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Reconstitution of Sperm Nuclei of Zebrafish (Danio rerio) in Xenopus Egg Extracts

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Abstract:

Cell-free extracts of Xenopus eggs cause cyclic change in permeabilized sperm nucleus, nuclear envelope breakdown, chromosome condensation, and reformation of nuclei. In this study, the ability of cell-free extracts to cause similar changes in zebrafish sperm was examined. When lysolecithin-treated sperm from zebrafish were incubated in Xenopus egg extracts, a series of changes in sperm nuclear morphology were observed periodically. These changes correlated with maturation-promoting factor (MPF) activity. Furthermore, sperm nuclei of zebrafish replicated DNA during reconstitution in Xenopus egg extracts. These results showed that cell-free extracts of Xenopus egg possess the ability to cause cell-cycle-dependent changes in zebrafish sperm, implying the possibility of generating transgenic zebrafish in a similar way to transgenic Xenopus.

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Authors and Affiliations

  1. Department of Biology and Geosciences, Faculty of Science, Shizuoka University, Shizuoka 422-8529, Japan, , , , , , JP

    Jungo Imahara, Mika Tokumoto, Katsutoshi Ishikawa & Toshinobu Tokumoto

  2. Laboratory of Reproductive Biology, National Institute for Basic Biology, Okazaki 444-8585, Japan, , , , , , JP

    Yoshitaka Nagahama

Authors
  1. Jungo Imahara
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  2. Mika Tokumoto
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  3. Yoshitaka Nagahama
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  4. Katsutoshi Ishikawa
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  5. Toshinobu Tokumoto
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Received October 21, 1999; accepted July 18, 2000.

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Imahara, J., Tokumoto, M., Nagahama, Y. et al. Reconstitution of Sperm Nuclei of Zebrafish (Danio rerio) in Xenopus Egg Extracts. Mar. Biotechnol. 3, 53–57 (2001). https://doi.org/10.1007/s101260000047

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  • DOI: https://doi.org/10.1007/s101260000047

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