L, Cintra A, Benetti F, Olinta I, Queiroz DA, Maria J, et al. Cytoxicity, biocompatibility, and biomineralization of the new high-plasticity MTA material. J Endod. 2017;18:1–5.

    Google Scholar 

  24. Andelin WE, Browning DF, Hsu GR, Roland DD, Torabinejad M. Microleakage of resected MTA. J Endod. 2002;28:573–4.

    Article  PubMed  Google Scholar 

  25. Milani AS, Sahar S, Borna Z. Evaluating the effect of resection on the sealing ability of MTA and CEM cement. Iran Endod J. 2012;7:134–8.

    PubMed  PubMed Central  Google Scholar 

Download references

Acknowledgements

We would like to offer our sincere thanks to Dr. Sandesh N for his assistance with the statistics used in the research.

Author information

Authors and Affiliations

  1. Department of Conservative Dentistry and Endodontics, Sri Aurobindo College of Dentistry, Indore, Madhya Pradesh, India

    Chhaya Harinkhere, Pallav Mahesh Patni, Pradeep Jain, Swadhin Raghuwanshi, Sanket Hans Pandey & Sakshi Bilaiya

Authors
  1. Chhaya Harinkhere
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Pallav Mahesh Patni
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Pradeep Jain
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Swadhin Raghuwanshi
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Sanket Hans Pandey
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Sakshi Bilaiya
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

Chhaya Harinkhere: conceptualization, methodology, visualization. Pallav Mahesh Patni: data curation, supervision, validation. Pradeep Jain: methodology, visualization, reviewing. Swadhin Raghuwanshi: supervision, writing—editing. Sanket Hans Pandey: software, validation, writing—reviewing. Sakshi Bilaiya: supervision, validation, writing—reviewing and editing.

Corresponding author

Correspondence to Chhaya Harinkhere.

Ethics declarations

Conflict of interest

None.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Harinkhere, C., Patni, P.M., Jain, P. et al. Comparison of the sealing ability amongst orthograde apical plugs of mineral trioxide aggregate plus, mineral trioxide aggregate repair HP, and Biodentine after root resection: a bacterial leakage study. Odontology 112, 364–371 (2024). https://doi.org/10.1007/s10266-023-00847-4

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10266-023-00847-4

Keywords

Search

Navigation