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Pulpal outcomes in orthodontic tooth movement in diabetes mellitus

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Abstract

Diabetes mellitus impairs angiogenesis and tissue reorganization during orthodontic tooth movement (OTM). Thus, this study evaluated pulpal outcomes in orthodontic tooth movement through metabolic changes in diabetes. Male Wistar rats were used, and the in vivo study design consisted of four groups (n = 10/group): C—non-diabetic animals not subjected to orthodontic tooth movement; D—diabetic animals not subjected to orthodontic tooth movement; OTM—non-diabetic animals subjected to orthodontic tooth movement; and D + OTM—diabetic animals subjected to orthodontic tooth movement. In addition, the pulps of the distovestibular root (DV) and mesiovestibular root (MV) were assessed by histomorphometric analyses and immunoexpression of the RANKL/OPG system. Pulpal analysis of the MV root showed an increase in blood vessels in diabetic animals. Inflammatory infiltrate and fibroblastic cells were elevated in diabetic animals with tooth movement in the DV and MV roots. In the DV and MV roots, diabetic rats with OTM showed a reduction in birefringent collagen fibers. The immunostaining for RANKL was higher in the pulp tissue of OTM in diabetic and non-diabetic animals. It was concluded that the pulp tissue has less adaptive and repair capacity during OTM in diabetes. Orthodontic strength can alter the inflammatory processes in the pulp.

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Acknowledgements

This work was supported by the National Council for Scientific and Technological Development–CAPES/PNPD (Process no 23038.008192/2013‐01) and the Hermínio Ometto Foundation.

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  1. Postgraduate Program in Orthodontics, FHO–University Center of the Hermínio Ometto Foundation, Dr. Maximiliano Baruto Ave. 500, Araras, São Paulo, 13607-339, Brazil

    Milton Santamaria-Jr & Evandro Raphael Alves do Nascimento

  2. Postgraduate Program in Biomedical Sciences, FHO–University Center of the Hermínio Ometto Foundation, Dr. Maximiliano Baruto Ave. 500, Araras, São Paulo, 13607-339, Brazil

    Milton Santamaria-Jr, Leonardo Bagne, Bruno Calsa & Marcelo Augusto Marretto Esquisatto

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  1. Milton Santamaria-Jr
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Santamaria-Jr, M., do Nascimento, E.R.A., Bagne, L. et al. Pulpal outcomes in orthodontic tooth movement in diabetes mellitus. Odontology 109, 921–929 (2021). https://doi.org/10.1007/s10266-021-00609-0

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