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Microvascular hyperpermeability and thrombosis induced by light/dye treatment

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Abstract

To investigate microvascular hyperpermeability and thrombosis induced by photodynamic therapy or light/dye treatment, we quantified the initiation time for thrombus formation, thrombus growth rate, and the time for the microvessel occlusion in post-capillary venules of rat mesenteries. Under similar light/dye treatments, we also measured the microvessel hydraulic conductivity (Lp) and solute permeability (P) to TRITC-BSA (bovine serum albumin), respectively, in the same type of microvessels as for thrombosis. Under an irradiation power of 0.37 mW/mm2, thrombus was initiated in 3.8 ± 0.4 min, its growth rate was 3.9 ± 0.3% of the vessel mid-plane area/min, and the microvessels were completely occluded in 29.3 ± 2.2 min (SE, n = 8). Under the same irradiation power, Lp and P increased gradually, reaching a plateau in 3–5 min. At the plateau, Lp had increased to 2.2 ± 0.2 times (n = 11), while P had increased to 4.1 ± 0.7 (n = 7) times their baseline values, respectively. Neither Lp nor P increased further after longer time exposure (up to 30 min). Comparison of the measured Lp and P data with predictions from a mathematical model for the inter-endothelial cleft suggests that an almost complete depletion of the glycocalyx layer at the luminal surface of the endothelium might be one of the structural mechanisms by which the light/dye increases microvascular permeability and induces thrombosis.

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Authors and Affiliations

  1. Department of Biomedical Engineering, The City College of the City University of New York, Steinman Hall Room 402, 140th Street & Convent Ave., New York, NY, 10031, USA

    Qin Liu, Min Zeng & Bingmei M. Fu

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  1. Qin Liu
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  2. Min Zeng
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  3. Bingmei M. Fu
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Correspondence to Bingmei M. Fu.

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Liu, Q., Zeng, M. & Fu, B.M. Microvascular hyperpermeability and thrombosis induced by light/dye treatment. Biomech Model Mechanobiol 10, 235–247 (2011). https://doi.org/10.1007/s10237-010-0230-x

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  • DOI: https://doi.org/10.1007/s10237-010-0230-x

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