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Role of CD44 expression in non-tumor tissue on intrahepatic recurrence of hepatocellular carcinoma

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Abstract

Background

CD44 is well known to be one of the cancer stem cell markers and is a cell-surface glycoprotein involved in cell–cell interactions, cell adhesion, and cell migration. We investigated the role of CD44 expression in both tumor and non-tumor tissues on recurrence of hepatocellular carcinoma (HCC).

Methods

Forty-eight patients with HCC who underwent hepatic resection at our institution were enrolled in this study. CD44 expressions in both tumor and non-tumor tissues were examined using real time reverse transcription-polymerase chain reaction. The patients were divided into two groups: high and low gene-expression group, based on the CD44 expression level. We compared the clinicopathological factors between the high expression and low expression groups in both tumor and non-tumor tissues.

Results

In the tumor tissues, the gene-expression levels of CD44 did not correlate with any clinicopathological parameters. The disease-free survival rate showed no significant difference between the two groups. In non-tumor tissues, although there was no significant relationship between the CD44 expression levels and clinicopathological factors, disease-free survival rate in the CD44 low expression group was significantly better than that in the CD44 high expression group (P < 0.05). In multivariate analysis, the risk factors in tumor recurrence were presence of microscopic portal invasion and high expression level of CD44.

Conclusion

The CD44 expressions in the non-tumor tissues may predict HCC recurrence.

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Abbreviations

HCC:

Hepatocellular carcinoma

RT-PCR:

Reverse transcription-polymerase chain reaction

GAPDH:

Glyceraldehyde-3-phosphate dehydrogenase

AST:

Aspartate aminotransferase

ALT:

Alanine transaminase

WBC:

White blood cell

PT:

Prothrombin time

ICGR15 :

Indocyanine green retention rate at 15 min

AFP:

Alpha-fetoprotein

DCP:

Des-gamma-carboxy prothrombin

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Acknowledgments

This work was supported in part by Grants-in-Aid for Scientific Research (B) (20390359) and for Scientific Research (C) (22591506), and Grant-in-Aid for Challenging Exploratory Research (22659233 and 22659243), Japan Society for the Promotion of Science. This work was also supported in part by a grant from the Cancer Research Project Cooperated by TAIHO Pharmaceutical Co., LTD., and the University of Tokushima.

Conflict of interest

All authors declare that they have no conflict of interest.

Author information

Authors and Affiliations

  1. Department of Surgery, Institute of Health Biosciences, The University of Tokushima, 3-18-15 Kuramoto-cho, Tokushima, 770-8503, Japan

    Lkhagva-Ochir Tovuu, Satoru Imura, Tohru Utsunomiya, Yuji Morine, Tetsuya Ikemoto, Yusuke Arakawa, Hiroki Mori, Jun Hanaoka, Mami Kanamoto, Koji Sugimoto, Shuichi Iwahashi, Yu Saito, Shinichiro Yamada, Michihito Asanoma, Hidenori Miyake & Mitsuo Shimada

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  1. Lkhagva-Ochir Tovuu
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  2. Satoru Imura
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  3. Tohru Utsunomiya
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  4. Yuji Morine
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  5. Tetsuya Ikemoto
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  6. Yusuke Arakawa
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  7. Hiroki Mori
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  8. Jun Hanaoka
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  9. Mami Kanamoto
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  10. Koji Sugimoto
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  11. Shuichi Iwahashi
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  12. Yu Saito
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  13. Shinichiro Yamada
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  14. Michihito Asanoma
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  15. Hidenori Miyake
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  16. Mitsuo Shimada
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Corresponding author

Correspondence to Satoru Imura.

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Cite this article

Tovuu, LO., Imura, S., Utsunomiya, T. et al. Role of CD44 expression in non-tumor tissue on intrahepatic recurrence of hepatocellular carcinoma. Int J Clin Oncol 18, 651–656 (2013). https://doi.org/10.1007/s10147-012-0432-6

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  • DOI: https://doi.org/10.1007/s10147-012-0432-6

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