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A universal adhesive incorporating antimicrobial peptide nisin: effects on Streptococcus mutans and saliva-derived multispecies biofilms

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Abstract

For purpose of enhancing the antibacterial activity of a universal adhesive, the antimicrobial peptide nisin was incorporated into Single Bond Universal and its antibacterial effect on Streptococcus mutans monospecific biofilms and saliva-derived multispecies biofilms was studied. Nisin was incorporated into Single Bond Universal and the antibacterial activity was examined by confocal laser scanning microscopy (CLSM), reverse transcription-quantitative polymerase chain reaction (qRT-PCR), phenol–sulfuric acid method and lactate dehydrogenase enzymatic method. The bonding properties were tested by microtensile bond strength (μTBS) and degree of conversion (DC). Data were analyzed by one-way analysis of variance (ANOVA) and least significant difference multiple comparison tests (P < 0.05). The Single Bond Universal incorporated with 3% (w/v) nisin could significantly inhibit the growth of the S. mutans monospecific biofilms (P< 0.01) and decrease the expression of genes related to extracellular polysaccharide (EPS) synthesis (gtfB, gtfC, gtfD and spaP) and acidogenicity (ldh) (P < 0.05). 3% (w/v) nisin-incorporated Single Bond Universal could also inhibit the growth of saliva-derived multispecies biofilms and decrease the excretion of EPS and lactic acid ( P< 0.05). μTBS and DC of 3% (w/v) nisin-incorporated Single Bond Universal did not deteriorate obviously (P > 0.05). In conclusion, 3% (w/v) nisin-incorporated Single Bond Universal substantially inhibited the growth of both S. mutans monospecific and saliva-derived multispecies biofilms without compromising the bonding properties.

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Acknowledgements

This study was supported by the National Natural Science Foundation of China (Grant No. 81873712) and the Science Foundation of Guangzhou (Grant No. 201904010057).

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  1. Manduo Zhao and Yang Qu authors contributed equally to this work.

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  1. Guangdong Provincial Key Laboratory of Stomatology, Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-sen University, 56 Lingyuanxi Road, Yuexiu District, Guangzhou, Guangdong Province, China

    Manduo Zhao, Yang Qu, Jia Liu, Sui Mai & Lisha Gu

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  1. Manduo Zhao
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Zhao, M., Qu, Y., Liu, J. et al. A universal adhesive incorporating antimicrobial peptide nisin: effects on Streptococcus mutans and saliva-derived multispecies biofilms. Odontology 108, 376–385 (2020). https://doi.org/10.1007/s10266-019-00478-8

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