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DNA amplification on chromosome 13q31.1 correlated with poor prognosis in colorectal cancer

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Abstract

Colorectal cancer is a leading cause of cancer deaths worldwide. Genetic markers involved in prognosis of colorectal cancer are still being elucidated. In this study, genetic alterations associated with prognosis of colorectal cancer were determined using arbitrarily primed polymerase chain reaction (AP-PCR) and analyzed quantitatively by real-time PCR. Seven different DNA sequences, mapped on chromosomes 13q31.1, 9q31.1, 1q24, 4q31.3, 10q21, 11q13.4, and 13q13.3, were identified. Among these sequences, seven cases (23%) harbored DNA amplification in chromosome 13q31.1, and 9 (29%) and 7 (23%) presented genetic alterations in chromosome 1q24 and 11q13.4, respectively. Multivariate analysis showed that only DNA amplification in chromosome 13q31.1 was associated with poor survival among patients with colorectal cancer, with median survival time for chromosome 13q31.1 amplification versus no amplification of 64 versus 268 weeks (P = 0.001). This genetic alteration may have a prognostic role in colorectal cancer.

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References

  1. Parkin DM, Bray F, Ferlay J, Pisani P (2005) (2002) Global cancer statistics, CA cancer. J Clin 55:74–108

    Google Scholar 

  2. Srisukho S, Srivatanakul P, Sumitsawan Y (2007) Colon and rectum. In: Khuhaprema T et al (eds) Cancer in Thailand vol IV, 1998–2000. Bangkok Medical Publisher, Bangkok, pp 34–35

    Google Scholar 

  3. Ilyas M, Straub J, Tomlinson IPM, Bodmer WF (1999) Genetic pathways in colorectal and other cancers. Eur J Cancer 35:1986–2002

    Article  PubMed  CAS  Google Scholar 

  4. Baker SJ, Preisinger AC, Jessup JM et al (1990) p53 Mutations occur in combination with 17p allelic deletions as late events in colorectal tumorigenesis. Cancer Res 50:7717–7722

    PubMed  CAS  Google Scholar 

  5. Galiatsatos P, Foulkes WD (2006) Familial adenomatous polyposis. Am J Gastroenterol 101:385–398

    Article  PubMed  Google Scholar 

  6. Chung DC (2000) The genetic basis of colorectal cancer: insights into critical pathways of tumorigenesis. Gastroenterol 119:854–865

    Article  CAS  Google Scholar 

  7. Chaksangchaichot P, Punyarit P, Petmitr S (2007) Novel hMSH2, hMSH6 and hMLH1 gene mutations and microsatellite instability in sporadic colorectal cancer. J Cancer Res Clin Oncol 133:65–70

    Article  PubMed  CAS  Google Scholar 

  8. Micthell RJ, Farrington SM, Dunlop MG, Campbell H (2002) Mismatch repair genes hMLH1 and hMSH2 and colorectal cancer: a HuGE review. Am J Epidemiol 156:885–902

    Article  Google Scholar 

  9. Welsh J, McClelland M (1990) Fingerprinting genomes using PCR with arbitrary primers. Nucleic Acids Res 18:7213–7218

    Article  PubMed  CAS  Google Scholar 

  10. Williams JG, Kubelik AR, Livak KJ, Rafalski JA, Tingey SV (1990) DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res 18:6531–6535

    Article  PubMed  CAS  Google Scholar 

  11. Navarro JM, Jorcano JL (1999) The use of arbitrarily primed polymerase chain reaction in cancer research. Electrophoresis 20:283–290

    Article  PubMed  CAS  Google Scholar 

  12. Rosai J (1996) Ackerman’s surgical pathology, 8th edn, vol II, Mosby Year Book Inc. USA

  13. Miller SA, Dykes DD, Polesky HF (1988) A simple salting out procedure for extracting DNA from human nucleated cells. Nucleic Acids Res 16:1215

    Article  PubMed  CAS  Google Scholar 

  14. Sealee P, Wongkham S, Bhudhisawasdi V, Sripa B, Chariyalertsak S, Petmitr S (2008) Loss on chromosome 5q34 is associated with poor prognosis in hepatocellular carcinoma. J Cancer Res Clin Oncol 134:1135–1141

    Article  Google Scholar 

  15. Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real- time quantitative PCR and the 2(Delta Delta C(T)) method. Methods 25:402–408

    Article  PubMed  CAS  Google Scholar 

  16. Jass JR (2006) Colorectal cancer: a multipathway diseases. Crit Rev Oncog 12:273–287

    PubMed  Google Scholar 

  17. Kallioniemi A, Kallioniemi OP, Sudar D et al (1992) Comparative genomic hybridization for molecular cytogenetic analysis in solid tumors. Science 258:818–821

    Article  PubMed  CAS  Google Scholar 

  18. Oga A, Kawauchi S, Izumi H, Ping LX, Furuya T, Susuki K (2002) New perspectives for tumor pathology provided by comparative genomic hybridization. In J Clin Oncol 7:133–137

    Article  CAS  Google Scholar 

  19. Liu XP, Kawauchi S, Oga A, Sato T, Ikemoto K, Ikeda E, Sasaki K (2007) Chromosomal aberrations detected by comparative chromosomal hybridization predict outcome in patients with colorectal carcinoma. Oncol Rep 17:261–267

    PubMed  CAS  Google Scholar 

  20. de Juan C, Iniesta P, Vega FJ et al (1998) Prognostic value of genomic damage in non-small-cell lung cancer. Br J Cancer 77:1971–1977

    Article  PubMed  Google Scholar 

  21. Maeda T, Jikko A, Hiranuma H, Fuchihata H (1999) Analysis of genome instability in squamous cell carcinoma of the head and neck using the random amplified polymorphic DNA method. Cancer Lett 138:183–188

    Article  PubMed  CAS  Google Scholar 

  22. Chuensumran U, Wongkham S, Pairojkul C, Chauin S, Petmitr S (2007) Prognostic value of DNA alterations on chromosome 17p13.2 for intrahepatic cholangiocarcinoma. World J Gastroenterol 13:2986–2991

    PubMed  CAS  Google Scholar 

  23. Lin Y-W, Sheu J-C, Chen C-H et al (1999) Loss of heterozygosity at chromosome 13q in hepatocellular carcinoma: identification of three independent regions. Eur J Cancer 35:1730–1734

    Article  PubMed  CAS  Google Scholar 

  24. Sabbir MG, Roy A, Mandal S, Dam A, Roychoudhury S, Panda CK (2006) Deletion mapping of chromosome 13q in head and neck squamous cell carcinoma in Indian patients: correlation with prognosis of the tumour. Int J Exp Path 87:151–161

    Article  CAS  Google Scholar 

  25. Gordon AT, Brinkschmidt C, Anderson J et al (2000) A novel and consistent amplicon at 13q31 associated with alveolar rhabdomyosarcoma. Genes Chromosomes Cancer 28:220–226

    Article  PubMed  CAS  Google Scholar 

  26. Schmidt H, Bartel F, Kappler M et al (2005) Gains of 13q are correlated with a poor prognosis in liposarcoma. Mod Pathol 18:638–644

    Article  PubMed  CAS  Google Scholar 

  27. Yu W, Inoue J, Imoto I, Matsuo Y, Karpas A, Inazawa J (2003) GPC5 is a possible target for the 13q31–32 amplification detected in lymphoma cell line. J Hum Genet 48:331–335

    PubMed  CAS  Google Scholar 

  28. Ota A, Tagawa H, Karnan S, Tsuzuki S, Karpas A, Kira S, Yoshida Y, Seto M (2004) Identification and characterization of a novel gene, C13orf25, as a target for 13q31–32 amplification in malignant lymphoma. Cancer Res 64:3087–3095

    Article  PubMed  CAS  Google Scholar 

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Acknowledgments

This research project was supported by Mahidol University. The authors thank Professor Prapon Wilairat, Department of Biochemistry, Faculty of Science, and Mr. Paul Adams, Faculty of Tropical Medicine, Mahidol University for correcting the manuscript.

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Authors and Affiliations

  1. Department of Tropical Nutrition and Food Science, Faculty of Tropical Medicine, Mahidol University, 420/6 Ratchawithi Road, Bangkok, 10400, Thailand

    Tawiwan Sareeboot & Songsak Petmitr

  2. Institute of Pathology, Phramongkutklao Medical Center, Bangkok, Thailand

    Phaibul Punyarit

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  1. Tawiwan Sareeboot
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  2. Phaibul Punyarit
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  3. Songsak Petmitr
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Correspondence to Songsak Petmitr.

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Sareeboot, T., Punyarit, P. & Petmitr, S. DNA amplification on chromosome 13q31.1 correlated with poor prognosis in colorectal cancer. Clin Exp Med 11, 97–103 (2011). https://doi.org/10.1007/s10238-010-0107-4

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  • DOI: https://doi.org/10.1007/s10238-010-0107-4

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