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An overview of genome engineering in plants, including its scope, technologies, progress and grand challenges

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Abstract

Genome editing is a useful, adaptable, and favored technique for both functional genomics and crop enhancement. Over the years, rapidly evolving genome editing technologies, including clustered regularly interspaced short palindromic repeats/CRISPR-associated protein (CRISPR/Cas), transcription activator-like effector nucleases (TALENs), and zinc finger nucleases (ZFNs), have shown broad application prospects in gene function research and improvement of critical agronomic traits in many crops. These technologies have also opened up opportunities for plant breeding. These techniques provide excellent chances for the quick modification of crops and the advancement of plant science in the future. The current review describes various genome editing techniques and how they function, particularly CRISPR/Cas9 systems, which can contribute significantly to the most accurate characterization of genomic rearrangement and plant gene functions as well as the enhancement of critical traits in field crops. To accelerate the use of gene-editing technologies for crop enhancement, the speed editing strategy of gene-family members was designed. As it permits genome editing in numerous biological systems, the CRISPR technology provides a valuable edge in this regard that particularly captures the attention of scientists.

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Source: Adapted from Zaidi et al. (2017) © 2017. Reproduced with the permission of Elsevier

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Acknowledgements

The authors are thankful to HEC Islamabad for supporting this study.

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Authors and Affiliations

  1. College of Biological Sciences, China Agricultural University, Beijing, 100083, China

    Muhammad Sufyan & Naveed Mahmood

  2. State Key Laboratory of Grassland Agro-Ecosystems, Center for Grassland Microbiome, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou, 730000, China

    Umar Daraz

  3. Department of Botant, Government College Women University, Sialkot, Pakistan

    Sajjad Hyder

  4. Department of Agronomy, Faculty of Agriculture and Environment, The Islamia University of Bahawalpur, Bahawalpur, 63100, Pakistan

    Usman Zulfiqar & Rashid Iqbal

  5. Center of Research, Faculty of Engineering, Future University in Egypt, New Cairo, 11835, Egypt

    Sayed M. Eldin

  6. School of Environmental Sciences and Engineering, NCEPU, Beijing, China

    Farzana Rafiq

  7. Institute of Geographic Sciences and Natural Resources Research, CAS, Beijing, China

    Khurram Shahzad

  8. National Institute for Genomics and Advanced Biotechnology, Park Road, Islamabad, Pakistan

    Muhammad Uzair

  9. Department of Plant Breeding and Genetics, The University of Haripur, Haripur, 22620, Pakistan

    Sajid Fiaz

  10. Center for Plant Sciences and Biodiversity, University of Swat, Charbagh, 19120, Pakistan

    Iftikhar Ali

  11. Department of Genetics and Development, Columbia University Irving Medical Center, New York, NY, 10032, USA

    Iftikhar Ali

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  1. Muhammad Sufyan
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Contributions

MS, UD, IA and RI designed the study, IA, FR, NM, KS, MU, SH, UZ, SME, and SF analyzed the data and edited the manuscript. All authors approved the final version.

Corresponding authors

Correspondence to Rashid Iqbal or Iftikhar Ali.

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Sufyan, M., Daraz, U., Hyder, S. et al. An overview of genome engineering in plants, including its scope, technologies, progress and grand challenges. Funct Integr Genomics 23, 119 (2023). https://doi.org/10.1007/s10142-023-01036-w

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