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Influences of Culture Temperature on the Growth, Lipid Content and Fatty Acid Composition of Aurantiochytrium sp. Strain mh0186

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Abstract

The growth, lipid content, and fatty acid composition of Aurantiochytrium sp. strain mh0186 at different temperatures were investigated. Strain mh0186 grew well at 15–30°C, but weakly at 10°C. The biomass at 15–30°C was significantly higher than at 10 and 35°C, and the total lipid at 15–35°C was significantly higher than that at 10°C. The amount of DHA in the total fatty acid was highest at 10°C and decreased in response to temperature increase. The content of DHA (mg/g-dry cell weight) at 15–30°C were significantly higher than those at 35°C and those at 15–25°C were significantly higher than those at 10 and 35°C. The DHA yield at 15–35°C was significantly higher than those at 10 and 35°C. Unsaturation of fatty acid was regulated by temperature and was enhanced in response to temperature decrease. The ratio of DHA to DPA varied at different temperatures.

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Authors and Affiliations

  1. Laboratory of Marine Bioscience, Faculty of Agriculture, Miyazaki University, 1-1 Gakuenkibanadainishi, Miyazaki, 889-2192, Japan

    Yousuke Taoka, Naoki Nagano & Masahiro Hayashi

  2. Nippon Suisan Kaisha Ltd., 2-6-2 Otemachi, Chiyoda-ku, Tokyo, 100-8686, Japan

    Yuji Okita, Hitoshi Izumida & Shinichi Sugimoto

Authors
  1. Yousuke Taoka
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  2. Naoki Nagano
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  3. Yuji Okita
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  4. Hitoshi Izumida
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  5. Shinichi Sugimoto
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  6. Masahiro Hayashi
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Correspondence to Masahiro Hayashi.

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Taoka, Y., Nagano, N., Okita, Y. et al. Influences of Culture Temperature on the Growth, Lipid Content and Fatty Acid Composition of Aurantiochytrium sp. Strain mh0186. Mar Biotechnol 11, 368–374 (2009). https://doi.org/10.1007/s10126-008-9151-4

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  • DOI: https://doi.org/10.1007/s10126-008-9151-4

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