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The mRNA and protein expression of A-kinase anchor proteins 13 in human colorectal cancer

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Abstract

There was no literature reporting the relationship between AKAP13 and colorectal carcinoma. This study is aim to investigate the expression and role of AKAP13 in human colorectal cancers. This study investigated 94 pair-matched human colorectal cancers and adjacent normal mucosa, as well as 36 adenomas, of which mRNA expression of AKAP13 was detected by relative Quantitative-Real-Time RT-PCR and protein expression by immunohistochemical staining. AKAP13 gene was upregulated in colorectal cancer group by 2.259 times compared to control group without significant difference (P = 0.081), and no expression was detected in adenoma by RT-PCR. The positive expression rate of AKAP13 protein in colorectal carcinoma (52.3%) was significantly higher than those in adenoma (9.1%) and normal tissue (34.7%) (P = 0.006) by immunohistochemical staining. Either the mRNA or protein expressions of AKAP13 were correlated with histological types and differentiation grade (P < 0.05). Our results suggest AKAP13 protein may be related to the carcinogenesis of human colorectal cancer. However, more deeply and larger scale research are required to prove the correlation.

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Acknowledgments

This study was granted by National Natural Science Foundation of China (No. 30600591) and China Medical Board of New York Inc. (No. CMB 96636). The authors thank Professor Kai-Xuan Yang (West China Second Hospital, Sichuan University) for her professional pathologic technology support.

Conflict of interest statement

The authors declare that they have no conflict of interest related to the publication of this manuscript.

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Authors and Affiliations

  1. Department of General Surgery & Institute of Digestive Surgery and State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, No.37 Guo Xue Xiang St., 610041, Chengdu, Sichuan Province, China

    Jian-Kun Hu, Ling Wang, Yuan Li, Kun Yang, Peng Zhang, Xin-Zu Chen, Rong Wang & Zong-Guang Zhou

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  1. Jian-Kun Hu
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  2. Ling Wang
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  3. Yuan Li
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Correspondence to Zong-Guang Zhou.

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Hu, JK., Wang, L., Li, Y. et al. The mRNA and protein expression of A-kinase anchor proteins 13 in human colorectal cancer. Clin Exp Med 10, 41–49 (2010). https://doi.org/10.1007/s10238-009-0065-x

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