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Micro-CT analysis and leakage of bioceramic retrofillings after ultrasonic and Er:YAG laser cavity preparations: an in vitro study

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Abstract

The purpose of the study was to determine the influence of preparation techniques on marginal adaptation and sealing of Biodentine™ and TotalFill® RRM bioceramic retrograde fillings. Fifty-two single-root teeth extracted for periodontal reasons were used. Root canals were instrumented using Reciproc Blue #25 and obturated using a single cone technique with an AH Plus® root canal sealer. Retrograde cavities were prepared with Piezomed device (Piezo), Er:YAG laser in short-pulse(SP) and quantum square pulse(QSP) modes and filled with Biodentine™ (BD) or TotalFill® RRM (TF). There were 6 groups (n=8): (1) Piezo BD, (2) Piezo TF, (3) SP BD, (4) SP TF, (5) QSP BD, and (6) QSP TF, and positive and negative controls (n=2). Micro-CT analysis was performed on two samples from each group. Percentage volumes of internal and external voids in apical 1.5 mm were determined. Rhodamine B dye leakage was done on six samples. The samples were cut longitudinally and examined under a stereomicroscope. Digital recordings were analyzed in ImageJ software. The deepest penetration of color in mm was recorded. The data were statistically analyzed using ANOVA and Duncan’s test at the level of significance α=0.05. TotalFill® RRM performed significantly better than Biodentine™ in terms of sealing (p<0.05) and marginal adaptation, as evaluated by micro-CT. Sealing was significantly better in SP compared to QSP mode preparations (p<0.05). Differences between Piezomed and laser modes were not significantly different (p>0.05). Sealing was statistically significantly better with TotalFill® RRM compared to Biodentine™ and in Er:YAG SP preparations compared to Er:YAG QSP.

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Authors and Affiliations

  1. Private Dental Office, Pula, Croatia

    Luka Marković

  2. Department of Endodontics and Restorative Dentistry, School of Dental Medicine, University of Zagreb, Zagreb, Croatia

    Ana Ivanišević & Jurica Matijević

  3. Applied Oral Sciences and Community Dental Care, Faculty of Dentistry, The University of Hong Kong, SAR, Hong Kong, People’s Republic of China

    Rosalind Sin Man Chan & James Kit Hon Tsoi

  4. Department for Endodontics and Restorative Dentistry, School of Dental Medicine, University of Rijeka, Rijeka, Croatia

    Damir Šnjarić

  5. Department of Paediatric and Preventive Dentistry, Faculty of Dental Medicine, University “Ss Cyril and Methodius” Skopje, Skopje, Republic of Macedonia

    Elizabeta Gjorgievska

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  1. Luka Marković
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  2. Ana Ivanišević
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  4. Rosalind Sin Man Chan
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Contributions

Conceptualization: Ana Ivanišević and Elizabeta Gjorgievska; sample preparation: Luka Marković and Damir Šnjarić; methodology: Rosalind Sin Man Chan, Jurica Matijević, and Ana Ivanišević; formal analysis and investigation: Rosalind Sin Man Chan, Ana Ivanišević, and Luka Marković; writing—original draft preparation: Ana Ivanišević; writing—review and editing: Jurica Matijević and Rosalind Sin Man Chan; resources: James Kit Hon Tsoi, Luka Marković; supervision: Ana Ivanišević.

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Correspondence to Ana Ivanišević.

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The research was approved by the Ethical Committee of the School of Dental Medicine, University of Zagreb (number 05-PA-30-IX-6/2022).

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Marković, L., Ivanišević, A., Matijević, J. et al. Micro-CT analysis and leakage of bioceramic retrofillings after ultrasonic and Er:YAG laser cavity preparations: an in vitro study. Lasers Med Sci 38, 145 (2023). https://doi.org/10.1007/s10103-023-03809-y

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