Abstract
∆8-sphingolipid desaturase catalyzes the C8 desaturation of a long chain base, which is the characteristic structure of various complex sphingolipids. The genes of 20 ∆8-sphingolipid desaturases from 12 plants were identified and functionally detected by using Saccharomyces cerevisiae system to elucidate the relationship between the biochemical function and evolution of this enzyme. Results showed that the 20 genes all can encode a functional ∆8-sphingolipid desaturase, which catalyzes different ratios of two products, namely, 8(Z) and 8(E)-C18-phytosphingenine. The coded enzymes could be divided into two groups on the basis of biochemical functions: ∆8-sphingolipid desaturase with a preference for an E-isomer product and ∆8-sphingolipid desaturase with a preference for a Z-isomer product. The conversion rate of the latter was generally lower than that of the former. Phylogenetic analysis revealed that the 20 desaturases could also be clustered into two groups, and this grouping is consistent with that of the biochemical functions. Thus, the biochemical function of ∆8-sphingolipid desaturase is correlated with its evolution. The two groups of ∆8-sphingolipid desaturases could arise from distinct ancestors in higher plants. However, they might have initially evolved from ∆8-sphingolipid desaturases in lower organisms, such as yeasts, which can produce E-isomer products only. Furthermore, almost all of the transgenic yeasts harboring ∆8-sphingolipid desaturase genes exhibit an improvement in aluminum tolerance. Our study provided new insights into the biochemical function and evolution of ∆8-sphingolipid desaturases in plants.
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Abbreviations
- LCB:
-
Long chain base
- HSP:
-
High score pairs
- PCR:
-
Polymerase chain reaction
- ORF:
-
Open reading frame
- UPLC:
-
Ultra-performance liquid chromatography
- t18:0:
-
C18-phytosphinganine
- t18:18Z :
-
8(Z)-C18-phytosphinganine
- t18:18E :
-
8(E)-C18-phytosphinganine
- HPLC/ESI–MS/MS:
-
High-performance liquid chromatotraphy/electrospray ionization tandem mass spectrometry
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Acknowledgments
This work was supported by Grants from the National Natural Science foundation of China (31300202 and 31470334).
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Fig. S1 Alignment of the deduced amino acid sequences of various ∆8-sphingolipid desaturase genes isolated in this study. Only partial sequences representing conserved regions are shown. Numbers on the right denote the number of amino acid residues. Characteristic regions of the N-terminal cytb 5 motif are labeled by dots, and the three conserved histidine boxes are underlined (PDF 8370 kb)
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Li, SF., Zhang, GJ., Zhang, XJ. et al. Genes encoding Δ8-sphingolipid desaturase from various plants: identification, biochemical functions, and evolution. J Plant Res 129, 979–987 (2016). https://doi.org/10.1007/s10265-016-0838-8
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DOI: https://doi.org/10.1007/s10265-016-0838-8