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Bioaccumulation of lead, chromium, and nickel by bacteria from three different genera isolated from industrial effluent

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Abstract

The potential of indigenous bacterial strains to accumulate three metals (Cr, Ni, Pb) was exploited here to remediate the polluted environment. In the present study, metal resistance profiles identified three most potential isolates which could tolerate 700–1000 μg/ml of Ni, 500–1000 μg/ml of Cr, and 1000–1600 μg/ml of Pb. These three bacterial strains were identified as Stenotrophomonas sp. MB339, Klebsiella pneumoniae MB361, and Staphylococcus sp. MB371. UV-Visible and atomic absorption spectrophotometric (AAS) analysis revealed gradual increase in percentage accumulation with increase in time due to increased biomass. Quantitative assessments exhibited maximum removal of Cr (83.51%) by Klebsiella pneumoniae MB361, Pb (85.30%), and Ni (48.78%) by Stenotrophomonas MB339, at neutral pH and 37 °C, whereas Staphylococcus sp. MB371 sorbed 88.33% of Pb at slightly acidic pH. The present study therefore supports the effective utilization of indigenous bacteria for comprehensive treatment of metal-rich industrial effluents.

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References

  • Abd-Alla MH, Morsy FM, El-Enany AWE, Ohyama T (2012) Isolation and characterization of a heavy-metal-resistant isolate of Rhizobium leguminosarum bv. viciae potentially applicable for biosorption of Cd2+ and Co2+. Int Biodeterior Biodegrad 67:48–55

    CAS  Google Scholar 

  • Abourached C, Catal T, Liu H (2014) Efficacy of single-chamber microbial fuel cells for removal of cadmium and zinc with simultaneous electricity production. Water Res 51:228–233

    CAS  PubMed  Google Scholar 

  • Algarra M, Vázquez MI, Alonso B, Casado CM, Casado J, Benavente J (2014) Characterization of an engineered cellulose based membrane by thiol dendrimer for heavy metals removal. Chem Eng J 253:472–477

    CAS  Google Scholar 

  • Arivalagan P, Singaraj D, Haridass V, Kaliannan T (2014) Removal of cadmium from aqueous solution by batch studies using Bacillus cereus. Ecol Eng 71:728–735

    Google Scholar 

  • Aslam F, Yasmin A, Thomas T (2018) Essential gene clusters identified in Stenotrophomonas MB339 for multiple metal/antibiotic resistance and xenobiotic degradation. Curr Microbiol 75(11):1484–1492

  • Banerjee G, Pandey S, Ray AK, Kumar R (2015) Bioremediation of heavy metals by a novel bacterial strain Enterobacter cloacae and its antioxidant enzyme activity, flocculants production, and protein expression in presence of lead, cadmium, and nickel. Water Air Soil Pollut 226:91

    Google Scholar 

  • Barka N, Abdennouri M, El Makhfouk M, Qourzal S (2013) Biosorption characteristics of cadmium and lead onto eco-friendly dried cactus (Opuntia ficus indica). J Environ Chem Eng 1:144–149

    CAS  Google Scholar 

  • Bayramoglu G, Arıca MY (2008) Removal of heavy mercury (II), cadmium (II) and zinc (II) metal ions by live and heat inactivated Lentinus edodes pellets. Chem Eng J 143:133–140

    CAS  Google Scholar 

  • Beveridge T, Hughes M, Lee H (1997) Metal-microbe interactions: contemporary approaches. Adv Microb Physiol 38:177–243

    CAS  PubMed  Google Scholar 

  • Bibi A, Farooq U, Mirza N, Khan A, Sarwar R, Alam A, Mahmood Q (2014) Excessive chromium may cause dietary toxicity in parsley (Petroselinum crispum). Toxicol Environ Chem 96(2):287–295

    CAS  Google Scholar 

  • Churchill S, Walters J, Churchill P (1995) Sorption of heavy metals by prepared bacterial cell surfaces. J Environ Eng 121:706–711

    CAS  Google Scholar 

  • Colak F, Atar N, Olgun A (2011) Biosorption of lead from aqueous solutions by Bacillus strains possessing heavy-metal resistance. Chem Eng J 173:422–428

    CAS  Google Scholar 

  • Deeb BE, Altalhi AD (2009) Degradative plasmid and heavy metal resistance plasmid naturally coexist in phenol and cyanide assimilating bacteria. Am J Biochem Biotechnol 5(2):84–93

    Google Scholar 

  • Devika MV, Thatheyus AJ, Ramya D (2014) Bioremoval of nickel using Pseudomonas aeruginosa. Ann Res Rev Biol 4(3):538–546

    Google Scholar 

  • Dexilin M, Congeevaram S, Dhanarani S (2007) Biosorption of chromium and nickel by heavy metal resistant fungal and bacterial isolates. J Hazard Mater 146:270–277

    PubMed  Google Scholar 

  • Eiband MM, Kamélia CDA, Gama K, de Melo JV, Martínez-Huitle CA, Ferro S (2014) Elimination of Pb2+ through electrocoagulation: applicability of adsorptive stripping voltammetry for monitoring the lead concentration during its elimination. J Electroanal Chem 717:213–218

    Google Scholar 

  • El-Gendy MM, El-Bondkly AM (2016) Evaluation and enhancement of heavy metals bioremediation in aqueous solutions by Nocardiopsis sp. MORSY1948, and Nocardia sp. MORSY2014. Braz J Microbiol 47:571–586

    CAS  PubMed  PubMed Central  Google Scholar 

  • Ergul-Ulger Z, Ozkan AD, Tunca E, Atasagun S, Tekinay T (2014) Chromium (VI) biosorption and bioaccumulation by live and acid-modified biomass of a novel Morganella morganii isolate. Sep Sci Technol 49(6):907–914

    CAS  Google Scholar 

  • Gabr R, Hassan S, Shoreit A (2008) Biosorption of lead and nickel by living and non-living cells of Pseudomonas aeruginosa ASU 6a. Int Biodeterior Biodegrad 62:195–203

    CAS  Google Scholar 

  • Ganje TJ, Page AL (1974) Rapid acid dissolution of plant tissue for cadmium determination by atomic absorption spectrophotometry. Atom Absorpt Newslett 13(6):131–134

    CAS  Google Scholar 

  • Goyal N, Jain SC, Banerjee UC (2003) Comparative studies on the microbial adsorption of heavy metals. Adv Environ Res 7(2):311–319

    CAS  Google Scholar 

  • Gupta VK, Nayak A, Agarwal S (2015) Bioadsorbents for remediation of heavy metals: current status and their future prospects. Environ Eng Res 20(1):1–18

    Google Scholar 

  • Holt JG, Krieg NR, Sneath PH, Staley JT, Williams ST (1994) Group 19. Regular, nonsporing Gram-positive rods. Bergey’s manual of determinative bacteriology, 9th edn. Williams & Wilkins Co., Baltimore, p 566

    Google Scholar 

  • Hossain A, Aditya G (2013) Cadmium biosorption potential of shell dust of the fresh water invasive snail Physa acuta. J Environ Chem Eng 1:574–580

    CAS  Google Scholar 

  • Huang F, Dang Z, Guo CL, Lu GN, Gu RR, Liu HJ, Zhang H (2013) Biosorption of Cd (II) by live and dead cells of Bacillus cereus RC-1 isolated from cadmium contaminated soil. Colloids Surf B 107:11–18

    CAS  Google Scholar 

  • Huang F, Guo CL, Lu GN, Yi XY, Zhu LD, Dang Z (2014) Bioaccumulation characterization of cadmium by growing Bacillus cereus RC-1 and its mechanism. Chemosphere 109:134–142

    CAS  PubMed  Google Scholar 

  • Jiménez-Ortega V, Barquilla PC, Fernández-Mateos P, Cardinali DP, Esquifino AI (2012) Cadmium as an endocrine disruptor: correlation with anterior pituitary redox and circadian clock mechanisms and prevention by melatonin. Free Radic Biol Med 53(12):2287–2297

    PubMed  Google Scholar 

  • Kaewehai S, Prasertson P (2002) Biosorption of heavy metals. J Sci Technol 24(3):422–430

    Google Scholar 

  • Marc M, Burton JP, Reid G (2012) Bioremediation and tolerance of humans to heavy metals through microbial processes. Appl Environ Microbiol 78(18):6397–6404

    Google Scholar 

  • Masood F, Malik A (2011) Biosorption of metal ions from aqueous solution and tannery effluent by Bacillus sp. FM1. J Environ Sci Health A 46:1667–1674

    CAS  Google Scholar 

  • McFarlane JS, Davis CL, Lamb AL (2018) Staphylopine, pseudopaline and yersinopine dehydrogenases: a structural and kinetic analysis of a new functional class of opine dehydrogenase. J Biol Chem 293:8009–8019

    CAS  PubMed  PubMed Central  Google Scholar 

  • Odokuma LO and Series IL (2012) The genius in microbes: an indispensible for the management of xenobiotic mediated environmental flux. University of Port Harcourt Inaugral Lecture, pp 87

  • Ojuederie O, Babalola O (2017) Microbial and plant-assisted bioremediation of heavy metal polluted environments. A review. Int J Environ Res Public Health 14(12):1504

    PubMed Central  Google Scholar 

  • Ok Y, Yang J, Zhang Y, Kim S, Chung D (2007) Heavy metal adsorption by a formulated zeolite-Portland cement mixture. J Hazard Mater 147:91–96

    CAS  PubMed  Google Scholar 

  • Olaniran AO, Balgobind A, Pillay B (2013) Bioavailability of heavy metals in soil: impact on microbial biodegradation of organic compounds and possible improvement strategies. Int J Mol Sci 14:10197–10228

    PubMed  PubMed Central  Google Scholar 

  • Olmezoglu E, Herand B, Oncel M, Tunc K, Ozkan M (2012) Copper bioremoval by novel bacterial isolates and their identification by 16S rRNA gene sequence analysis. Turk J Biol 36:469–476

    CAS  Google Scholar 

  • Oves M, Khan M, Zaidi A (2013) Biosorption of heavy metals by Bacillus thuringiensis strain OSM29 originating from industrial effluent contaminated north Indian soil. Saudi J Biol Sci 20:121–129

    CAS  PubMed  Google Scholar 

  • Oyewo OA, Agboola O, Onyango MS, Popoola P and Bobape MF (2018) Current methods for the remediation of acid mine drainage including continuous removal of metal from wastewater and mine dump. In Bio-Geotechnologies for mine site rehabilitation. Elsevier, pp 103–114

  • Pandiyan S, Mahendradas D (2011) Application of bacteria to remove Ni (II) ions from aqueous solution. Eur J Sci Res 52:345–358

    Google Scholar 

  • Poornima M, Kumar RS, Thomas PD (2014) Isolation and molecular characterization of bacterial strains from tannery effluent and reduction of chromium. Int J Curr Microbiol App Sci 3(4):530–538

    Google Scholar 

  • Reis KC, Silva CF, Duarte WF, Schwan RF (2014) Bioaccumulation of Fe3+ by bacteria isolated from soil and fermented foods for use in bioremediation processes. Afr J Microbiol Res 8(26):2513–2521

    Google Scholar 

  • Ren WX, Li PJ, Geng Y, Li XJ (2009) Biological leaching of heavy metals from a contaminated soil by Aspergillus niger. J Hazard Mater 167:164–169

    CAS  PubMed  Google Scholar 

  • Sahu S, Roy B, Varghese TA (2015) Bioremediation of lead using indigenous bacterial consortium isolated from tannery effluent-a bioreactor based approach. Int J Curr Microbiol App Sci 4(7):919–927

    CAS  Google Scholar 

  • Selvi AT, Anjugam E, Devi RA, Madhan B, Kannappan S, Chandrasekaran B (2012) Isolation and characterization of bacteria from tannery effluent treatment plant and their tolerance to heavy metals and antibiotics. Asian J Exp Biol Sci 3(1):34–41

    CAS  Google Scholar 

  • Shim HY, Lee KS, Lee DS, Jeon DS, Park MS, Shin JS, Chung DY (2014) Application of electrocoagulation and electrolysis on the precipitation of heavy metals and particulate solids in washwater from the soil washing. J Agri Chem Environ 3(4):130

    Google Scholar 

  • Sibi G (2016) Biosorption of chromium from electroplating and galvanizing industrial effluents under extreme conditions using Chlorella vulgaris. Green Energy Environ 1(2):172–177

    Google Scholar 

  • Takahashi C, Turner A, Millward G, Glegg G (2012) Persistence and metallic composition of paint particles in sediments from a tidal inlet. Marine Poll Bull 64:133–137

    CAS  Google Scholar 

  • Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30(12):2725–2729

    CAS  PubMed  PubMed Central  Google Scholar 

  • Thomas KJ III, Rice CV (2014) Revised model of calcium and magnesium binding to the bacterial cell wall. Biometals 27(6):1361–1370

    CAS  PubMed  PubMed Central  Google Scholar 

  • Thyssen JP, Gawkrodger DJ, White IR et al (2013) Coin exposure may cause allergic nickel dermatitis: a review. Contact Dermatitis 68:148–157

    Google Scholar 

  • Tian H, Lu L, Cheng K, Hao J, Zhao D, Wang Y, Jia W, Qiu P (2012) Anthropogenic atmospheric nickel emissions and its distribution characteristics in China. Sci Total Environ 417–418:148–157

    PubMed  Google Scholar 

  • Uslu G, Tanyol M (2006) Equilibrium and thermodynamic parameters of single and binary mixture biosorption of lead (II) and copper (II) ions onto Pseudomonas putida: effect of temperature. J Hazard Mater B 135:87–93

    CAS  Google Scholar 

  • Wang J, Chen C (2009) Biosorbents for heavy metals removal and their future. Biotechnol Adv 27:195–226

    PubMed  Google Scholar 

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Authors and Affiliations

  1. Microbiology & Biotechnology Research Lab, Department of Environmental Sciences, Fatima Jinnah Women University, Rawalpindi, Pakistan

    Fozia Aslam, Azra Yasmin & Sana Sohail

  2. Department of Biotechnology, Fatima Jinnah Women University, Rawalpindi, Pakistan

    Azra Yasmin

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  1. Fozia Aslam
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Correspondence to Fozia Aslam.

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Aslam, F., Yasmin, A. & Sohail, S. Bioaccumulation of lead, chromium, and nickel by bacteria from three different genera isolated from industrial effluent. Int Microbiol 23, 253–261 (2020). https://doi.org/10.1007/s10123-019-00098-w

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