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The effect of Er,Cr:YSGG laser on periodontopathic bacteria elimination: an in vitro study

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Abstract

In vitro bacterial elimination using the erbium, chromium: yttrium–scandium–gallium–garnet (Er,Cr:YSGG) laser against periodontopathic bacteria was investigated. Bacterial suspensions of Aggregatibacter actinomycetemcomitans and Porphyromonas gingivalis were spread on agar plates and the Er,Cr:YSGG laser was applied at 40 mJ pulse energy for durations of 30 s, 60 s, and 90 s. The agar plates were incubated, and growth inhibition zones were assessed. Optimal laser irradiation durations to achieve maximal bacterial elimination were evaluated using laser ablation on the bacterial colonies. The remaining viable bacteria were determined by the colony-forming unit (CFU) counting method. Growth inhibition zones were observed at all irradiation durations for both A. actinomycetemcomitans and P. gingivalis. Mean logarithmic values of CFU/ml after bacterial colony irradiation for 0 s (control), 12 s × 1 lap, 24 s × 1 lap, 48 s × 1 lap, and 24 s × 2 laps were 8.82 ± 0.35, 7.31 ± 0.94, 6.32 ± 0.61, 3.17 ± 2.90, and 0.00, respectively, for A. actinomycetemcomitans and 9.83 ± 0.50, 9.42 ± 0.11, 6.90 ± 1.60, 2.33 ± 3.19, and 0.00 for P. gingivalis. Significant differences were found between the control group and the two irradiated groups 48 s × 1 lap and 24 s × 2 laps (p < 0.05), and also between irradiated groups 12 s × 1 lap and 24 s × 2 laps (p < 0.05). An Er,Cr:YSGG laser with power setting 1.5 W and 30 Hz frequency showed potential for bacterial elimination against A. actinomycetemcomitans and P. gingivalis in vitro. Significant bacterial elimination (> 99.99%) was observed after 48 s of irradiation.

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Funding

This study was supported by the Faculty of Dentistry, Mahidol University Research Fund (DTRS-GG-2021–02).

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Authors and Affiliations

  1. Dental Department, Selaphum Hospital, Roi Et, 45120, Thailand

    Pornnutchar Sethasathien

  2. Department of Oral Microbiology, Faculty of Dentistry, Mahidol University, Yothi, Bangkok, 10400, Thailand

    Pornpen Tantivitayakul

  3. Department of Oral Medicine and Periodontology, Faculty of Dentistry, Mahidol University, Yothi, Bangkok, 10400, Thailand

    Thitiwan Teparat-Burana

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  1. Pornnutchar Sethasathien
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Contributions

Pornnutchar Sethasathien conducted the experiments, collected data, performed the statistical analysis, and wrote the first draft of the manuscript. Pornpen Tantivitayakul conceptualized, designed, and supervised the study. Thitiwan Teparat-Burana was also involved in study conceptualization, design and supervision, and authorized submission for publication. All authors reviewed and approved the final version of the manuscript.

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Correspondence to Thitiwan Teparat-Burana.

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Sethasathien, P., Tantivitayakul, P. & Teparat-Burana, T. The effect of Er,Cr:YSGG laser on periodontopathic bacteria elimination: an in vitro study. Lasers Med Sci 37, 2763–2771 (2022). https://doi.org/10.1007/s10103-022-03573-5

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